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OfflineZsuzsi
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Registered: 03/05/19
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Last seen: 6 months, 15 days
Potential contam in my tub ? mycogone ?
    #27140357 - 01/10/21 06:24 PM (3 years, 18 days ago)

Hi all,

I have a tub that was spawned to bulk 12 days ago (agar T2, WBS, 1:2, pure coir). Here is a global view of the tub:



See the brown part at the upper right ? it was covered with a few drops of what looked like metabolites. I quickly used a paper towel to remove those drops. Here is a closeup:



Even bigger closeup:



What is this ? the only thing close I found searching the forum is, perhaps, mycogone ?

Also, how does the tub look in general ? surface was colonized pretty quickly but it has slowed down a bit in the colonization of the interior thus far. I'm a bit worried as it's already been 11 days and no signs of pins.

I also have a second tub that's similar but without the issue outlined above.

Thanks in advance !


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OfflineBoatloaf
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27140927 - 01/10/21 10:02 PM (3 years, 17 days ago)

Hm... my first thought was metabolite... but the rest of the tub looks really fuzzy and kinda suspect to me


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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: Boatloaf]
    #27140952 - 01/10/21 10:23 PM (3 years, 17 days ago)

For what it's worth, no suspect smells there (smells mushroomy and that's it). Was similar for the spawn jars. And yes, there was a big drop of metabolites there before I mostly wiped it off.

The growth was really tomentose throughout (it's MS).


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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27142423 - 01/11/21 05:12 PM (3 years, 17 days ago)

Anyone else ? does the surface look suspicious to you too ?


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OfflineDigDug
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27142585 - 01/11/21 07:26 PM (3 years, 16 days ago)

It looks a little suspect to me, but I've had tubs that looked like this turn out just fine.  Not saying it's 100% clean, but I'd let it grow and see what comes of it.


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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: DigDug]
    #27142621 - 01/11/21 07:53 PM (3 years, 16 days ago)

Thanks. I'm a tad worried because the interior colonization looks, if not stalled, at least much slower than before.

Considering putting the lid reversed (it has a pretty tight seal) to try and increase FAE / evap a bit, but at the same time I'm worried messing too much with it will end in failure.


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OfflineNichrome
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27142828 - 01/11/21 10:41 PM (3 years, 16 days ago)

Whether or not it is a concern should become evident soon enough. What it is at this point is "small", but depending on how fast it grows or doesn't should be the determining factor of toss or not toss.

It looks like a spot where a condensation droplet of some kind deposits debris/minerals etc. without better pictures it will be hard to get a definitive answer.

It is possible it will eventually grow tentacles and envelop you with protienous goo after seducing you with mind altering pheromones (the good ones). Eventually you will erupt in a glorious spectacle of writhing piles of whatever that thing is's progeny. This is not likely but surely physically possible.

One thing I can say is that is an awful lot of fluffy mycelium without any sign of pinning which is often climatically induced or the colony is fighting something like bacteria or a virus (fungi get viruses too) or possibly mold or microscopic nematodes.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: Nichrome]
    #27143080 - 01/12/21 02:16 AM (3 years, 16 days ago)

Hi,

Quote:

Nichrome said:
Whether or not it is a concern should become evident soon enough. What it is at this point is "small", but depending on how fast it grows or doesn't should be the determining factor of toss or not toss.




It started with a drop of water slightly larger than the others, then turned yellow/golden (metabolites), then grew slightly larger the day after, then stopped growing larger. That was ~5 days ago, so I guess that's a good sign ?

Right now I'm more worried about the slow development of my myc *inside* the substrate - I got no liner so I can see it. There's myc almost everywhere but it's thin, and consolidated / solid white only in places. I marked those places with a marker and while they're still growing, they're doing so extremely slowly. Perhaps I don't have enough FAE ? or my substrate was too wet ? tested it for field capacity, though, and I opted to err on the drier side just in case.

Quote:

Nichrome said:
It is possible it will eventually grow tentacles and envelop you with protienous goo after seducing you with mind altering pheromones (the good ones). Eventually you will erupt in a glorious spectacle of writhing piles of whatever that thing is's progeny. This is not likely but surely physically possible.




God, I wish :blush:

I really dig your profile pic, by the way.


Edited by Zsuzsi (01/12/21 02:16 AM)


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InvisibleRafiikii
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27143138 - 01/12/21 03:45 AM (3 years, 16 days ago)

Slightly bacterial if I had to guess


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."



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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: Rafiikii]
    #27143155 - 01/12/21 04:26 AM (3 years, 16 days ago)

Thanks, Rafiikii. Still praying for at least one flush.

My second tub doesn't have any metabolites, but the surface looks extremely similar.


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InvisibleRafiikii
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Re: Potential contam in my tub ? mycogone ? [Re: Zsuzsi]
    #27144056 - 01/12/21 02:59 PM (3 years, 16 days ago)

Should still pop some fruits, wouldn’t worry about it much.


--------------------
"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."



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OfflineZsuzsi
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Re: Potential contam in my tub ? mycogone ? [Re: Rafiikii]
    #27221436 - 02/23/21 02:01 AM (2 years, 10 months ago)

Update in case anyone cares: it's been two months now. The weird blob I showed in my post was followed (about two weeks later from that picture) by similar blobs on many places, which made me think the tub was a likely toss. My second monotub also went bacterial I think, although less so and without those blobs. So my hopes were pretty low.

About one week after that, though, healthy myc started to cover all the blobs in the first tub, almost like it was winning the battle; so my hopes went back up, and furthermore the entirety of the substrate (even inside I mean) consolidated a lot, also suggesting it was fighting and winning.

But then not much in terms of evolution for 3 more weeks, so I figured I should probably shred it into a flower pot and put it outside and consider this a loss. But then I figured: why not wait after all ? there's no mold or trich thus far, after all.

And then today, what do I see in that first tub ? a single pin (looks a bit weird but it's there) ! finally. Maybe it's still a loss and there won't be any other pins but now my hopes are back up.

I'll be sure to post pictures if there's actually a flush - and will likely post pictures anyway of my spawn jars as post mortem / to get feedback on if I should've been able to detect bacteria visually and didn't.


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