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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Crystals
#27150008 - 01/15/21 03:58 PM (3 years, 4 months ago) |
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I'm not sure if this belongs here or in chemistry.
But a while back I had the idea to try to extract crystals from my spent substrate. I soaked an unknown amount of sub in everclear. I let it sit for about a week before straining. I got pretty busy with life and threw this solution in the freezer and forgot about it. I'm now revisiting it months later.
So I took this....
 To cook of the alcohol.
The result looks like this...

So now I'm curious if these are active?
My goal was to minimize waste and just to play around.
Was I successful?
I haven't tried any and I'm not even sure I will, I just like to learn.
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xXxM1NG0xXx
Cultivator


Registered: 07/27/12
Posts: 17
Last seen: 5 months, 26 days
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semi-active possibly, crystals are just mainly salts though.
i.e: i too have made crystals
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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Cool, I'm slightly apprehensive as I'm unsure how to Even dose these, or what I even made for that matter. A friend of mine is teaching me organic chemistry rn so I'm just expiramenting lol it's fun.
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Nichrome
I and I are the roots.



Registered: 12/17/18
Posts: 6,930
Loc: Zone 5
Last seen: 1 hour, 34 minutes
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Salt and sugar.
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“Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh
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Vinci
I'm You



Registered: 08/26/19
Posts: 642
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I did it with a half ounce of dry cubes in everclear, boiled a couple times, soaked for 3 days, shook occasionally, filtered. I didn't evaporate it down, but after an hour in the freezer lot's of crystals precipitated out, floating in the solution. I put a scoop of vitamin c in it to slow oxidization and I just shook it up to distribute crystals then measure out a dose with a syringe, put that bitch in a fruit smoothie. The everclear soothes the nerves for the come up depending on the potency and amount you drink for your dose. That shit was very active for at least a month. I think you likely lose most of the potency when you evap. Edit: This was very helpful to me: http://www.fanaticus.com/mycoalki.htm. Nichrome I like the new profile image
-------------------- Vinci luh you
Edited by Vinci (01/20/21 11:04 AM)
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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Re: Crystals [Re: Vinci]
#27159703 - 01/20/21 02:26 PM (3 years, 3 months ago) |
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Cool, I may try this with the b+ I'm harvesting.
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Cyonic
Stranger



Registered: 12/27/20
Posts: 245
Last seen: 1 year, 3 months
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Re: Crystals [Re: Vinci]
#27179521 - 01/31/21 02:38 PM (3 years, 3 months ago) |
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Quote:
Vinci said: I did it with a half ounce of dry cubes in everclear, boiled a couple times, soaked for 3 days, shook occasionally, filtered. I didn't evaporate it down, but after an hour in the freezer lot's of crystals precipitated out, floating in the solution. I put a scoop of vitamin c in it to slow oxidization and I just shook it up to distribute crystals then measure out a dose with a syringe, put that bitch in a fruit smoothie. The everclear soothes the nerves for the come up depending on the potency and amount you drink for your dose. That shit was very active for at least a month. I think you likely lose most of the potency when you evap. Edit: This was very helpful to me: http://www.fanaticus.com/mycoalki.htm. Nichrome I like the new profile image
Would not be floating. More likey that what you saw was a starch precipitation as opposed to alkaloids. Alkaloids were likely in the alcoholic solution but not falling my out as crystals.
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Cyonic
Stranger



Registered: 12/27/20
Posts: 245
Last seen: 1 year, 3 months
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Quote:
xXxM1NG0xXx said: semi-active possibly, crystals are just mainly salts though.
i.e: i too have made crystals
Quote:
6ixShooter said: I'm not sure if this belongs here or in chemistry.
But a while back I had the idea to try to extract crystals from my spent substrate. I soaked an unknown amount of sub in everclear. I let it sit for about a week before straining. I got pretty busy with life and threw this solution in the freezer and forgot about it. I'm now revisiting it months later.
So I took this....
 To cook of the alcohol.
The result looks like this...

So now I'm curious if these are active?
My goal was to minimize waste and just to play around.
Was I successful?
I haven't tried any and I'm not even sure I will, I just like to learn.
Not likely psilo alkaloids. What type of sub did you extract from?;I will bet that what you have is starch. Not a clear enough pic to tell, but from what you have described, and from my own experience I would think that that is starch.
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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Re: Crystals [Re: Cyonic]
#27179557 - 01/31/21 03:10 PM (3 years, 3 months ago) |
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Brf and I pitched them and chalked it up as a learning experience. I had fun with it.
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Omnipositive
Dr. Stranger


Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
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Psilocybin has to be extracted cold or it with oxidize in the water, then you can vacuum evaporate cold with a vacuum pump. The temperature should be as cold as possible. Ive done it at 10 degrees with no oxidation, at 30 degrees it immediately turns the water blue. This is the cascading blue reaction 💙, if it turns blue thats Psilocybin turning into molecules that resemble Indigo and should be avoided.
For storage, I think freeze drying the crystals with liquid nitrogen would be ideal.
Edited by Omnipositive (01/31/21 06:22 PM)
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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Quote:
Omnipositive said: Psilocybin has to be extracted cold or it with oxidize in the water, then you can vacuum evaporate cold with a vacuum pump. The temperature should be as cold as possible. Ive done it at 10 degrees with no oxidation, at 30 degrees it immediately turns the water blue. This is the cascading blue reaction 💙, if it turns blue thats Psilocybin turning into molecules that resemble Indigo and should be avoided.
For storage, I think freeze drying the crystals with liquid nitrogen would be ideal.
Thanks bro that's pretty sweet. I was un aware of this method. I wanna see that indigo reaction but that would mean I did it wrong. Vacuum filter.. so like a vacuum buchner funnel? I have a few projects going on RN but I may try this method.
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Omnipositive
Dr. Stranger


Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
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I was wrong about the vaccum filtration, kinda.
You will have to freeze dry the final extract.
Has to be frozen to -58 to -112 degrees F. then pull negative pressure. This causes sublimation, turns the frozen h20 directly to water vapor.
Edited by Omnipositive (02/08/21 12:02 AM)
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Omnipositive
Dr. Stranger


Registered: 01/29/21
Posts: 51
Loc: MI
Last seen: 2 years, 2 months
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I pulled vacuum with a 2 stage vacuum pump, I used to use to make wax. The water bubbles and looks like it's boiling but its not.
I'm going to keep my eyes open on the university supply stores for something like this.
https://www.ebay.com/itm/324449589377
-------------------- I don't want to need drugs.
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6ixShooter
Creator of common moulds


Registered: 05/29/20
Posts: 141
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Lmk how it goes. I'm waiting on some fiji to colonize. I'm about to cultivate some enigma, I'm thinking these will be worth trying.
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 9 months
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That is SUGAR!!! Mushrooms are high in sugar, an alcohol extraction will pull the sugar out into the alcohol and once cooled the sugar will precipitate and form crystals, the actives are still in the alcohol. There is a little more to it to get psilocybin or psilocin crystals. It will need to be properly filtered for one to remove the impurities.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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DINKLB3RG
Myco-Breeder


Registered: 06/03/21
Posts: 15
Loc: California
Last seen: 1 year, 10 months
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ethanol extraction. Crystal of the gods. sugar's aren't necessarily bad. just understand how to use them. from what I understand psilocybin and Psilocin aren't super stable when exposed to air. might be better locked up in a trehalose crystal structure like this
Edited by DINKLB3RG (06/03/21 03:18 PM)
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Vinci
I'm You



Registered: 08/26/19
Posts: 642
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Tested?
-------------------- Vinci luh you
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DINKLB3RG
Myco-Breeder


Registered: 06/03/21
Posts: 15
Loc: California
Last seen: 1 year, 10 months
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Re: Crystals [Re: Vinci]
#27334909 - 06/04/21 07:31 AM (2 years, 11 months ago) |
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Oakland is getting a sample for legit analytics. But I can say for certain its active. I can fit about 1/8th equivalent of actives into a size 0 capsule.
Edited by DINKLB3RG (06/04/21 10:21 AM)
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 9 months
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Quote:
DINKLB3RG said: ethanol extraction. Crystal of the gods. sugar's aren't necessarily bad. just understand how to use them. from what I understand psilocybin and Psilocin aren't super stable when exposed to air. might be better locked up in a trehalose crystal structure like this

It's a complex process and can't be done with ethanol alone, you have to be able to strip the halogen from it in order to get crystals. Here's an excerpt from Albert Hoffman on the subject.
1. A PROCESS FOR OBTAINING THE PSYCHOTROPICALLY ACITVE COMPOUNDS PSILOCYBING AND PSILOCIN, WHICH COMPRISES EXTRACTING THE ACTIVE PRINCIPLES FROM FUNGAL MATERIAL OF ONE OF THE SPECIES PSILOCYBE MEXICANA HEIM, STROPHARIA CUBENSIS EARLE, PSILOCYBE SEMPERVIVA HEIM AND CAILLEUX, PSILOCYBE CAERULESCENS MURRILL VAR. MAZATECORUM HEIM, PSILOCYBE ZAPOTECORUM HEIM, PSILOCYBE AZTECORUM HEIM AND PSILOCYBE CAERULESCENS MURRIL VAR NIGRIPES HEIM, BY MEANS OF THE EXTRACTANT ACTION ON SAID MATERIAL OF A WATERMISCIBLE ORGANIC SOLVENT FOR SUCH ACTIVE PRINCIPLES SAID SOLVENT BEING SELECTED FROM THEGROUP CONSISTING OF WATER, LOWER ALIPHATIC ALCOHOL, AND A MIXTURE OF WATER AND LOWER ALIPHATIC ALCOHOL, SEVERALLY ISOLATING THE ACTIVE PRINCIPLES FROM THE RESULTING EXTRACT, AND TREATING THE SO-OBTAINED MATERIAL WITH A SILVER IONS-YIELDING HALOGEN-ACCEPTOR AND THUS FREEING THE MATERIAL OF HALOGEN, WHEREBY THE COMPOUNDS PSILOCYBIN AND PSILOCIN AND OBTAINED
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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DINKLB3RG
Myco-Breeder



Registered: 06/03/21
Posts: 15
Loc: California
Last seen: 1 year, 10 months
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Re: Crystals [Re: Doc9151]
#27338647 - 06/07/21 09:19 AM (2 years, 11 months ago) |
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Solvent was the same as described. (wet ethanol)
The ISOLATE won't crystallize if you don't manipulate it. That's correct.
this is a trehalose+active structure. I think this would be classified as an extract not an isolate. (This was done with nothing but 180 proof)
I've had luck crystallizing out of AB extraction. anyhydrous methanol/ethanol work for ReX
The Isolate crystals are translucent and needle like.
also just read something on an 80/20 acetone mix with water. if anything I feel like that would clean it up the most. allowing the sugars to stay in solution with the water. while actives drop out in cold acetone.

also a large portion of the Hoffman paper. I went out and searched it. describes chromatography methods for isolation. only mentioning halogen preventing the crystalization.
if you need an isolate the chances are you're going to try vaping it. I would fill a cart and not need a crystalline isolate.
science does update from time to time. solvent rated membranes are fun.
Hoffman's experimental: https://patents.google.com/patent/US3183172A/en
I've also had crystals precip out of the alkanes after preforming A/B on extract. however I recommend finding a better NP solvent for your pulls and then use the tane to clean up
I want to try a couple things for further clean up. I would like to try sublimination as well as a Straight To Base process. I just haven't gotten around to it yet
Edited by DINKLB3RG (06/07/21 11:15 AM)
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