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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 5 days, 15 hours
Transparent mycelium slowly fills in on agar
    #27137756 - 01/09/21 02:17 PM (3 years, 19 days ago)



This particular myc above started off with a transparent edge or border that slowly fills in with the mycelium. I put some extra coloring in this agar batch but I cannot tell if this is some contamination or the coloring interefers with the myc. It looks like its struggling. For example, the arrow pointing at (1) shows a section of the mycelium that haas had healthy growth from the start.



Same here. Mostly a border of what started out as transparent mycelium which later filled up (It will fill more until it's 100% white) and a section (2) that started healthy from the beginning.

Anyone dealt with something like this before?

recipe: 500 ml water with 10g agar/7g LME


Edited by Phony Phone (01/09/21 02:18 PM)


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Transparent mycelium slowly fills in on agar [Re: Phony Phone]
    #27137827 - 01/09/21 02:56 PM (3 years, 19 days ago)

Honestly all I see is a shadow dick in picture one.

What is your question again?


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InvisibleJosex
#cheat_code
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Registered: 11/13/15
Posts: 8,995
Loc: Flag
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Re: Transparent mycelium slowly fills in on agar [Re: Sockadin]
    #27137863 - 01/09/21 03:17 PM (3 years, 19 days ago)

Quote:

Sockadin said:
Honestly all I see is a shadow dick in picture one.

What is your question again?




:lolsy:

I swear I thought the same.

Those plates are probably fine, they need a bit more work.


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OfflineProfessor X
School for the Gifted
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Registered: 04/18/19
Posts: 2,719
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Re: Transparent mycelium slowly fills in on agar [Re: Josex]
    #27137911 - 01/09/21 03:40 PM (3 years, 19 days ago)

Looks like a healthy hell of a bunch of strains.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 5 days, 15 hours
Re: Transparent mycelium slowly fills in on agar [Re: Sockadin]
    #27137961 - 01/09/21 03:58 PM (3 years, 19 days ago)

Alright, monitoring then. I take it you havent experienced such growth before?



Quote:

Sockadin said:
Honestly all I see is a shadow dick in picture one.





contams keep getting weirder and weirder


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OfflineProfessor X
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Registered: 04/18/19
Posts: 2,719
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Re: Transparent mycelium slowly fills in on agar [Re: Phony Phone]
    #27137982 - 01/09/21 04:04 PM (3 years, 19 days ago)

My AA+ looked like that after 2nd transfer. After the 3rd it was very uniform with a lot less sectors. 2 different donors to 30 plates each. I now have 60 copies of very uniform fast colonizing AA+ I have tested and fruit nicely in my myc fridge. I have in the ballpark of 100 of my ksss mono as well.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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Offlineverytastycheese
Tripmaster
Registered: 11/11/14
Posts: 101
Last seen: 2 years, 11 months
Re: Transparent mycelium slowly fills in on agar [Re: Professor X]
    #27138538 - 01/09/21 09:18 PM (3 years, 19 days ago)

Quote:

Professor X said:
My AA+ looked like that after 2nd transfer. After the 3rd it was very uniform with a lot less sectors. 2 different donors to 30 plates each. I now have 60 copies of very uniform fast colonizing AA+ I have tested and fruit nicely in my myc fridge. I have in the ballpark of 100 of my ksss mono as well.




So many plates! What's your process from there?

I've been making 2 transfers and 8 master jars per healthy plate. G2G x10 = 80 jars = 20 bins per plate. What do you need 60 for?!


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