Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
OfflineKyngwhatt
presbyter
Other

Registered: 02/05/20
Posts: 272
Loc: The pharcyde Flag
Last seen: 2 years, 11 months
troubleshoot my slant failure
    #27140027 - 01/10/21 03:12 PM (3 years, 1 month ago)

This was my first time using slants and it failed.
I have some sediment in the bottom that wasn't there when i laid them to cool.
I have the cap a little loose and wrapped the gap with 2 wraps of parafilm maybe 3.
I put them in a plastic sandwich bag to colonize like i read in a book but it's like they suffocated and died. are you to leave them out and after they colonize you put them in a bag and then in the fridge.

Do you see anything i clearly did wrong or would you care to explain how you do it?



Thanks fam


--------------------


Whatt


Edited by Kyngwhatt (01/10/21 03:17 PM)


Extras: Filter Print Post Top
InvisibleButtFace
Registered: 08/06/08
Posts: 1,552
Re: troubleshoot my slant failure *DELETED* [Re: Kyngwhatt]
    #27140088 - 01/10/21 03:52 PM (3 years, 1 month ago)

Post deleted by TieACable2aTree

Reason for deletion: f


Edited by ButtFace (01/10/21 04:07 PM)


Extras: Filter Print Post Top
OfflineKyngwhatt
presbyter
Other

Registered: 02/05/20
Posts: 272
Loc: The pharcyde Flag
Last seen: 2 years, 11 months
Re: troubleshoot my slant failure [Re: ButtFace]
    #27140145 - 01/10/21 04:26 PM (3 years, 1 month ago)

thanks for the reply and the link!
Quote:

RogerRabbit said:
I also make up the FP agar about 20% weaker than called for in the instructions.  This takes care of most of the sediment and the slants solidify normally.
RR


is a really good tip.


i would have thought a little sediment would still allow the myc to colonize it a little bit. If you look at the 3rd pic they didn't even try to colonize the slant. I know i used a really small wedge from the plate but that shouldn't matter right? I guess i'm just going to have to try again with less parafilm and a larger wedge.
UNLESS ! my scalpel was too hot for the size of the wedge. :sonofawebitch:


--------------------


Whatt


Extras: Filter Print Post Top
InvisibleButtFace
Registered: 08/06/08
Posts: 1,552
Re: troubleshoot my slant failure *DELETED* [Re: Kyngwhatt]
    #27140167 - 01/10/21 04:44 PM (3 years, 1 month ago)

Post deleted by TieACable2aTree

Reason for deletion: delete


Edited by ButtFace (01/10/21 05:03 PM)


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: troubleshoot my slant failure [Re: ButtFace]
    #27140197 - 01/10/21 05:01 PM (3 years, 1 month ago)

i started venting my caps on my slants with sfd👍👍👍


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
InvisibleButtFace
Registered: 08/06/08
Posts: 1,552
Re: troubleshoot my slant failure *DELETED* [Re: jcm4620]
    #27140201 - 01/10/21 05:04 PM (3 years, 1 month ago)

Post deleted by TieACable2aTree

Reason for deletion: delete


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: troubleshoot my slant failure [Re: ButtFace]
    #27140400 - 01/10/21 06:37 PM (3 years, 1 month ago)

nope but i wrap it anyway outta habit they store perfectly good👍


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,941
Loc: 7b
Last seen: 15 hours, 53 minutes
Trusted Cultivator
Re: troubleshoot my slant failure [Re: jcm4620]
    #27140524 - 01/10/21 07:30 PM (3 years, 1 month ago)

How long is perfectly good?
I gave up on culture slants entirely.
I've had better luck reviving 3 year old myc from grain jars kept at fluctuating yearly temps.

Maybe I should re visit them SFD on the lid.


Extras: Filter Print Post Top
InvisibleEZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
Re: troubleshoot my slant failure [Re: bw86]
    #27140556 - 01/10/21 07:41 PM (3 years, 1 month ago)

Quote:

bw86 said:
How long is perfectly good?




Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: troubleshoot my slant failure [Re: EZPZyaBZ]
    #27140801 - 01/10/21 09:10 PM (3 years, 1 month ago)

i have just started venting like that about 14-16 months ago and i love it and have many cultures in my fridge in slants that are a year old and still perfectly viable. i try to always make sure i transfer to fresh slants at least once a year


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (01/10/21 09:12 PM)


Extras: Filter Print Post Top
OfflineTigerStrike
carpophore locator
 User Gallery


Registered: 12/22/20
Posts: 315
Last seen: 1 year, 9 months
Re: troubleshoot my slant failure [Re: bw86]
    #27140938 - 01/10/21 10:11 PM (3 years, 1 month ago)

I will assume you did MEA with a pinch of brewers yeast? All my plates look like that with that recipe. Eh?


The shit at the bottom is looks to be undisolved precipitate.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Rookie needs troubleshooting help Chemical_Smile 1,922 13 05/15/16 10:58 AM
by Captcrunch90
* Cubensis Pinning Strategy & Troubleshooting
( 1 2 3 4 5 6 7 8 9 all )
Primal Call 86,285 173 09/25/23 01:53 PM
by Benson
* My G2G has been very successful, despite my casing failure... Maverick 514 7 08/15/08 11:41 AM
by Maverick
* malt/dextrose sediment in LC with stir plate davesj1 2,462 15 07/03/06 10:07 PM
by Omnicracker
* I keep filtering the LC and sediment keeps showing up! saltyd 1,028 5 07/09/11 11:20 PM
by scatmanrav
* From What I've read about malt LC sediment... kiddd038 883 0 09/17/08 09:51 PM
by kiddd038
* Mephisto's Master Slant Tek
( 1 2 3 4 5 6 7 all )
Mephistophelian 38,411 124 06/10/19 08:54 PM
by Mephistophelian
* Master Slants & Pan. Cyans. - Q's Shot.In.Orbit 991 4 12/25/10 06:51 PM
by jokefox

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
279 topic views. 7 members, 113 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.005 seconds on 12 queries.