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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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OfflineGermanShaman
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Registered: 01/05/18
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Microwave sterilization of Pertri dishes
    #27135647 - 01/08/21 04:09 PM (3 years, 20 days ago)

I use glass petri dishes for my agar work and was wondering if anyone here microwave sterilizes them before pouring agar into them.

At the moment I wipe with 70% alcohol before placing into the SAB and then pouring my agar mixture into them.  I still get issues with blue mould and am looking for ways to reduce this.

Of course this can only be done with the glass dishes and not the plastic ones.

thanks


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OfflineHappinessStan
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Registered: 10/10/12
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Re: Microwave sterilization of Pertri dishes [Re: GermanShaman]
    #27135651 - 01/08/21 04:11 PM (3 years, 20 days ago)

Pc them?


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OfflineMarxcelium
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Re: Microwave sterilization of Pertri dishes [Re: GermanShaman]
    #27135668 - 01/08/21 04:16 PM (3 years, 20 days ago)

I've never used it for mycology, but I know you can sterilize glasswear with a couple minutes in the microwave.  I used to use it to sterilize canning jars here and there.  I was always told you gotta wet them first though cause the microwave confers heat by causing the water molecules to vibrate rapidly.


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OfflineAlsetAlokin
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Registered: 07/30/20
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Re: Microwave sterilization of Pertri dishes [Re: Marxcelium]
    #27135685 - 01/08/21 04:20 PM (3 years, 20 days ago)

How do you plan on getting those "sterile" plates to the SAB from the microwave?

I've seen people wrap in foil and oven at 250 for an hour or so, but my searching led to no results.

Hope someone with more experience chimes in for you.


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
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Loc: Gamehenge
Re: Microwave sterilization of Pertri dishes [Re: AlsetAlokin]
    #27135772 - 01/08/21 04:52 PM (3 years, 20 days ago)

I think bod calls it 'dry heat sterilization' when done in the oven quoted from here

I've never heard of using the microwave for sterilization


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OfflineGermanShaman
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Registered: 01/05/18
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Re: Microwave sterilization of Pertri dishes [Re: bigfootscreepyuncl]
    #27135824 - 01/08/21 05:17 PM (3 years, 20 days ago)

I would have thought that by microwaving, with some water in there as well would kill any contaminants. The microwave heats the water molecules and I know that I can get them really realy hot.

Then thing about PCing them is that they end up a little wet, even if i put them in foil. And then if I dry them with a paper towel, well that defeats the purpose of PCing them. No?


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OfflineAlsetAlokin
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Registered: 07/30/20
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Re: Microwave sterilization of Pertri dishes [Re: GermanShaman]
    #27135837 - 01/08/21 05:21 PM (3 years, 20 days ago)

Big foot nailed it with the search.

I'd recommend the oven.

You're still gonna have moisture in those plates if you plan on filling them with water in the microwave...


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