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OfflineNinnyMuggins
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My Dishes Don't Look Happy...Do They?
    #27114266 - 12/29/20 03:33 PM (27 days, 22 hours ago)

Hello all, it's been quite a while and it feels good to be back in the warm embrace of the Shroomery forums :grin:

Not long ago a member here helped me out with a monoculture of PE that was doing really well, but after a while of not growing and then moving and subsequently leaving all my cultures in the car I killed everything.

I purchased 2 prints (B+ and GT) and a syringe (PE) and inoculated 10 agar dishes that I pre-poured and PC'd at 15psi for 20min on 12/20. 4 with the PE syringe, 3 with the GT and 3 with the B+

So far they don't look quite right to me but maybe you all can set me straight.

PE Dishes: (1 went bacterial, 1 has no growth of any kind)



GT Dishes:


B+ Dishes:


I'm thinking it's all looking a bit too fuzzy and weird...but I'm just a newb and can't be trusted.

I used pre-sterilized scalpel blades to scrape the spores into the dishes. I know that's not the preferred method after reading a bit, but that's what the black specs are in the GT and B+ dishes. The PE syringe had a big clumpy floater that came out and is 1 of the 3 spots that are pictured.

I had to do some hard internal work to stop kicking myself for letting everything die the way I did...ugh; I had such a good thing going and I didn't know what I had till it was gone...c'est la vie

Anyway, I would love to hear your thoughts on what's growing here. Thanks for any help!


Edited by NinnyMuggins (12/29/20 03:45 PM)


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OfflineDoctor MarioS
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Re: My Dishes Don't Look Happy...Do They? [Re: NinnyMuggins]
    #27114304 - 12/29/20 03:58 PM (27 days, 22 hours ago)

Most of those look like myc to me but are definitely worth a transfer.

The b+ dishes look kinda like some mold I ran into a few times.

Maybe this will help.

The ones that look wispy are suspect. You wanna see the myc growing uniform. If it looks like polyfil, its probably not myc.


Edited by Doctor Mario (12/29/20 04:03 PM)


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OfflineNinnyMuggins
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Re: My Dishes Don't Look Happy...Do They? [Re: Doctor Mario]
    #27115482 - 12/30/20 07:53 AM (27 days, 6 hours ago)

Thanks so much for the reply!
You're thread is very helpful, I'm going to let these grow out a bit more to see how they developed before transferring. I'll be posting back here with more pics as things come along.

Thanks again!


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OfflineNinnyMuggins
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Re: My Dishes Don't Look Happy...Do They? [Re: NinnyMuggins]
    #27146092 - 01/13/21 04:57 PM (12 days, 21 hours ago)

Thought I would make an update.

Dishes are all contam free.

The GT's are not really doing much of anything, just 1 little spot where there is real growth.

PE 3's all seemed to just stop growing for some reason.



I'd like y'alls opinion.

I was planning on just dumping these dishes into grain jars, seeing which ones do best and then isolating from there since I still have the original dishes and marked them for isolation purposes. But would any of you try to isolate the strongest looking mycelium first for another round of dishes before going to grain?
I'm leaning toward just inoculating the jars but wanted to get some more input first.

Thanks for any help!


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OfflineCrackatoaS
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Re: My Dishes Don't Look Happy...Do They? [Re: NinnyMuggins]
    #27146574 - 01/13/21 10:29 PM (12 days, 15 hours ago)

I would def take some transfers first. The picture on the left has some beautiful places to snag on all the plates. There's a few I wouldnt put to grain but thats just me. If you have a great one you'll have a plate from tub you like.


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OfflineNinnyMuggins
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Re: My Dishes Don't Look Happy...Do They? [Re: Crackatoa]
    #27147665 - 01/14/21 02:29 PM (11 days, 23 hours ago)

Quote:

Crackatoa said:
I would def take some transfers first. The picture on the left has some beautiful places to snag on all the plates. There's a few I wouldnt put to grain but thats just me. If you have a great one you'll have a plate from tub you like.




Thanks for the reply!

Is it the smaller growths that you wouldn't put to grain?

What sections would you want to clone first?  I need to look up how to pinpoint strong mycelium.


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OfflineCrackatoaS
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Re: My Dishes Don't Look Happy...Do They? [Re: NinnyMuggins]
    #27147717 - 01/14/21 03:01 PM (11 days, 23 hours ago)

No problem, check out this thread. It's older but amazing. I believe everybody that does agar should read this, if you have any questions after reading this, just ask :thumbup:
https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998


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