Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Mix   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Offlinedbdbdb
Stranger
Registered: 05/24/20
Posts: 21
Last seen: 2 years, 11 months
Requesting Help with Inoculation Problems
    #27131093 - 01/06/21 05:24 PM (3 years, 22 days ago)

Hi!  Lately, I've had to toss a lot of rye wheat berry inoculation bags and any insight into what I'm doing wrong would be very helpful.

Strain
Melmack Reverted

Problem
Agar dropped into an inoculation bag either doesn't take or takes, but then fails to spread after a shake-up.

Tek
Rye wheat berries soaked in water for 21-24 hours.
Brought to a boil, laid to dry.
5lb in a plasic bag with 1" hole covered with breathing tape.
90m in PC at 11 PSI (best I can do)
Let cool overnight.

Drop agar slice into bag in SAB.
Seal with tape and set aside.

Outcome 1: Agar dries out and nothing takes.
Outcome 2: Myc forms.  10 days in, I shake and let sit.  Check back in a few days and looks diseased.


I think the problem is proper hydration of the wheat berries and/or bursting.  When exposed to air after the shake up, the burst berries mold and such.

In the first two bag pics, you see the myc actually backing AWAY from where it had originated with a yellowish ring around it.

Pictures



Extras: Filter Print Post Top
OfflineBallzagna
Carb Daddy
Male


Registered: 10/09/19
Posts: 619
Last seen: 13 days, 12 hours
Re: Requesting Help with Inoculation Problems [Re: dbdbdb]
    #27131221 - 01/06/21 06:07 PM (3 years, 22 days ago)

in the first bag pic, the left section is myc the right section is mold.

90 min @ 11psi probably wasn't enough... I do 2 hours at 16-17psi. Some people do 90min @ 16psi for smaller grains but oats and such can be pretty grody so I always do 2 hours. Also when using bags instead of jars that's a lot more volume to sterilize so the heat/steam might not have penetrated to the center of the bag as well as it would have jars.

I don't use spawn bags but you might want to look into buying premade ones or a better tek for making them yourself, I don't think micropore tape is a good filter for PC'ing grains, it works for no-pour agar plates because of the low PC time but I don't see it working well for anything else.


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs




Extras: Filter Print Post Top
Offlinedbdbdb
Stranger
Registered: 05/24/20
Posts: 21
Last seen: 2 years, 11 months
Re: Requesting Help with Inoculation Problems [Re: Ballzagna]
    #27131246 - 01/06/21 06:16 PM (3 years, 22 days ago)

In my first grow with this method, I made 10 bags and had to toss 2.  In this grow, I am following the same tek and made 6 bags and through away all 6.

Your comments make sense as my smaller mason jars generally don't have this problem (until the myc reaches burst grains at the bottom).  I'll ditch the bags for inoculation and switch to jars and use smaller fruiting containers.

Thank you for your help.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Mix   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* innoculation problem? ParticleMan 813 3 02/19/02 02:10 AM
by Anno
* How long after pouring agar do I have to wait before inoculation? Benson 4,953 19 01/06/21 02:10 PM
by Smelltheflowers
* A puzzling Problem. logicalMADNESS 1,182 11 11/12/02 04:18 AM
by On_the_Down-Low
* Problems Encountered During Inoculation sillvyr 1,441 8 01/23/02 11:29 AM
by sillvyr
* problems with grain quarts RedHead 3,574 5 04/02/02 07:37 PM
by RedHead
* Anyone have link or pics of early stage agar inoculation?
( 1 2 3 4 5 all )
Uzimyco 1,153 90 01/15/21 03:51 AM
by Blackrainbow2
* Sterilisation Problem
( 1 2 all )
QuebecShroomer 1,311 32 03/17/03 08:56 AM
by eoPh
* 30 jars w/ same problems?? pussycontrol 1,783 12 02/07/12 07:47 AM
by grainbrain

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
59 topic views. 28 members, 220 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.