Home | Community | Message Board


Edabea
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon Agar, Brown Rice Flour, Parafilm

Jump to first unread post. Pages: 1
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 64
Last seen: 21 hours, 12 minutes
Is This Mycelium? (Agar Plate)
    #27145936 - 01/13/21 03:14 PM (13 days, 17 hours ago)

From MS Syringe. Wondering if this is mycelium and if it is/isin't, how you are able to tell. Thanks!




Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineinsutama
No pain no gain
Male User Gallery


Registered: 11/24/08
Posts: 974
Loc: Canada
Last seen: 13 days, 10 hours
Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
    #27146032 - 01/13/21 04:13 PM (13 days, 16 hours ago)

yes


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineSchemenhaft
Oachkatzlschwoaf
Male User Gallery


Registered: 01/14/17
Posts: 280
Loc: Right side of the big poo...
Last seen: 4 hours, 48 minutes
Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
    #27146034 - 01/13/21 04:14 PM (13 days, 16 hours ago)

Yes it is.
Experience.


--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 301
Loc: Gamehenge
Re: Is This Mycelium? (Agar Plate) [Re: Schemenhaft]
    #27146409 - 01/13/21 08:23 PM (13 days, 12 hours ago)

What was your method for inoculating the plate? I'm assuming that was from a MS syringe?

I can't get mine to germinate for shit


--------------------
Bigfoots LAGM21


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 64
Last seen: 21 hours, 12 minutes
Re: Is This Mycelium? (Agar Plate) [Re: bigfootscreepyuncl]
    #27146667 - 01/13/21 11:32 PM (13 days, 9 hours ago)

Quote:

bigfootscreepyuncl said:
What was your method for inoculating the plate? I'm assuming that was from a MS syringe?

I can't get mine to germinate for shit




I inoculated a bunch of plates and only two germinated, so I feel you.

Yes I used a MS syringe. I flame sterilized the needle, waited for it to cool, and then pushed out a 1-2 drops onto the plate (all done in a SAB). Then wrap in parafilm.

I think germination results are more dependent on agar recipe. I can't remember the teks but I remember seeing a few on here made specifically for making the agar more viable for spore germination.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 64
Last seen: 21 hours, 12 minutes
Re: Is This Mycelium? (Agar Plate) [Re: Schemenhaft]
    #27146670 - 01/13/21 11:34 PM (13 days, 9 hours ago)

Quote:

Schemenhaft said:
Yes it is.
Experience.




I opened up the plate in open air (dumb I know). Is it still worth doing transfers or did that compromise the entire plate?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflinePearl
Stranger


Registered: 05/28/20
Posts: 235
Last seen: 19 hours, 16 minutes
Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
    #27146996 - 01/14/21 04:35 AM (13 days, 4 hours ago)

You can still transfer to get 100% clean.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinetinyshrimp
Stranger
 User Gallery

Registered: 11/16/20
Posts: 17
Last seen: 39 minutes, 13 seconds
Re: Is This Mycelium? (Agar Plate) [Re: Josborne]
    #27147023 - 01/14/21 05:23 AM (13 days, 3 hours ago)

Even if the plate got "dirty" agar is a fantastic medium to clean cultures. So yeah, do a transfer, if it gets dirty do another one. Once you have a bit of experience you will be surprised what things you can actually "save".


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHappinessStan
Fungivore
Male User Gallery


Registered: 10/10/12
Posts: 244
Loc: Worcester, UK
Last seen: 1 hour, 28 minutes
Re: Is This Mycelium? (Agar Plate) [Re: bigfootscreepyuncl]
    #27147032 - 01/14/21 05:37 AM (13 days, 3 hours ago)

Quote:

bigfootscreepyuncl said:
What was your method for inoculating the plate? I'm assuming that was from a MS syringe?

I can't get mine to germinate for shit




Try brf pucks. I had the same issues, only getting bacteria on my plates and no myc. Saw someone suggest brf pucks, tried it, and all my syringes germinated in less than 7 days. Then I just plucked some myc off with sterilised tweezers and put it to agar to clean up.
Those brf pucks have just started pinning so I'm gonna clone and print some of the best shrooms and I've put the agar plates to more grain.
Try it, it works. Hope it helps.


--------------------
Once upon a time in the land of dreams,
where the sky was silky soft and full of coloured dreams.
Deep inside a rainbow lived Happiness Stan,
in a small Victoriana charabanc.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 301
Loc: Gamehenge
Re: Is This Mycelium? (Agar Plate) [Re: HappinessStan]
    #27147052 - 01/14/21 05:57 AM (13 days, 2 hours ago)

Thanks for the tip, HappinessStan! It's such a simple concept that I would never have thought of myself lol. I've been thinking for the last month I'm going to just make some cakes to inoculate so I can get some clones/prints from but this will save me quite a bit of time. Cheers!


--------------------
Bigfoots LAGM21


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon Agar, Brown Rice Flour, Parafilm

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 6,514 3 12/04/99 02:35 AM
by Anonymous
* Whats with my agar plates? Frankenstein 5,121 6 04/08/02 05:13 PM
by Seuss
* How many mycelium syringes from one agar plate? BBShrizz 2,644 3 05/10/01 04:10 AM
by ISaToriBluEI
* Re: agar plate question Psilowarrior 1,739 2 12/10/99 04:09 PM
by rebelmoon
* Spore Syringe on Agar plates Heruuka 1,840 2 09/16/02 08:31 AM
by Anno
* Re: Multispore Mycelium Agar Culturing TEK sutec 1,774 1 12/03/99 12:55 PM
by Anonymous
* Benefits of Agar? celsius 6,419 10 12/01/19 02:36 AM
by wildernessjunkie
* transferring mycelium wikedanjel 3,355 13 01/10/03 05:54 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
171 topic views. 29 members, 117 guests and 14 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
DoubleBlind - Learn to Grow Mushrooms
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.05 seconds spending 0.012 seconds on 16 queries.