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Invisibleeatyualive
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EAT SWABS PLATES™ * 9
    #26341141 - 11/23/19 08:07 PM (4 years, 2 months ago)

:chief: EAT SWABS PLATES :chief:
A Step By Step Guide




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Hello Shroomery!  :hi:

:vibin:



This is how I inoculate plates with spores.

I used to use a spore loop where I would have to sterilize the loop everytime I would scrape spores onto a plate. With swabs I find it far simpler and I don’t have to sterilize anything right before swabbing the spores onto the plate. It’s quick, it’s easy and I can make enough swabs in one 30 minute pressure cook to last a year or two. They are also awesome because you can keep them wrapped prior to putting them in the SAB then open when you need them.

SUPPLIES FOR SWABBING

  • Sterile Swabs. Synthetic are preferred but cotton will work.





  •   Pasty Plates: make your pasty plates on the firmer side by adding a little more agar to the mix so when you swab you aren’t puncturing softer agar.



  • a dry clean OG SPORE PRINT™



  • 70% isopropyl alcohol


  • spray bottle with a fine mist


  • SAB






PROCEDURE

This entire process takes around 5 minutes if you are slow.

1) Setup your SAB

I usually use a wet SAB but use what procedures you are comfortable with. I flip my SAB upside down and use the lid as the work area. In addition, I use magnetic vent covers to let the air settle between clean work.

First I douse the entire inside, tops and bottom of the SAB with the iso in the spray bottle. I do not wipe with a towel.  I leave the iso heavily misted and dribbling on the sides, top and bottom so I work my SAB wet the entire time.

I close the vent covers and leave them closed. I only open the vent covers when I place items in the SAB or I am placing my arms in to do work.

2) I put on gloves, douse my hands and arms up to my elbows with iso.  I place all my supplies in the SAB. The pasty plate, foil covered swab, spore print, paper towel drenched with iso for sanitizing. I also re douse my gloves and forarms with iso anytime I take them out of the SAB before placing them back in.


3) The pasty plates are covered with foil so I Remove the foil, wipe the entire plate with the iso towel and loosen the lid. The lid is left on until you are ready to swab the plate.

The sterile swab is wrapped in foil so this is placed Directly next to the pasty plate and print. I keep everything in close proximity so the movements are not reaching far.  I use a steady work pace. Not too fast not too slow.

I unfold the side flaps of sterile swab foil but leave the longer side flap closed. I do not expose the swab yet. This is so when I am taking the swab, I can lift the foil easily to access the swab before I am exposing the swab to the SAB. You can see in the image below how the side pieces of foil are folded.



4) I close my vent covers prior to doing any work. I let the air settle for one to two minutes before I do the work. I then douse my gloves and forearms before opening the vent covers to do the work.

5) once the air is settled, take your print foil out of the plastic wrap. I unfold 3 sides of the foil but do not open the print yet until I am ready to make the swab swipe.

This is the way I wrap my prints. What it does is allow you to open the print easily. The top part of the foil can act as a shield from things falling from above when you use the swab to swipe the print. You can also flip the print upside down with the print facing the bottom of the SAB. My spore prints are wrapped nicely and in a plastic bag. In addition, the prints unfold very easy and are sealed nicely. It makes doing this type of work very simple. I feel the way you fold your prints is a key factor in getting your clean work streamlined and efficient.







5) With your working hand you unwrap your swab. With your other hand you will simultaneously open the print. I flip the print upside down so the face of the foil the print is on is facing the SAB floor.  I hold one side of the qtip with my gloved hand and use the untouched side to swipe spores. I make sure I twirl the tip as I swipe the print so I cover all sides of the swab with spores. It should look purple at the top like this.



6) I put the print down and fold the top back over the print.
I turn my pasty plate 45- 90 degrees to the SAB surface. The lid and side of the pasty plate act as a barrier to anything falling from above.
I use my thumb to slightly open the lid. While my fore finger holds the top of the plate open and my 3 other fingers hold the pasty plate. I swipe my qtip on the plate in a zig zag or streaking pattern. While I do this I am also slightly rotating the qtip to maximize the spore distribution. This is a very subtle and smooth motion. Nothing too hard so you do not pierce the agar surface. You should gently rub the swab on the agar and it will look unscathed on the surface after you are done. (Remember the note about a slightly firmer agar for swabbing spores).





7) put the lid on. Then label your plate. Done.

8) a few days later your plate will look like this. If you flawed your technique and any contamination is present you would then proceed to transfer to clean up the culture. There are a number of guides on the forum on how to clean up a culture.



Many people spend tons of time and transfers to get things growing for them. I at most do two transfers and 90% of the time the transfers look like this from multispore. My philosophy is this, you can spend months and months making a culture look perfect on a plate with transfer after transfer. Some people make a monoculture. Even then, until you fruit the culture you really don’t know what you are getting. Let’s say you spend 2-3 months making this culture look awesome on a plate. You spawn and fruit the culture and it performs like shit. You just wasted two months of your time on an IF. Something I could have found out after two transfers. I don’t discourage doing the cleanup. It’s fun, but I also don’t go over 2-3 transfers at most. Yes, sometimes you can’t get the culture clean until you do more transfers. I honestly never experience this issue. I can get full canopy flushes off multi spore and two transfers. So personally I don’t spend more time on the transfers as I do taking a good specimen clone off of a good flush. Narrow the genetics to what you like, repeat. 2 transfers should be an average with awesome performance. That’s my personal opinion so take it as you will.

This is a Texas yellow cap culture from two transfers below. The initial spores were swabbed to a pasty plate.



After you swab plates it is time to start preparing grain jars. First make the lids.

HOW EAT ROCKS HIS LIDS™


Edited by eatyualive (12/12/19 10:44 AM)


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InvisiblefahtsterM
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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #26356247 - 12/01/19 08:49 PM (4 years, 2 months ago)

This thread was moved from the user's journal.

Reason:
Eats request


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InvisiblePsicomb
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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #26356285 - 12/01/19 09:20 PM (4 years, 2 months ago)

Beautiful stuff Eat!!  Hell yeah :rockon:

This is a very clear, smooth sounding tek.  Swabs always confused me so it's nice seeing that using them can be both effective and easy.  Definitely will be giving this a go shortly.


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Edited by Psicomb (12/01/19 09:21 PM)


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InvisibleTormatoMFacebookDiscordReddit
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Re: EAT SWABS PLATES™ (moved) [Re: Psicomb]
    #26356344 - 12/01/19 09:54 PM (4 years, 2 months ago)

:rockon:


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: Tormato]
    #26356838 - 12/02/19 08:20 AM (4 years, 2 months ago)

Your welcome. It’s really easy. I still have a half pint full of the swabs I made a year ago. So they will last a while. I generally use them to start prints or swab gills for things like ape and PE. The part I like about swabs is that you don’t have to flame the loop and wait for it to cool. So when you get down to doing the work it’s quicker. Over time it’s definitely more efficient. I always found spore loops a little quirky but that’s just me.


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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #26356851 - 12/02/19 08:42 AM (4 years, 2 months ago)

Nice write up! I seen this in your link list for the past few days and have been wanting to read it.  I just swabbed some plates for the first time over the weekend.  Glad to see this so I could confirm that I did it properly.


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InvisibleSunnyDayze
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Re: EAT SWABS PLATES™ (moved) [Re: One of Us]
    #26356939 - 12/02/19 09:59 AM (4 years, 2 months ago)

Nice write up!  Awesome you took the time to make such detailed and illustrated instructions for all of us.  Thanks.


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OfflineSFS96
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Re: EAT SWABS PLATES™ (moved) [Re: SunnyDayze]
    #26357004 - 12/02/19 10:47 AM (4 years, 2 months ago)

Good stuff Eat:rockon: I going to make some swabs up today


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Re: EAT SWABS PLATES™ [Re: eatyualive]
    #26415536 - 01/04/20 05:47 AM (4 years, 1 month ago)

😎👍


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OfflineZakkery
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Re: EAT SWABS PLATES™ [Re: YogiBear]
    #26415545 - 01/04/20 06:04 AM (4 years, 1 month ago)

I like this. Just prepping my first round of mini rounds to start off my agar journey. I'll be using this Tek for sure.


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OfflineInthepit
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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive] * 1
    #26429160 - 01/12/20 05:46 AM (4 years, 1 month ago)

Awesome write up!
Darn, I was hoping someone else would ask this question!
So, one jar of swabs is lasting a long time. Does that mean you swab more than one plate with the same swab?
Also, did you see CBK said he doesn't use foil? He PC's a jar of swabs and takes straight from there.
I dig your :chief: OG SPORE PRINT TEK™:chief:  :thumbup:


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: Inthepit] * 1
    #26429405 - 01/12/20 09:59 AM (4 years, 1 month ago)

Yes you can swab multiple plates with the same swab. I usually do two or depending on what I have available. I did see cbk say that he doesn’t wrap them until after he swabs it. You could do that. I like having the foil already ready to put the swab back in after I initially wrap it. I feel like I’m exposing the swab to the sab less. I think either would work. I just don’t like doing the extra movement of the fold after while the swab is exposed. So I’ve got my foil pre folded and it’s quick to close the folded foil and seal for me. Personally, I think if your technique is good following steady movement, and other principles like not moving your bare skin over things, you will have success.

There are definite ways you don’t wanna do something but there are also 10 ways to skin a cat. If it accomplishes your overall goal and is easier for your method, I always say try everything you can. I used to do it the same way as well.

Thanks and same principles for taking prints work from an outside direction so your not putting your bare skin over a print and stuff like that should be assumed for the layman. I work on the outside when I take prints. There are also tweezers you can flame sterilize while moving a cap to place over the foil without exposing your skin over anything. When wrapping the prints up I work from right to left in a row then go to the next row so each print is folded and no print is lifted above another printX in addition the way I fold the sterile side underneath the print, that side is not exposed until you fold it over the print and seal it. So it’s very minimal.


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OfflineInthepit
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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #26429446 - 01/12/20 10:24 AM (4 years, 1 month ago)

Oh cool! And thank you for so much reply.
Quote:

There are also tweezers you can flame sterilize while moving a cap to place over the foil without exposing your skin over anything.



Oh awesome, hadn't considered that, I have a 9" tweezer, not home so can't picture it, but ya, another awesome tip! Thank you for all you do!

Oh! And I'm so inspired by the card you include sending out prints! Ha! I haven't even had a fruiting yet, but I'm already trying to come up with a cool card for prints!


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: Inthepit]
    #26429854 - 01/12/20 02:54 PM (4 years, 1 month ago)

Yeah 9 inch tweezers. We used to hold roaches with them.



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Edited by eatyualive (01/12/20 03:00 PM)


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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #26429878 - 01/12/20 03:12 PM (4 years, 1 month ago)

Those tweezers are awesome.
Mine also came with giant forceps in a cool little pouch.

They’re so thick I gave up on sterilizing them though, all iso.


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Edited by A.k.a (01/12/20 03:13 PM)


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: A.k.a]
    #26429915 - 01/12/20 03:35 PM (4 years, 1 month ago)

Yeah just sanitize if your using it for caps.


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InvisibleRogerRabitsMycoBra
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Re: EAT SWABS PLATES™ (moved) [Re: fahtster]
    #27126005 - 01/04/21 11:57 AM (3 years, 1 month ago)

Hey great tek man thank you . Have you ever done it in front of a flow hood? I know you said do whats most comfortable for you but I wasn't sure if you meant whatever SAB tek is most comfortable to you..

Also , I have a sort of unrelated question.How many sterile  swabs can you use per dish ?Before this post I would have assumed 1 swab to 1 dish to keep everything sterile and individualized, but Ive seen people say they use the same swab on 4 dishes. Have you ever tried this?


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: RogerRabitsMycoBra]
    #27135643 - 01/08/21 04:09 PM (3 years, 1 month ago)

I have not because i do not have one. But it should work.

I usually use the swab on one plate then discard. Thats me though.


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Re: EAT SWABS PLATES™ (moved) [Re: eatyualive]
    #27169179 - 01/25/21 01:43 PM (3 years, 22 days ago)

Just curious what the note is for firmer agar when using swabs. Did I miss that?

I'm about to use a swab for the first time and want to make sure I do it right. The plates I have on hand are 9g agar, 8g DME, 250ml water. Does this seem like it will work well?

I need to pour some more plates anyway so if you think a different recipe would work better I'll give it a try. I just want to make sure I get something to germinate since these are my only two swabs.

Thanks for the writeup!


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Invisibleeatyualive
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Re: EAT SWABS PLATES™ (moved) [Re: bigfootscreepyuncl]
    #27178016 - 01/30/21 01:28 PM (3 years, 17 days ago)

Softer for germinating spores.


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