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OfflineUzimyco
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Anyone have link or pics of early stage agar inoculation?
    #27129824 - 01/06/21 07:24 AM (3 years, 1 month ago)

Hi All

I finally got my agar going and of course there are countless pics of well grown and colonized agar available both on this site as well as the internet in general.

I have been unable to locate any really early development pics for example, illustrating what onset type germination looks like.

My dishes show a varied degree of activity and started to with 1-3 days of spore inoculation.  Due to some less than perfectly exacting innoc. techniques (smears here, better zig zag there) the dishes exhibit fairly different looking characteristics.  Since this is really a first time agar run it is difficult to discern if I’m seeing things positive or negative.  Again if it was clearly a thick grown out plate of myc then yes it would be more obvious. I’m watching daily as I’m trying to learn any patterns or behaviors however without a frame of reference at this point, I couldn’t say if I’m growing mycelium or if I’m growing influenza :crazy:

Any such info or links would be most appreciated.

Thank you in advance!!


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InvisibleJosex
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27129830 - 01/06/21 07:30 AM (3 years, 1 month ago)

https://www.shroomery.org/forums/showflat.php/Number/25662166#25662166

This might be the thread your're looking for.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131690 - 01/06/21 08:54 PM (3 years, 1 month ago)

I appreciate the link but so many of those look like transfers to
Me? Not necessarily early onset spore growth?

Maybe I should post some pics and can get some advice. I’m not
loving what it looks like though currently.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131716 - 01/06/21 09:04 PM (3 years, 1 month ago)

Here are some pics. I swear I don’t know what the hell I’m looking at here.




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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131729 - 01/06/21 09:09 PM (3 years, 1 month ago)

I'm afraid there's only bacteria on all plates. But in the third pic there seems to be a little patch of mycelium that looks like it could grow into something you could take a clean transfer from. What was your process? What did you use to inoculate your plates?


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Offlinestarbones
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131734 - 01/06/21 09:11 PM (3 years, 1 month ago)

Plates 1 to 5 are contaminated with bacteria.
I imagine bacteria will begin to show on plates 6 and 7, unless it has and my eyes are dogshit right now.

That bacteria follows your streaking so somethings up with your technique or worse here.


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InvisibleDoctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: starbones] * 1
    #27131748 - 01/06/21 09:17 PM (3 years, 1 month ago)



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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27131769 - 01/06/21 09:27 PM (3 years, 1 month ago)

Don’t know if it’s against the rules?

Any experts willing to give some limited direct instruction?

That means I pay for say 15 mins on the phone... vid chat etc?  My platform allows for me to give you a site address and a code. No download on your side. Just hit the site - enter the code - were talking.

Cant fathom how it’s Possible. Zip you over $50-$100 for 15 mins. Clear my issues once and for all.

Any taker ? Or is that a gargantuan no-no?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131781 - 01/06/21 09:35 PM (3 years, 1 month ago)

:lol: That's a new one. Never seen nobody asking to talk on the phone to someone to fix their agar issues.

Man we do this everyday here on the forum, helping people overcome their issues. I think nobody is going to want to call you. :cop:

Tell us your process and I'm sure we can lend you a hand.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131786 - 01/06/21 09:39 PM (3 years, 1 month ago)

Streaks were done using sterile disposable innoc loops individually packaged. In SAB.

Agar PC at 15 psi for 30+ mins.  Poured allowed to cool in SAB.

ISO cleaned - uvc irradiated. Enough ozone to kill a horse. Spores protected from uvc naturally. Don’t know how anything could live through it?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131793 - 01/06/21 09:40 PM (3 years, 1 month ago)

You used a print?


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InvisibleDoctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131797 - 01/06/21 09:42 PM (3 years, 1 month ago)

Damn, we should start a mush cult call center. I'd do it for free but my house is shut down for the night. Everyones asleep except for me cause I'd rather hang out with you guys and hate myself when I have to get up for work in the morning.

Spores can take as long as 3 weeks to germinate. When they do, it'll look like little white hairy spots. In that link that I shared, you'll see a blue plate with a Z pattern that has little white areas all around the spore clumps. Those are what you're looking for.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131799 - 01/06/21 09:43 PM (3 years, 1 month ago)

Well I agree. Sounds a bit weird. But I don’t see an issue with the transfer of info. Vids all over you tube and like I said. Could go web based with no client. I know the technology. I sell it for a living.

Just figure I don’t expect anyone’s time and experience for free. Don’t mind paying for advice personally but I get it sounds weird. Like I said. Just frustrated with running into trouble over snd over


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131807 - 01/06/21 09:46 PM (3 years, 1 month ago)

Yes green from b+ print. Blue from GT print. But I want suggesting right now anyway. At a time that was convenient for someone.

I work with consultants in business everyday. It’s normal for me although not the same subject matter but that was my thought process.


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InvisibleDoctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131809 - 01/06/21 09:47 PM (3 years, 1 month ago)

That looks like myc. Wouldn't hurt to watch it and transfer before the bacteria gets to it.



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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131816 - 01/06/21 09:49 PM (3 years, 1 month ago)

Quote:

Uzimyco said:
Streaks were done using sterile disposable innoc loops individually packaged. In SAB.

Agar PC at 15 psi for 30+ mins.  Poured allowed to cool in SAB.

ISO cleaned - uvc irradiated. Enough ozone to kill a horse. Spores protected from uvc naturally. Don’t know how anything could live through it?




This is the first time I hear of a disposable inoc loop, will take your word for it they're sterile, but I'd rather have a non-disposable loop that I can flame myself to ensure it's sterile every time.

All that about uvc and ozone and spores protected from uvc naturally is confusing the hell out of me. I honestly don't know what you're talking about.


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OfflineAlsetAlokin
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131844 - 01/06/21 10:01 PM (3 years, 1 month ago)

After innoc from spores nothing happens on days 1-3. That's probably why ya can't find anything.

Germination usually happens between day 5-14 IME.

You got some bacterial blotches there, give it some time. Myc might show up in a week.


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InvisibleDoctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131845 - 01/06/21 10:01 PM (3 years, 1 month ago)

I've seen people use UV lights for sterilization but I dont know if it's a novelty thing or what. Never entertained the thought of it. Not sure if that's what he's referring to.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131853 - 01/06/21 10:04 PM (3 years, 1 month ago)

Pics of sterile disposable innoc loops and 200nm uvc.

Uvc kills anything with dna. Mechanically. No way to become tolerant. Bulbs produce a lot of ozone. Helps to disenfect. I use a dosimeter. Confirms death to all. Enough uvc would kill any and every living thing. It is the sledgehammer of radiation



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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27131860 - 01/06/21 10:06 PM (3 years, 1 month ago)

Not a novelty in the least. Hospitals and food prep spend about 6 billion a year in uvc lighting. Not a joke. Been around for decades. Used in medical facilities. Air purification systems. Aquarium systems. Death is most surely guaranteed.

The earths ozone layer is the only thr that keeps us all dying from uvc. Cause immediate destruction at the dna level. Not to played with as it’s not safe. Need to
Cover and get the hell out of the room


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