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OfflineAnother_Hobby
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Registered: 09/23/20
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Agar Transfer Again?
    #27133083 - 01/07/21 02:14 PM (3 years, 21 days ago)

All five of these were from a Golden Teacher spore syringe in November and then transferred on 12/5 and then again on 12/15. The growth isn't that great/even yet as compared to what I am seeing online (BOD's Comprehensive Agar TEK). I'm using 10g agar, 10g DME, with 500g tap water for my dishes. Temperatures are probably 68 during the day and 60 during the night (drafty house).

Should I transfer all five again or pick the best among these? I can't figure out which is best other than the one that looks the strongest and is furthest from the center. 



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OfflineHaywire
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Registered: 12/29/13
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Re: Agar Transfer Again? [Re: Another_Hobby]
    #27133118 - 01/07/21 02:32 PM (3 years, 21 days ago)

that top right one and bottom left one look ok.
Must say, it looks a bit strange.
I don't like to isolate too much when germinating, I just go one or two transfers after germination and then go to grains.
I prefer to let the substrate narrow down the genetics.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineMarxcelium
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Re: Agar Transfer Again? [Re: Haywire]
    #27133165 - 01/07/21 02:57 PM (3 years, 21 days ago)

I was gonna say, these all look off to me. If they were in my lab, I'd consider them all contamed and wouldn't go to grain with them, but I don't know if the myc is reacting in a funky way to you agar mixture or what. I've ready that myc can grow into certain agar mixtures rather than across, but I've never seen it in my own work.

Top right does look best of the bunch, I agree, though.


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OfflineShroomoisseur
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Re: Agar Transfer Again? [Re: Marxcelium]
    #27133320 - 01/07/21 04:04 PM (3 years, 21 days ago)

Get clean plates, don’t worry about it looking even or pretty. Just get clean cultures and take them to grain; fruit it and then find fruits you want to clone. What’s the point of trying to keep isolating if you don’t know how it’s going to produce?


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OfflineTigerStrike
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Re: Agar Transfer Again? [Re: Shroomoisseur]
    #27133332 - 01/07/21 04:07 PM (3 years, 21 days ago)

What to do strongly depends on your goals. If you want a bunch of isolates to get heaviset fruiting possible yes transfer all and isolate all and have deep pockets then fruit test ect.

If you want to find just vigourious mycellium then choose the 2 or 3 best.

If you want to learn to juggle just keep 3 and don;t make any transfers and get a juggling instruction book.

What are your goals and I may be able to help more! :laugh:


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OfflineAnother_Hobby
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Re: Agar Transfer Again? [Re: TigerStrike]
    #27133606 - 01/07/21 06:16 PM (3 years, 21 days ago)

The ultimate goal is to get to monoculture; because that tests my ability to make agar, pour agar in a SAB, inoculate the first plates from a spore syringe, identifying sectors on a plate, and transferring single sectors to produce a monoculture.

I can keep BRF cakes going to produce enough shrooms for myself, agar is just for fun. I am wondering if my temperatures are causing slow growth and when I transfer I am taking too big of pieces and getting more strains that I should.

Here is a better pic with the light shining from the side, my first pic was crap.


Edited by Another_Hobby (01/07/21 06:53 PM)


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OfflineHaywire
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Re: Agar Transfer Again? [Re: Another_Hobby]
    #27134346 - 01/08/21 12:55 AM (3 years, 20 days ago)

If you inoculated in november and are only two transfers in, your mycelium is very slow.
usually I can do a transfer every week.

but this being said, I still stick to my point to use the mentioned plates on grains and grow it out. then select a couple of good fruits/clusters to clone.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineProfessor X
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Re: Agar Transfer Again? [Re: Haywire]
    #27134349 - 01/08/21 12:58 AM (3 years, 20 days ago)

I did some plates early December that are pinning right now... I've got some real monsters on my ks plates, like .1 dry


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


Edited by Professor X (01/08/21 12:59 AM)


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OfflineBrian Jones
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Re: Agar Transfer Again? [Re: Another_Hobby]
    #27134437 - 01/08/21 03:29 AM (3 years, 20 days ago)

Quote:

Another_Hobby said:
The ultimate goal is to get to monoculture; because that tests my ability to make agar, pour agar in a SAB, inoculate the first plates from a spore syringe, identifying sectors on a plate, and transferring single sectors to produce a monoculture.

I can keep BRF cakes going to produce enough shrooms for myself, agar is just for fun. I am wondering if my temperatures are causing slow growth and when I transfer I am taking too big of pieces and getting more strains that I should.

Here is a better pic with the light shining from the side, my first pic was crap.





Keep in mind that when your trying to create an isolate it's time consuming and you don't know what you're isolating till it's done.

I always found going MS to agar to be a huge pain. For me it makes more sense to start by shooting up PF tek jars (which usually work fine from syringes). I don't bother with a fruiting chamber, just fruit them in a jars (see Bodhi's beginner's tek). Select a nice looking shroom and clone them to agar. Then you can go grain. I still don't bother to try to isolate, but every time I'm using petris to inoculate grain jars, I start several more petris. But if you do want to isolate you're starting with a leg up because you cloned a good looker.


--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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OfflineEbilPhish
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Re: Agar Transfer Again? [Re: Brian Jones]
    #27134489 - 01/08/21 05:10 AM (3 years, 20 days ago)

Growing then cloning makes more sense to me. Just transfer enough to make sure there's no contamination.

Just because the mycelium is fastest on a petri dish doesn't mean it's the best for fruiting. Also you will have to transfer a load probably still end up with a heap of cultures so your still going to have to grow and clone anyway.

Petris have different nutriants/substrate and no contams to contend with. On the other hand growing under regular conditions means you get something that managed to take over enough of your substrate to fruit.


Edited by EbilPhish (01/08/21 05:11 AM)


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OfflineAnother_Hobby
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Re: Agar Transfer Again? [Re: Haywire]
    #27135498 - 01/08/21 03:14 PM (3 years, 20 days ago)

I'll probably grab a clone from one of my BRF cakes and play around with that. No matter what I do, it is all stuff I can learn from and see what works for me. Thanks for the tips.


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OfflineAnother_Hobby
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Re: Agar Transfer Again? [Re: Brian Jones]
    #27135510 - 01/08/21 03:17 PM (3 years, 20 days ago)

I can see why going from a syringe to agar is a pain, but it's a great learning experience. I'll have to do a clone from the next big shroom that comes up on my BRF cakes. Largest one so far was 1.54g dry, so that was cool! Dabbling in all of the methods is the most fun and rewarding.


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OfflineAlsetAlokin
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Registered: 07/30/20
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Re: Agar Transfer Again? [Re: Another_Hobby]
    #27135645 - 01/08/21 04:09 PM (3 years, 20 days ago)

I'd transfer top right at 12 o clock and 2 o clock.



If you're coming from MS it's best to pick the best looking plate and then only take transfers from that. Saves on plates.


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OfflineInthepit
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Re: Agar Transfer Again? [Re: Brian Jones]
    #27136897 - 01/09/21 06:33 AM (3 years, 19 days ago)

Quote:

Brian Jones said:
I always found going MS to agar to be a huge pain. For me it makes more sense to start by shooting up PF tek jars (which usually work fine from syringes). I don't bother with a fruiting chamber, just fruit them in a jars (see Bodhi's beginner's tek). Select a nice looking shroom and clone them to agar. Then you can go grain. I still don't bother to try to isolate, but every time I'm using petris to inoculate grain jars, I start several more petris.



This is really interesting. I haven't had any luck with syringes and am getting really gun shy.
I'll keep at in LAGM21 because it has to go to agar first. But really, can it be true
that going directly from syringe to PF TEK jars won't be a contam fest? I skipped that TEK and
started with agar, so that's why I'm ignorant. :laugh2:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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OfflineTigerStrike
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Re: Agar Transfer Again? [Re: Inthepit]
    #27137991 - 01/09/21 04:07 PM (3 years, 19 days ago)

Pf tek has a low contam rate even when there ARE contamination. That is the beauty of tf tek and why I am so sad seeing it get shit on especially moving cakes to sgfc its noobish and most turn their nose up wether thet realize or not as noobs can detect these feelings toward the TEK THAT LAID THE GROUNDWORK for all these complicated teks


push monos. I would say a few years ago the most important thing is PF tek adleast performing it a few times just to ecperience our history, now there are so many high budget videos teaching you how momos are made. Now I really dunno anymore whats important for a noob to learn.
\
Not only that they can be sterilized without a PC

So yeah all you need is verm jar rice to grind or rice flour pot stove spores water

like 11dollars and 34 cents worth of shit


Edited by TigerStrike (01/09/21 04:10 PM)


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OfflineProfessor X
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Re: Agar Transfer Again? [Re: AlsetAlokin]
    #27138032 - 01/09/21 04:35 PM (3 years, 19 days ago)

Quote:

AlsetAlokin said:
I'd transfer top right at 12 o clock and 2 o clock.



If you're coming from MS it's best to pick the best looking plate and then only take transfers from that. Saves on plates.


I second that


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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OfflineInthepit
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Re: Agar Transfer Again? [Re: TigerStrike]
    #27138179 - 01/09/21 06:16 PM (3 years, 19 days ago)

Thank you TigerStrike, I was hoping you would respond!


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