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OfflineGermanShaman
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what concentration of Honey or LME to use for LC
    #27136881 - 01/09/21 06:14 AM (3 years, 1 month ago)

What is a good concentration to use as a LC?  I read that 4% was good, that is 4g of Honey or LME to 100g water.

But I have also seen that some people are suggesting 1g of honey/ LME to 100g water.  In some cases I have even seen weaker concentrations.

(Please dont suggest that I try various concentrations to see which one works. It takes a fair bit of time to do these experiments. I just want to find a nice concentration that will work so that I can get on with growing rather than having to spend time figuring out what to do in order to be growing. Lets learn from each other and experts who have already been down the path. That is one of the reasons I am here...)


Many thanks good folks.


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Offlinenogoodnamesleft
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27136915 - 01/09/21 06:53 AM (3 years, 1 month ago)

Run 4% if you want it to grow aggressively


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Invisiblecoversall
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Re: what concentration of Honey or LME to use for LC [Re: nogoodnamesleft]
    #27136916 - 01/09/21 06:54 AM (3 years, 1 month ago)

I've had good success with pans at 1g LME to 500ml of water.


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InvisibleJosex
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Re: what concentration of Honey or LME to use for LC [Re: coversall]
    #27136929 - 01/09/21 07:07 AM (3 years, 1 month ago)

Quote:

coversall said:
I've had good success with pans at 1g LME to 500ml of water.




That's the ratio I use too (0,2%) and it's good for cubes as well. The broth will be very clear which is important and at that ratio there's more nutrition in the broth than the myc will use, some people go as low as 0.1% with great success.
A colony needs very little energy to colonize an aqueous media.


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OfflineSold Out Online
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Re: what concentration of Honey or LME to use for LC [Re: coversall]
    #27136932 - 01/09/21 07:07 AM (3 years, 1 month ago)

This is what 0.2% LME looks like.



This is what a 50/50 dilution of rye grain soak water looks like.


The GSW may have been faster, but the LME is clear and easier to determine whether something may be amiss in mycelium town.


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Offlinenogoodnamesleft
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Re: what concentration of Honey or LME to use for LC [Re: Sold Out Online]
    #27137135 - 01/09/21 09:05 AM (3 years, 1 month ago)

At the fraction of % ratios the growth in my LC is painfully slow.

Are you incubating the LC


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OfflineGermanShaman
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Re: what concentration of Honey or LME to use for LC [Re: nogoodnamesleft]
    #27138065 - 01/09/21 04:56 PM (3 years, 1 month ago)

0.2%... wow that is really low concentration.

I think I will try a few and see what happens. I will certainly give that low concentration a try.

I certainly want fast aggressive growth.

One of the issues I am facing is blue trich on the surface, so I have to chuck out the whole jar.  Wasted time.


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OfflineGermanShaman
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27138067 - 01/09/21 04:57 PM (3 years, 1 month ago)

also what temp do you guys keep your LC at during inoculation phase?

I keep mine around 21-23 Deg C.


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OfflineProfessor X
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27138071 - 01/09/21 04:59 PM (3 years, 1 month ago)

I run 2% honey. It is far more forgiving if you PC 20 minutes instead of 15...


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InvisibleJosex
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27138091 - 01/09/21 05:14 PM (3 years, 1 month ago)

Quote:

0.2%... wow that is really low concentration.




Don't take me wrong I don't wanna sound like a dick, but it really doesn't sound like you have a good point of reference to say this. It is really not a low concentration, really more than enough.

This puts in perspective the 4% strength you were doing. Twice as much nutrition than a standard agar recipe and 20 times more than what you need for an LC, that's out of the charts nuts and does nothing but cloud the hell out of the broth.

Also, honey becomes sugar after PC'ing, sugar based LC's are not recommended. LME or grain water are superior, which answers your concern below.

Quote:

I certainly want fast aggressive growth.



These are examples of LME LC's at 0,2%, fast aggressive growth and lots of mycelial mass:

Following some simple tricks, they can be done in as little as 7 days from biopsy (see first link in my sig).


Quote:

GermanShaman said:
also what temp do you guys keep your LC at during inoculation phase?

I keep mine around 21-23 Deg C.



Room temp is fine, 21-23 is perfect.


Edited by Josex (01/09/21 05:21 PM)


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InvisibleBuddaking
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Re: what concentration of Honey or LME to use for LC [Re: Josex]
    #27138432 - 01/09/21 08:28 PM (3 years, 1 month ago)

Yep 1 gram lme to 600 ML for me. Nice & clear. I recently tried GWLC & upping my nutes. Going back to old faithfull


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OfflinePitcherCrab
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Re: what concentration of Honey or LME to use for LC [Re: coversall] * 1
    #27138734 - 01/09/21 11:01 PM (3 years, 1 month ago)

Quote:

coversall said:
I've had good success with pans at 1g LME to 500ml of water.




This is what I use as well.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineSold Out Online
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Re: what concentration of Honey or LME to use for LC [Re: nogoodnamesleft]
    #27139073 - 01/10/21 05:46 AM (3 years, 1 month ago)

Quote:

nogoodnamesleft said:
At the fraction of % ratios the growth in my LC is painfully slow.

Are you incubating the LC




I keep the room they’re in at 22C.


--------------------


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Clean Spawn checklist for new growers


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Invisiblecoversall
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Re: what concentration of Honey or LME to use for LC [Re: Josex]
    #27139079 - 01/10/21 05:52 AM (3 years, 1 month ago)

Room temp worked for me. Growth was fast enough. Broth was clear. At the moment I have no reason to consider more than 1g LME to 500ml.

Quote:

Josex said:
Quote:

coversall said:
I've had good success with pans at 1g LME to 500ml of water.




That's the ratio I use too (0,2%) and it's good for cubes as well. The broth will be very clear which is important and at that ratio there's more nutrition in the broth than the myc will use, some people go as low as 0.1% with great success.
A colony needs very little energy to colonize an aqueous media.




I got it from reading your tek :wink:


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..:: How I Panaeolus. From Agar to Tea ::..


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OfflinePitcherCrab
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Re: what concentration of Honey or LME to use for LC [Re: coversall]
    #27139192 - 01/10/21 08:29 AM (3 years, 1 month ago)

I've had mine in a cold room ~50F and while they do grow it's way better at room temperature (72F).


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineGermanShaman
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Re: what concentration of Honey or LME to use for LC [Re: Josex]
    #27142282 - 01/11/21 03:48 PM (3 years, 1 month ago)

Thanks.. No offence taken.  I was using 4% as that is the concentration used in the Psilocybin Growing bible!!!

I am trying some at 1% and 2% honey to see if that works.

Will try some at 0.2% LME as well now while I am at it.

I mean, I did not pick 4% as a random number, I thought I had a reliable source of info for it.....


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OfflinePitcherCrab
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27142287 - 01/11/21 03:52 PM (3 years, 1 month ago)

4% is what Blue Helix uses and he’s a legend with his LC work... its just about what works for you. I decided on using 1g LME to 500ml water because I read somewhere lower sugar helps reduce contamination. I don’t know how true that is, but I’ve stuck with it as it works perfectly.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineGermanShaman
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Re: what concentration of Honey or LME to use for LC [Re: PitcherCrab] * 1
    #27144263 - 01/12/21 05:35 PM (3 years, 1 month ago)

at 4% I have had problems with visible Trich blue mould on the surface.

I'll give the 0.2% LME concentration a try and see if that is any better.
I also have a load of jars sitting at 1-2% of Honey solution.

I assume that once the mycelium is growing the LC can be kept in a cool place for ages and ages, perhaps as long as a year?


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InvisibleJosex
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Re: what concentration of Honey or LME to use for LC [Re: GermanShaman]
    #27144269 - 01/12/21 05:41 PM (3 years, 1 month ago)

Yea I think you'll like 0,2%.

A 100% colonized LC has quite a bit of shelf life at room temp. I've used 2+ month old LC's without issues. After that they start to deteriorate and lose vigor. But it isn't such a good idea to use old LC's because it's so easy to make a fresh one.


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OfflineA.k.aM
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Re: what concentration of Honey or LME to use for LC [Re: Josex]
    #27144281 - 01/12/21 05:50 PM (3 years, 1 month ago)

It prob depends on the culture a lot but ive used lcs that were around 8 months and a year old and had sat at room temp the whole time which was over 100f half the time.


They definitely weren’t as quick as when they were new but still worked.


Like he said though lc is so fast I’d just make a new one unless it was some super genetics that were gone except the old lc.


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