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 Josborne
Stranger
Registered: 11/18/20
Posts: 77
Last seen: 2 days, 13 hours
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 Is This Mycelium? (Agar Plate) 
#27145936 - 01/13/21 02:14 PM (1 month, 20 days ago) |
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From MS Syringe. Wondering if this is mycelium and if it is/isin't, how you are able to tell. Thanks!
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insutama
No pain no gain
 
Registered: 11/24/08
Posts: 1,110
Loc: Canada 
Last seen: 13 minutes, 46 seconds
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Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
#27146032 - 01/13/21 03:13 PM (1 month, 20 days ago) |
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yes
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Schemenhaft
Oachkatzlschwoaf
Registered: 01/14/17
Posts: 333
Loc: Right side of the big poo...
Last seen: 21 hours, 55 minutes
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Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
#27146034 - 01/13/21 03:14 PM (1 month, 20 days ago) |
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Yes it is.
Experience.
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 bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 480
Loc: Gamehenge
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Re: Is This Mycelium? (Agar Plate) [Re: Schemenhaft]
#27146409 - 01/13/21 07:23 PM (1 month, 20 days ago) |
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What was your method for inoculating the plate? I'm assuming that was from a MS syringe?
I can't get mine to germinate for shit
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  Bigfoots LAGM21  
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Josborne
Stranger
Registered: 11/18/20
Posts: 77
Last seen: 2 days, 13 hours
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Quote:
bigfootscreepyuncl said:
What was your method for inoculating the plate? I'm assuming that was from a MS syringe?
I can't get mine to germinate for shit
I inoculated a bunch of plates and only two germinated, so I feel you.
Yes I used a MS syringe. I flame sterilized the needle, waited for it to cool, and then pushed out a 1-2 drops onto the plate (all done in a SAB). Then wrap in parafilm.
I think germination results are more dependent on agar recipe. I can't remember the teks but I remember seeing a few on here made specifically for making the agar more viable for spore germination.
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Josborne
Stranger
Registered: 11/18/20
Posts: 77
Last seen: 2 days, 13 hours
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Re: Is This Mycelium? (Agar Plate) [Re: Schemenhaft]
#27146670 - 01/13/21 10:34 PM (1 month, 20 days ago) |
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Quote:
Schemenhaft said:
Yes it is.
Experience.
I opened up the plate in open air (dumb I know). Is it still worth doing transfers or did that compromise the entire plate?
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Pearl
Stranger
Registered: 05/28/20
Posts: 261
Last seen: 8 days, 2 hours
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Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
#27146996 - 01/14/21 03:35 AM (1 month, 20 days ago) |
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You can still transfer to get 100% clean.
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tinyshrimp
Stranger
Registered: 11/16/20
Posts: 31
Last seen: 2 hours, 32 minutes
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Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
#27147023 - 01/14/21 04:23 AM (1 month, 20 days ago) |
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Even if the plate got "dirty" agar is a fantastic medium to clean cultures. So yeah, do a transfer, if it gets dirty do another one. Once you have a bit of experience you will be surprised what things you can actually "save".
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HappinessStan
Fungivore
Registered: 10/10/12
Posts: 324
Loc: Worcester, UK
Last seen: 1 day, 2 hours
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Quote:
bigfootscreepyuncl said:
What was your method for inoculating the plate? I'm assuming that was from a MS syringe?
I can't get mine to germinate for shit
Try brf pucks. I had the same issues, only getting bacteria on my plates and no myc. Saw someone suggest brf pucks, tried it, and all my syringes germinated in less than 7 days. Then I just plucked some myc off with sterilised tweezers and put it to agar to clean up.
Those brf pucks have just started pinning so I'm gonna clone and print some of the best shrooms and I've put the agar plates to more grain.
Try it, it works. Hope it helps.
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Once upon a time in the land of dreams,
where the sky was silky soft and full of coloured dreams.
Deep inside a rainbow lived Happiness Stan,
in a small Victoriana charabanc.
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bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 480
Loc: Gamehenge
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Thanks for the tip, HappinessStan! It's such a simple concept that I would never have thought of myself lol. I've been thinking for the last month I'm going to just make some cakes to inoculate so I can get some clones/prints from but this will save me quite a bit of time. Cheers!
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Bigfoots LAGM21
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