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OfflineModecom
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Rye jar health check and some questions
    #27132804 - 01/07/21 12:07 PM (3 years, 21 days ago)

Hello friends. So about 3months ago I got into all of this and with the help of many of you, and some time digging in I seemed to got the hang of it and successfully did everything on the first go. Got 5flushes off of 2shoe boxes, and my mono is doing great as well.


Round 2. Is where I'm starting to get some hesitations on what's going on.
Some info:

All jars have been colonizing for roughly a month now. Jars are 700ml(so 0.7quart?:laugh:). I used a pretty small wedge of a agar, Like around or smaller than a rye grain. The agar plates were made around 3months ago when my first jars were started. I used 3 different plates to make 12jars. Some of them are not yet 100%, but super close. Most of them are "done" and this is where I'm a bit skeptical. My first jars I did, all turned out "pretty white" comparing to how "whispy" these look. The grains I used this time around were some Eco rye grain I picked from the store, and well they deffo look more light in color compared to the ones I used the first time(Not sure if this can add to the higher whispiness look). THe very blu on some jars is just paint:D,dont ask:D.


I'll be posting some photos now and they are off different jars/agars, but all of them kind of looked very simillar when ~100% done. The ones that are done for around a week now(I let them sit longer to observe), got whitter, and dont show nearly as much wispiness and see through clear grains.


This is a jar that was basically ~100% had that whispier clear light grain look, This is like ~48hrs after shake. It still seems to have that whispy barely barely seeable mycelium on the grains, but also more clear white white myc spots.




So is this just tormentose mycelium? Or bacteria problems? My agar plates were sitting in room tep kind of, So even if they had some rhyzo sectors I tried taking, it also had fluffy myc(Areal myc?) kind of everywhere I imagine. Anywho Ideas are greatly appreciated :laugh:


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OfflineTheMagicConch
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Re: Rye jar health check and some questions [Re: Modecom]
    #27132820 - 01/07/21 12:20 PM (3 years, 21 days ago)

Doesn't look bacterial to me, could possibly be cobweb mold? Could also be tomentose mycelium, it can be really hard to tell.

See here


--------------------
"You're not losing your mind, your mind is losing you"

Good info and Teks:
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Agar Portal-24hr soak WBS-Coir Prep-Shoebox Tek-Psilocybe (Agaricales) Life Cycle Discussion Thread


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OfflineDr.Sparkle
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Re: Rye jar health check and some questions [Re: TheMagicConch]
    #27132828 - 01/07/21 12:22 PM (3 years, 21 days ago)

A month for a jar to colonize is a long time. I aim for 10-12days before they are fully colonized and ready to go. Sometimes they sit a little longer.

It takes around 48hrs to really get going again, if they are good they should look completely different in the next 12hrs. Sometimes I have jars that do this and I still use them, unless they were to show any other signs of contamination. Once they get tipped into a bag, they spring to life I figure sometimes the gas exchange wasn’t great for that particular jar. Obviously if it shows other signs of contamination it would get tossed.



Edited by Dr.Sparkle (01/07/21 12:28 PM)


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OfflineModecom
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Re: Rye jar health check and some questions [Re: Dr.Sparkle]
    #27132858 - 01/07/21 12:38 PM (3 years, 21 days ago)





Quote:

Dr.Sparkle said:
A month for a jar to colonize is a long time. I aim for 10-12days before they are fully colonized and ready to go. Sometimes they sit a little longer.

It takes around 48hrs to really get going again, if they are good they should look completely different in the next 12hrs. Sometimes I have jars that do this and I still use them, unless they were to show any other signs of contamination. Once they get tipped into a bag, they spring to life I figure sometimes the gas exchange wasn’t great for that particular jar. Obviously if it shows other signs of contamination it would get tossed.






I can't even begin to imagine how it could be done in 2weeks :laugh:. Maybe with a isolate... I suppose as far as my inoculation goes, It is far from a isolate. I also as I said used a pretty small piece of agar. I have a friend tho threw in one ~1.5x the size of a bottle cap, got his jars almost done in 2weeks. As I said some of my jars were 100% after 3weeks. Those few day differences I just accumulated to me using a pretty small piece.  Ah also My temp is usually around 73-75f, so maybe that slows me down a tad tad bit. Thanks for your input though! Its reassuring to know some people see something similar :laugh:


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OfflineCrackatoa
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Re: Rye jar health check and some questions [Re: Dr.Sparkle]
    #27132870 - 01/07/21 12:43 PM (3 years, 21 days ago)

Like Dr said, give them another day to see how they look. 48 hours is still in that window where they will still look weird from the shake. Cobweb mold usually goes very quickly and they way I can tell it's how wispy it is and it goes so quickly you know it can't be mycelium. A month isn't that bad when you're inoculating a jar with a piece of agar the size of a grain. If you cut up a 100 mm petri and threw in a jar and it took a month I would see a bigger issue. I would give it a couple more days and if it still looks the same I would assume it's a bacterial issue.


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OfflineDr.Sparkle
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Re: Rye jar health check and some questions [Re: Modecom]
    #27132883 - 01/07/21 12:51 PM (3 years, 21 days ago)

Exactly what cracktoa said.

Your temps are great! The quicker the mycelium colonizes the grain the less time contamination has to get a foot hold. Its always a race between contams and mycelium.

Your agar is very old at 3months! Next time if you are not going to use that plate wrap it like mad in cling film, place it into two zip lock bags. Make sure it does not freeze. When you are ready to use it, bring it out let it warm up and cut a small piece of myc from the leading edge and transfer it to a new dish. Once the new dish growth is 1cm away from the edge use that for your jars. I would not recommend to use a 3month old plate that has sat at room temp to inoculate grain.


Edited by Dr.Sparkle (01/07/21 12:51 PM)


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OfflineModecom
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Re: Rye jar health check and some questions [Re: Crackatoa]
    #27132892 - 01/07/21 12:56 PM (3 years, 21 days ago)

Quote:

Crackatoa said:
Like Dr said, give them another day to see how they look. 48 hours is still in that window where they will still look weird from the shake. Cobweb mold usually goes very quickly and they way I can tell it's how wispy it is and it goes so quickly you know it can't be mycelium. A month isn't that bad when you're inoculating a jar with a piece of agar the size of a grain. If you cut up a 100 mm petri and threw in a jar and it took a month I would see a bigger issue. I would give it a couple more days and if it still looks the same I would assume it's a bacterial issue.



Could them being drier also promote tomentose growth? I definitely made these drier than my first batch. Because in my first batch I had like 1-2-3 wet looking grains per jar that the myc seemed to not want too touch at all(I was like all over the idea that they were bacteria...). But 2 months later after spawning them I'm on my 5th flush from those and happier than could be :laugh:. So once again I'm in this "trippy" mindset that smth isnt quite right. But I'm a person of overthinkiness so forgive me :laugh:.

Quote:

Dr.Sparkle said:
Exactly what cracktoa said.

Your temps are great! The quicker the mycelium colonizes the grain the less time contamination has to get a foot hold. Its always a race between contams and mycelium.

Your agar is very old at 3months! Next time if you are not going to use that plate wrap it like mad in cling film, place it into two zip lock bags. Make sure it does not freeze. When you are ready to use it, bring it out let it warm up and cut a small piece of myc from the leading edge and transfer it to a new dish. Once the new dish growth is 1cm away from the edge use that for your jars. I would not recommend to use a 3month old plate that has sat at room temp to inoculate grain.




Yeah I'm aware. I was just thinking I'd get some clones off my first batches, but than life turned a weird way and I ended up being well "lazy" and not in the mindset to do anything. I did take some spore prints and the very least! :laugh:. Bet I see how older one can be a bad factor one. I'll deffo make some new plates in the days to come, and use those prints to start fresh ones to keep better care off. Thanks!


Edited by Modecom (01/07/21 01:00 PM)


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InvisibleJosex
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Re: Rye jar health check and some questions [Re: Dr.Sparkle]
    #27132899 - 01/07/21 12:59 PM (3 years, 21 days ago)

Imo those jars are trouble. That kind of wispy is always suspect and among other things it can mean you didn't cook/hydrate the grains well enough. I wouldn't be surprised if in the second set of pics you had some kinda mold.


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OfflineModecom
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Re: Rye jar health check and some questions [Re: Josex] * 1
    #27132913 - 01/07/21 01:04 PM (3 years, 21 days ago)

Quote:

Josex said:
Imo those jars are trouble. That kind of wispy is always suspect and among other things it can mean you didn't cook/hydrate the grains well enough. I wouldn't be surprised if in the second set of pics you had some kinda mold.




I can deffo see them being too dry. But wouldn't a mold get easily nuked during the PS cycle? Which I deffo did right. Hydration part thou.. First time thinking I made em too wet, now I deffo think I made em too dry. 3rd timez gonna be the charm:D


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InvisibleJosex
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Re: Rye jar health check and some questions [Re: Modecom]
    #27132923 - 01/07/21 01:12 PM (3 years, 21 days ago)

You're good, this is all a learning experience, you have to fuck up to learn.

I'm not sure you have mold there but it very well may be. I know I've had that type of sneaky mold before. Mold or not, those jars look toast. I would toss them but if you don't want to and prefer to spawn them just keep a close eye on them and toss at the first sign of fuckery.

Yes the PC destroys molds easily, but it could have been introduced via technique or maybe you had some mold meshed in with the culture. Again, not sure you have mold.


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OfflineModecom
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Re: Rye jar health check and some questions [Re: Josex]
    #27132932 - 01/07/21 01:17 PM (3 years, 21 days ago)

Quote:

Josex said:
You're good, this is all a learning experience, you have to fuck up to learn.

I'm not sure you have mold there but it very well may be. I know I've had that type of sneaky mold before. Mold or not, those jars look toast. I would toss them but if you don't want to and prefer to spawn them just keep a close eye on them and toss at the first sign of fuckery.

Yes the PC destroys molds easily, but it could have been introduced via technique or maybe you had some mold meshed in with the culture. Again, not sure you have mold.



Fair enough. I'll deffo play around with at least some of the "healthier" looking ones. And I'll try too update what the future holds.


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