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Kyngwhatt
presbyter


Registered: 02/05/20
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troubleshoot my slant failure
#27140027 - 01/10/21 03:12 PM (3 years, 1 month ago) |
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Whatt
Edited by Kyngwhatt (01/10/21 03:17 PM)
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ButtFace
Registered: 08/06/08
Posts: 1,552
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Re: troubleshoot my slant failure *DELETED* [Re: Kyngwhatt]
#27140088 - 01/10/21 03:52 PM (3 years, 1 month ago) |
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Post deleted by TieACable2aTree
Reason for deletion: f
Edited by ButtFace (01/10/21 04:07 PM)
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Kyngwhatt
presbyter


Registered: 02/05/20
Posts: 272
Loc: The pharcyde
Last seen: 2 years, 11 months
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Re: troubleshoot my slant failure [Re: ButtFace]
#27140145 - 01/10/21 04:26 PM (3 years, 1 month ago) |
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thanks for the reply and the link!
Quote:
RogerRabbit said: I also make up the FP agar about 20% weaker than called for in the instructions. This takes care of most of the sediment and the slants solidify normally. RR
is a really good tip.
i would have thought a little sediment would still allow the myc to colonize it a little bit. If you look at the 3rd pic they didn't even try to colonize the slant. I know i used a really small wedge from the plate but that shouldn't matter right? I guess i'm just going to have to try again with less parafilm and a larger wedge. UNLESS ! my scalpel was too hot for the size of the wedge.
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Whatt
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ButtFace
Registered: 08/06/08
Posts: 1,552
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Re: troubleshoot my slant failure *DELETED* [Re: Kyngwhatt]
#27140167 - 01/10/21 04:44 PM (3 years, 1 month ago) |
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Post deleted by TieACable2aTree
Reason for deletion: delete
Edited by ButtFace (01/10/21 05:03 PM)
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jcm4620
Stranger


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Re: troubleshoot my slant failure [Re: ButtFace]
#27140197 - 01/10/21 05:01 PM (3 years, 1 month ago) |
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i started venting my caps on my slants with sfd👍👍👍
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ButtFace
Registered: 08/06/08
Posts: 1,552
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Re: troubleshoot my slant failure *DELETED* [Re: jcm4620]
#27140201 - 01/10/21 05:04 PM (3 years, 1 month ago) |
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Post deleted by TieACable2aTree
Reason for deletion: delete
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jcm4620
Stranger


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Re: troubleshoot my slant failure [Re: ButtFace]
#27140400 - 01/10/21 06:37 PM (3 years, 1 month ago) |
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nope but i wrap it anyway outta habit they store perfectly good👍
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bw86
Doesn't play well with others


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Re: troubleshoot my slant failure [Re: jcm4620]
#27140524 - 01/10/21 07:30 PM (3 years, 1 month ago) |
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How long is perfectly good? I gave up on culture slants entirely. I've had better luck reviving 3 year old myc from grain jars kept at fluctuating yearly temps.
Maybe I should re visit them SFD on the lid.
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EZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
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Re: troubleshoot my slant failure [Re: bw86]
#27140556 - 01/10/21 07:41 PM (3 years, 1 month ago) |
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Quote:
bw86 said: How long is perfectly good?
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jcm4620
Stranger


Registered: 05/26/19
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Re: troubleshoot my slant failure [Re: EZPZyaBZ]
#27140801 - 01/10/21 09:10 PM (3 years, 1 month ago) |
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i have just started venting like that about 14-16 months ago and i love it and have many cultures in my fridge in slants that are a year old and still perfectly viable. i try to always make sure i transfer to fresh slants at least once a year
Edited by jcm4620 (01/10/21 09:12 PM)
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TigerStrike
carpophore locator



Registered: 12/22/20
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Re: troubleshoot my slant failure [Re: bw86]
#27140938 - 01/10/21 10:11 PM (3 years, 1 month ago) |
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I will assume you did MEA with a pinch of brewers yeast? All my plates look like that with that recipe. Eh?
The shit at the bottom is looks to be undisolved precipitate.
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