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Offlinecumdumpster
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Registered: 10/03/20
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First time AGAR grow questions!
    #27155438 - 01/18/21 03:09 PM (1 month, 7 days ago)

Just getting started on my very first attempt at growing. I've done a fair amount of research on here and have some basic questions which I couldn't find super clear answers to yet and would appreciate a nudge in the right direction.

The steps I'm taking are:

Make agar (now foods agar powder, malt extract, water, two drops green food coloring)
Make SAB
Pour plates in the SAB. I have 20 Petri dishes in sleeves, supposedly sterile.
inoculate plates (with spores from syringe and disposable sterile inoculation loops)
Then transfer them to grain spawn then bulk substrate.

My first questions are:
1) I'm planning to pour all the plates. Should I only inoculate a couple of plates first (~5 plates)? And then see what happens? Then transfer healthy sections to my remaining plates?
2) How long does it take for plates to usually be ready to send to grain spawn?
3) How should the plates be stored after initial inoculation from a syringe? Is room temperature ok? Any requirements for light at this stage?

Thanks in advance! I'd appreciate any links, especially a link to a detailed grow journal


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OfflineHaywire
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27155471 - 01/18/21 03:22 PM (1 month, 7 days ago)

1) it's your life. live like you want to live. if you want to inoculate all, then go ahead. if you want to save some for later, that's fine as well.
2) if you want to use a full plate for inoculation it takes longer than if you just use a small wedge
3) room temp is fine, no light requirements.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: Haywire]
    #27155544 - 01/18/21 03:57 PM (1 month, 7 days ago)

Thanks for the response

I think I'll inoculate maybe 8 first and see what happens with those. I read on here somewhere that it's a good idea to transfer them until it's obvious there is healthy mycelium.

How long of a time period usually passes from inoculation to being ready to drop wedges into grain spawn? Like how long for the mycelium to be ready and what do they look like when they're ready to be transferred?


--------------------
Bob Bop Perano 2024


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OfflineCrackatoaS
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27155606 - 01/18/21 04:27 PM (1 month, 7 days ago)

I would do as you said, knock up a few and keep the rest for transfers. You're gonna have to do  some transfers, you hardly ever get that lucky but it happens.
Inoculating plates from syringe can take a few. Then transfer a couple times, it's hard to really say there's several factors you can't really put a time line on.
After inoculating just wrap and sit it somewhere. Room temp and normal light is fine.
I can't think of a specific grow, you got LAGM 2021, you'll see alot there. But here's a link to cleaning and isolating on agar. It's a must read. https://www.shroomery.org/forums/showflat.php/Number/18430998


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: Crackatoa]
    #27155618 - 01/18/21 04:32 PM (1 month, 7 days ago)

Crackatoa thanks a lot for the response and the link. I'd just like to clarify, by wrap, you mean wrap the petris with something like saran wrap?


--------------------
Bob Bop Perano 2024


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OfflineCrackatoaS
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Re: First time AGAR grow questions! [Re: cumdumpster] * 1
    #27155645 - 01/18/21 04:45 PM (1 month, 7 days ago)

Yes, I cut my saran wrap in 1 inch tape roll's and wrap around a couple times. You're welcome, always here to help.


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OfflineCocaineBuffet
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Re: First time AGAR grow questions! [Re: Crackatoa]
    #27156141 - 01/18/21 09:03 PM (1 month, 7 days ago)

When pouring plates I like stack them as high as I can if I am in a SAB and let it sit overnight. That way overnight the condensation rises to the top so the plates in the middle should be more clear.

Additionally agar is poured at a high temp and the high temp could be too hot to let the spores thrive so that is another reason I wait until they are cool.


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OfflineHaywire
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27156657 - 01/19/21 04:55 AM (1 month, 6 days ago)

Quote:

cumdumpster said:
Thanks for the response

I think I'll inoculate maybe 8 first and see what happens with those. I read on here somewhere that it's a good idea to transfer them until it's obvious there is healthy mycelium.

How long of a time period usually passes from inoculation to being ready to drop wedges into grain spawn? Like how long for the mycelium to be ready and what do they look like when they're ready to be transferred?




don't focus too much on 'how long in general does it take'.
Learn to identify a good plate, sector, ready spawn by sight not by time.
good things take time, unless they stall...


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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Invisiblebigfootscreepyuncl
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Re: First time AGAR grow questions! [Re: Haywire]
    #27156701 - 01/19/21 05:43 AM (1 month, 6 days ago)

Everyone before me has given good advice. I'd like to chime in and say that if you're inoculating from a MS syringe I would recommend doing at least 5 plates of each variety you're doing, and to also expect very little return. I do NOT want to sound like a rain cloud or to be discouraging, but I've been working syringes to agar for a couple months now and have a piss poor germination rate.

The ones that have germinated have taken anywhere from 3 days to 16 days.

I like to pour my plates as soon as they hit 120F, stack 'em as high as my SAB will allow and let 'em sit for 24 hours.



Having clean growth is more important than having tomentose or rhizo growth when coming from MS. The reasoning behind this is to keep the gene pool as wide as possible, since you don't know what genetics you're transferring out unless you fruit it. (Meaning, in a perfect world you would have a clean T-1 plate you can put to grain so there is as much genetic variety in there as possible).


Best of luck mate, cheers!


--------------------
Bigfoots LAGM21


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: bigfootscreepyuncl]
    #27178157 - 01/30/21 05:34 PM (26 days, 8 hours ago)

Thanks all for the replies cheers!

I'm 13 days in to three plates, here. What do yall think? i know #3 is shit, not sure what it is on 1 and 2 though







--------------------
Bob Bop Perano 2024


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OfflineCrackatoaS
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27178299 - 01/30/21 07:59 PM (26 days, 5 hours ago)

1 and 2 are satellite contamination from your hand or tool usually.


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: Crackatoa]
    #27178327 - 01/30/21 08:30 PM (26 days, 5 hours ago)

Damn thanks for the heads up... okay, three new plates hopefully in a much more sterile manner, coming up tomorrow. Gonna try no z drag this time, just a drop or two in the center. No harm in adding a few drops right?


--------------------
Bob Bop Perano 2024


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OfflineCocaineBuffet
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27178421 - 01/30/21 09:31 PM (26 days, 4 hours ago)

Your parafilm/plastic wrap seems too not be covering the entire plate. Can you put the wrap on tighter around the circumference of the plate?


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: CocaineBuffet]
    #27181466 - 02/01/21 07:44 PM (24 days, 5 hours ago)

Another pic of #2. Is this hairy stuff mold? I'm afraid it is....



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Bob Bop Perano 2024


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OfflineCrackatoaS
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27181497 - 02/01/21 08:00 PM (24 days, 5 hours ago)

What's it look like without the backlight? Spot on the right is something.


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: Crackatoa]
    #27182831 - 02/02/21 02:35 PM (23 days, 11 hours ago)

Quote:

Crackatoa said:
What's it look like without the backlight? Spot on the right is something.






here's another pic. Maybe this is mycelium? Concerned about all those little black dots though. Do spores grow on agar? First time so i don't have experience identifying everything. Again, the variety is golden teacher.


--------------------
Bob Bop Perano 2024


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Invisiblebigfootscreepyuncl
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27182840 - 02/02/21 02:41 PM (23 days, 11 hours ago)

Quote:

cumdumpster said:
Quote:

Crackatoa said:
What's it look like without the backlight? Spot on the right is something.






here's another pic. Maybe this is mycelium? Concerned about all those little black dots though. Do spores grow on agar? First time so i don't have experience identifying everything. Again, the variety is golden teacher.




That spot on the side is bacteria of some variety, and it does look like the rest is mold. I would toss it and start fresh.

To answer your questions - YES, spores grow on agar. That's like 95% of the reason we use it lol


--------------------
Bigfoots LAGM21


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Offlinecumdumpster
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Re: First time AGAR grow questions! [Re: bigfootscreepyuncl]
    #27182994 - 02/02/21 04:14 PM (23 days, 9 hours ago)

Quote:

Crackatoa said:
What's it look like without the backlight? Spot on the right is something.






here's another pic. Maybe this is mycelium? Concerned about all those little black dots though. Do spores grow on agar? First time so i don't have experience identifying everything. Again, the variety is golden teacher.
Quote:

bigfootscreepyuncl said:
Quote:

cumdumpster said:
Quote:

Crackatoa said:
What's it look like without the backlight? Spot on the right is something.






here's another pic. Maybe this is mycelium? Concerned about all those little black dots though. Do spores grow on agar? First time so i don't have experience identifying everything. Again, the variety is golden teacher.




That spot on the side is bacteria of some variety, and it does look like the rest is mold. I would toss it and start fresh.

To answer your questions - YES, spores grow on agar. That's like 95% of the reason we use it lol






Sheeeeitttt... so the whispy white shit is mold?? :sad:

I wish it wasnt true...


--------------------
Bob Bop Perano 2024


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Invisiblebigfootscreepyuncl
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Re: First time AGAR grow questions! [Re: cumdumpster]
    #27183019 - 02/02/21 04:26 PM (23 days, 9 hours ago)

It might be, but since the (what I suspect is mold) has already started sporulating you're better off not opening it. That's just my $0.02

Wait for someone with more experience to chime in, but I'd scrap it personally.


--------------------
Bigfoots LAGM21


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OfflineCrackatoaS
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Re: First time AGAR grow questions! [Re: bigfootscreepyuncl]
    #27183069 - 02/02/21 04:59 PM (23 days, 8 hours ago)

I would only transfer from that as a last resort and if I did it would be in SAB and not a hood.


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Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Amazon Agar, Malt Extract, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Getting Started

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