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Offlinepsy25
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Registered: 12/17/20
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Help with Liquid inoculation
    #27132197 - 01/07/21 05:07 AM (3 years, 21 days ago)

First, thanks for reading !

Second, if anyones got good TEK for liquid inoculation (using spore syringe to colonize spawn jars that have already been preped and PC'ed). that would be great.

Third,

So i had some problem with L.I this my first attempt

- Problems-

- its been 20 days and no sign of colonization ? (I used PC popcorn) I used around 1- 6 cl of liquid. Some jars had less, some had more.

- Some of my jars cracked at the bottom. Nothing heat related, I waited 24h for cooling. I dont have any gas exchange. Maybe that's the mistake? Can I use microspore tape as a good filter for gas exchange? if not what can i use from a home dep store instead?

- How much liquid should one use to inoculate ?

Thanks for reading and hopefully replying !

Lots of love,
psy


Edited by psy25 (01/07/21 07:16 AM)


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OfflineHappinessStan
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Re: Help with Liquid inoculation [Re: psy25]
    #27132276 - 01/07/21 06:34 AM (3 years, 21 days ago)

https://www.shroomery.org/forums/showflat.php/Number/22833314/fpart/1/vc/1
There you go, I'm literally about to try it myself, just finished pc'ing my water. :thumbup:


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Offlinepsy25
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Re: Help with Liquid inoculation [Re: HappinessStan]
    #27132313 - 01/07/21 07:15 AM (3 years, 21 days ago)

Ah nice ! thanks for the TEK !

I think I miss communicated a lil bit, I used spore syringes to begin with, I bought them. Im not trying to make spore liquid just yet hehehe


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OfflineHappinessStan
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Re: Help with Liquid inoculation [Re: psy25]
    #27132428 - 01/07/21 08:37 AM (3 years, 21 days ago)

Ah, ms syringe straight to grains is a bad idea. You really wanna try agar, it's cheaper and easier than people would think, and will leave you far more satisfied.
As for your jars ,they might be taking their time to germinate,they might be bunk. They should at least germinate without gas exchange.Maybe bacteria won before they could germinate?
For gas exchange, polyfill will work, rip up a teddy or cheapo cushion. Fold up and pull it through the hole till it's tight, snip/burn off any loose fibers.


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Offlinepsy25
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Re: Help with Liquid inoculation [Re: HappinessStan]
    #27134472 - 01/08/21 04:48 AM (3 years, 20 days ago)

Sweet sweet !

Thank you for your reply ! I will check out the agar ! Hopefully i can use the MS to make the agar !

hmm yeah maybe bacteria won, dont see how though, i got self healing injection ports and the lid never opened.

Strange how some of them cracked ! ill post pictures soon of that :smile: wonder if its happened to anyone else..

Is it essential for gas exchange? and do you need a SAB for using agar ?

Thanks for your reply happinessstan ! :laugh:


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