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Offlinesinubrius
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Registered: 06/02/18
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Can someone quickly double check my plates?
    #27132320 - 01/07/21 07:16 AM (3 years, 1 month ago)

Got some transfers here, a few small questions about each of them



This plate (1) is really fluffy and has some weird ring-like formations when viewed under a light from underneath. The edges look kinda rhizomorphic in these pictures, but I'm generally unsure. It's quite raised from the surface of the plate. Is this common/okay, or is it contam?




This plate (2) I'm kinda confident with, actually just putting it here to check.



This plate (3) I'm unsold on. If you look closely at the view from underneath, near the centre there's this weird circular stain, where the agar seems to be a different color. I'm worried that this is some kind of dormant/piggybacking bacterial contam and I'm wondering if anyone else has any experience?

All three of these plates eventually lead back to the same set of plates if you go back far enough, and each one has some sort of suspicious (as far as this noob is concerned) fuzz sitting ontop of what otherwise looks like somewhat rhizomorphic mycelium.

Is it possible to get other molds tangled up into the mycelium while it is growing? If so/if this is what I've got, what can I do to kill it off?

[Blue agar is MEA - 10g/10g/500ml]
[Brown agar is GWA - 10g/250mlGW/250mlWater]
Using an adapted version of Pasty's no-pour that has worked fine in the past.

I tried my best to get as clear cut photos as I could!!

Your help is greatly appreciated :smile:

**Edit** lmk if the photos are too shit and I'll try and find a better camera.


Edited by sinubrius (01/07/21 07:24 AM)


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OfflineMarxcelium
Mushroom Instrumentality Project

Registered: 05/12/20
Posts: 127
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Last seen: 2 years, 2 months
Re: Can someone quickly double check my plates? [Re: sinubrius]
    #27132615 - 01/07/21 10:33 AM (3 years, 1 month ago)

The only thing I feel confident telling you here it that myc will digest the food coloring in the agar, so you probably don't need to worry about the discoloration in the center of the culture.  That's totally normal.

I can't really tell 100% whether you've got ride along mold, which, yes, is absolutely possible. Look for disorganized or powdery areas at the leading edges of the myc. It's one of the unfortunate drawbacks of no-pours that you sometimes end up with containers that make visibility a challenge, particularly in photos.

I'm sure someone with more confidence than me will chime in.


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Offlineclockworkshroom
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Re: Can someone quickly double check my plates? [Re: Marxcelium]
    #27132657 - 01/07/21 10:46 AM (3 years, 1 month ago)

I'm new to mycology but have inoculated about 100 plates in the last month and looked at 1000s on here and other places online.

Obviously wait for someone who knows but they all look clean to me and not contaminated. They do look like further transfers could be beneficial, the fluff is most likely just tomentose mycelium growth on top of the rhizomorphic growth.

The transferred wedges look quite big which may be why there appears to still be some lack of uniformity after multiple transfers.


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OfflineAlsetAlokin
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Registered: 07/30/20
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Re: Can someone quickly double check my plates? [Re: Marxcelium]
    #27132672 - 01/07/21 10:52 AM (3 years, 1 month ago)

Rings are normal if they align with temperature fluctuations.

Take more transfers if you're worried about some piggybacking contam.

Take smaller transfers. Size of a grain of rice is ideal for me.


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Offlinesinubrius
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Re: Can someone quickly double check my plates? [Re: AlsetAlokin]
    #27133659 - 01/07/21 06:36 PM (3 years, 1 month ago)

Hi guys!!
Big thanks to each of you for these responses!!

Quote:

Marxcelium said:
I can't really tell 100% whether you've got ride along mold, which, yes, is absolutely possible. Look for disorganized or powdery areas at the leading edges of the myc. It's one of the unfortunate drawbacks of no-pours that you sometimes end up with containers that make visibility a challenge, particularly in photos.



So there are no powdery edges, or anything really beyond the leading edge of very straight and organized hairs, which I'm personally confident is somewhat rhizo. The disorganization that I'm seeing is kinda more ontop, which leads me to believe it's just some tomentose stuff ontop. Fluffier hairs sitting ontop of the culture that don't look too dissimilar from the leading edge, but they are fluffy and reaching into the air. Particularly in Plate (1), from the side profile it rises up, however even at the risen parts of the leading edge, hairs are organized and moving away in search of food.

Quote:

clockworkshroom said:
I'm new to mycology but have inoculated about 100 plates in the last month and looked at 1000s on here and other places online.

The transferred wedges look quite big which may be why there appears to still be some lack of uniformity after multiple transfers.



Quote:

AlsetAlokin said:
Rings are normal if they align with temperature fluctuations.

Take more transfers if you're worried about some piggybacking contam.

Take smaller transfers. Size of a grain of rice is ideal for me.



This is really useful! I'll take most of the plates I have and start making smaller transfers on them from now on so I can get some more uniformity in the structure. These plates have definitely been subject to wild temp swings that are currently outside of my control, so I'm glad to see the rings aren't mold, I almost ditched that plate lol.

One Last Question

Do ya reckon I'll be okay to make an LC out of one of these? Have made successful LCs from plates before, just wondering if its better to get something more isolated than these are. I'll still be further isolating anyway, just wanna get an LC on the boil while I'm doing that.

You have my infinite gratitude!!

**Edited for grammar/syntax


Edited by sinubrius (01/07/21 06:40 PM)


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OfflineAlsetAlokin
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Registered: 07/30/20
Posts: 182
Last seen: 3 years, 1 month
Re: Can someone quickly double check my plates? [Re: sinubrius]
    #27133688 - 01/07/21 06:44 PM (3 years, 1 month ago)

Questionable plates make questionable LC.

It can be frustrating, but way less than a dozen moldy jars.

If you want piece of mind, make (at least one) more transfer(s).


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InvisiblezMan
zMan


Registered: 09/08/12
Posts: 2,191
Re: Can someone quickly double check my plates? [Re: AlsetAlokin]
    #27133774 - 01/07/21 07:04 PM (3 years, 1 month ago)

If you make an LC make it out of plate 3.

They do look ok but like others stated unless you're in a rush (and in this hobby you shouldn't be)  make much smaller transfers. A good amount of time i sweat to myself I didn't even transfer anything (sometimes running the scalpel through mycelium after it was cooled down and then slicing the middle of my transfer plate gives me growth).


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Offlinesinubrius
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Re: Can someone quickly double check my plates? [Re: zMan]
    #27134183 - 01/07/21 10:25 PM (3 years, 1 month ago)

Patience is what got me there last time, so I'll temper myself and keep making transfers.

Thanks again for the advice, guys! :smile:


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