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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27149426 - 01/15/21 09:30 AM (3 years, 1 month ago)

1/15/2021

Small update, here are my T1 TOC and TWC chugging along.



Update to OP here: https://www.shroomery.org/forums/showflat.php/Number/27125101#27125101


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27149440 - 01/15/21 09:41 AM (3 years, 1 month ago)

Late update:


:mushdance::mushdance::mushdance::mushdance:January 14th, 2021:mushdance::mushdance::mushdance::mushdance:


Made a couple T1s and started more T0s. Mostly just wanted to get everything on MEA instead of PDA. A bit of ups and downs so I'll try to detail them separately.

T1
-GT
-AA+

T0
-Z
-CR
-PFC
-AA+


The Bad: Working in less than ideal settings; lighting was terrible and myc was quite hard to see while in SAB. Took 2-3 wedges out of T0s for T1s but I am not 100% sure I actually got any myc.

The Good: MEA plates came out looking great! Like looking through amber. Poured a full sleeve but used less than half so plates ready to go for next transfers. Of the PDA plates I pour and did not use, none of them are showing any contamination. I'll not likely use them for anything unless no other option but it is a nice reassurance that my technique hasn't completely gone to shit and contams on T0s are likely from dirty prints/mss.


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27149452 - 01/15/21 09:47 AM (3 years, 1 month ago)

Why the switch, are you having issues with your PDA?


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27149556 - 01/15/21 11:06 AM (3 years, 1 month ago)

I've just never gotten good results with it. Growth always looks super wispy, and I just wasn't happy with the mix. A little harder than I like and cloudy.


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OfflineBrownBear
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
    #27149740 - 01/15/21 01:01 PM (3 years, 1 month ago)

My plates still end up with specs of setiment in them. So my plates aren't quite as prestine as D3monic's. D3monic sure does some beautiful agar work.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27149761 - 01/15/21 01:20 PM (3 years, 1 month ago)

It very well could be operator error, but I can just never get this kind of clarity with PDA. I think it's the starch that fucks it for me.

Glare makes it looks less clear in photo than it is.

PDA is what I first started with but once I tried MEA, I know I never wanted to go back. I still keep it around just in case though.


Edited by GypsyBastard (01/15/21 01:24 PM)


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27149782 - 01/15/21 01:31 PM (3 years, 1 month ago)


Here's how I get my plates looking like glass.
Distilled water 500ml gets heated to a mild simmer, do not boil.
I add 1~g of LME to the heated distilled water and stir until the 1g LME is completely dissolved.
10g agar is then added to this mixture and the vessel is shaken to mix it up.
I load my agar vessel, this is a Pyrex 500ml media bottle.
I put polyfil into the top of the vessel and use tinfoil as a lid to prevent moisture from entering the vessel.
PC for 45min.
Once its out of the PC I remove the polyfil/tinfoil and set it up to pour.
My drip ring is sanitized with a hot soapy bath and iso wipe before being attached.
The lid gets the same treatment, sometimes I spray them down with lysol because I am a germaphobe, doing so makes me feel comfortable using them to pour.
I shake up the vessel at this point, this makes sure its all mixed evenly.
I temp my agar vessel every 10-15min. As soon as it hits 51c(125f) I pour my plates.
Doing so this way I haven't had any contam plates before actually attempting to use them.
plus they're clear as glass.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27149799 - 01/15/21 01:44 PM (3 years, 1 month ago)

Quote:

mushhead said:

Here's how I get my plates looking like glass.
Distilled water 500ml gets heated to a mild simmer, do not boil.
I add 1~g of LME to the heated distilled water and stir until the 1g LME is completely dissolved.
10g agar is then added to this mixture and the vessel is shaken to mix it up.
I load my agar vessel, this is a Pyrex 500ml media bottle.
I put polyfil into the top of the vessel and use tinfoil as a lid to prevent moisture from entering the vessel.
PC for 45min.
Once its out of the PC I remove the polyfil/tinfoil and set it up to pour.
My drip ring is sanitized with a hot soapy bath and iso wipe before being attached.
The lid gets the same treatment, sometimes I spray them down with lysol because I am a germaphobe, doing so makes me feel comfortable using them to pour.
I shake up the vessel at this point, this makes sure its all mixed evenly.
I temp my agar vessel every 10-15min. As soon as it hits 51c(125f) I pour my plates.
Doing so this way I haven't had any contam plates before actually attempting to use them.
plus they're clear as glass.





Oh wow, you only use 1g LME? Is that just for germ plates or your standard mix?


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27149805 - 01/15/21 01:46 PM (3 years, 1 month ago)

that's my standard, I have never had any other need to add higher nutes to my plates.
higher nute plates grow the same rate ime, sometimes they impede your myc because its not needing to spread because it has an abundance of nutes right where its growing.
I have also read that low nute agar is more contam resistant due to the lack of nutes required for some bacteria to survive.
However I have no evidence for that, but all my plates before I open and attempt to use them are as clean as a pane of glass just done up by Windex. 
EDIT: I don't have a flowhood just yet so all this is done in an SAB.
I fricken hate my SAB
I DON'T WANT TO FLIPPIN USE IT.


Edited by mushhead (01/15/21 01:57 PM)


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Invisiblecapntrips7
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27149816 - 01/15/21 01:54 PM (3 years, 1 month ago)

Quote:

Iambrownbear said:
Why did you take transfers from spots that are showing bacteria colonies?




I was staying as far away from the mold as possible. I've had much better luck getting away from bacteria than mold in my other plates. I suppose I could have tried to take a cut between the mold and bacteria, but I'm not great with a scalpel.


--------------------
LAGM2021


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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27149824 - 01/15/21 01:58 PM (3 years, 1 month ago)

Why don't you PC the lid and drip ring? Those GL45 lids/rings were designed for autoclave runs.

Also, I will only be using distilled water for agar from now on. 500ml distilled, 9g agar 8g LME:



--------------------



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
    #27149843 - 01/15/21 02:10 PM (3 years, 1 month ago)

:neat:
I've always used around 3% for standard mix. Usually do 1.5% for T0s but just used standard this time since they would be used for transfers as well. I did drop the agar down a bit though to soften it. about 6.5g/450ml.

Same question as bigfoots though. I'm curious about that as well.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27149845 - 01/15/21 02:12 PM (3 years, 1 month ago)

Quote:

bigfootscreepyuncl said:
Why don't you PC the lid and drip ring? Those GL45 lids/rings were designed for autoclave runs.

Also, I will only be using distilled water for agar from now on. 500ml distilled, 9g agar 8g LME:





I know the lids can survive a PC, but I am paranoid that my drip ring wont.
So I just don't PC either of them.


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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27149855 - 01/15/21 02:19 PM (3 years, 1 month ago)

Fair enough. So far my rings have done just fine. My problem has been the actual glass, of all things. I sent 2 new media bottles through the PC and they both developed hairline cracks..? I'm waiting on replacements from the distributor (free of charge), that was just really unexpected.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27149873 - 01/15/21 02:33 PM (3 years, 1 month ago)

I grabbed an amazon deal a while back for 3 500ml media bottles for like $18. I was super worried about that. Them being so cheap I was worried the manufacturing quality of the glass may be lacking. Luckily, so far anyway, I have had no issues with them. Which is good because I really doubt I would have gotten free replacements.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27149900 - 01/15/21 02:52 PM (3 years, 1 month ago)

Dude here's what you do.
Order your media bottles from amazon.
Once it's delivered tell them you didn't get it.
They'll replace your order for free no questions asked.
I did this with an order of a dozen 3 brick ecoearth coir packs.
Safe to say I wont have to buy Coir for a while now.
Also I'm sure if they cracked within a month of getting them there is a policy to have them replaced too.
Amazon caters, but I hate using them.
Most my supplies now come from local science supply outlets I use.


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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27149928 - 01/15/21 03:16 PM (3 years, 1 month ago)

$18 for 3, 500s is a steal! I just paid like $16 each for 250ml media bottles for LC. It's always confused me how a lot of times smaller items are more expensive than the bigger models lol. Now I just need some clean enough cultures to start playin with LC!

I got a black friday amazon deal for 22lb of coir for $16 and my local hardware store has the good 54qt sterilite totes on sale for $8 and 50lb bags of WBS (no corn and very little sunflower seed) for $9.

I should be going full tilt in just a couple months!


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27149945 - 01/15/21 03:31 PM (3 years, 1 month ago)

Quote:

bigfootscreepyuncl said:
$18 for 3, 500s is a steal! I just paid like $16 each for 250ml media bottles for LC. It's always confused me how a lot of times smaller items are more expensive than the bigger models lol. Now I just need some clean enough cultures to start playin with LC!

I got a black friday amazon deal for 22lb of coir for $16 and my local hardware store has the good 54qt sterilite totes on sale for $8 and 50lb bags of WBS (no corn and very little sunflower seed) for $9.

I should be going full tilt in just a couple months!




Yeah seriously... I bought x2 of the Pyrex brand 500ml bottles for ~$22 each. They're amazing but a little spendy.

Nice deal on that coir! Wish my local hardware store had it for a bit cheaper.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27149955 - 01/15/21 03:38 PM (3 years, 1 month ago)

Hell ya bigfoots! Sounds like you are stocked up. I miss living in a city that had stores useful to this hobby. We are too rural to have science or brewing or hydro supply stores but not rural enough for feed stores. If I can't find it at Ace or TSC, then it's not here. So I was ecstatic when a friend offered to give me like 2 kilos of LME for free.
Given, much of what we need can be found at those 2 places. So at least there's that.

Yeah I got lucky with that find. Think it was around christmas a year or two ago. I have 2 pyrex and 3 Cadamada and the 3 cost less that one of the pyrex.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27149959 - 01/15/21 03:39 PM (3 years, 1 month ago)


UPDATE: 1/15/21 Agar has slowed down after being dropped to grain.
Myc is now jumping to the grain, you can see it reaching for it.
Soon it shall be as swift as the roadrunner.


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