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DigDug
Cultivator


Registered: 05/05/20
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Reviving refrigerated agar cultures
#27130568 - 01/06/21 01:58 PM (3 years, 22 days ago) |
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Hello, So I am a novice at cultivating but this is something I've never done. I refrigerated about 12 agar cultures for 6 months and now I'd like to grow them out. I would like to know how you guys go about doing this.
Does it work to put the older agar cultures directly to grain or should I do some transfers to more agar to ensure they're clean? What have you guys done in the past and how has it worked for you? I am happy after looking at them and noticing no bluing or contaminants.
I realize the inside of a fridge is pretty dirty so I have them wrapped in saran wrap.

All of your input is appreciated. Thanks
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Marxcelium
Mushroom Instrumentality Project

Registered: 05/12/20
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Re: Reviving refrigerated agar cultures [Re: DigDug]
#27130820 - 01/06/21 03:37 PM (3 years, 22 days ago) |
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I always transfer once to make sure the culture is clean after taking a plate out of cold storage. Condensation tends to build in my stored plates and can sometimes pool, so I just want to make sure everything is good before making a grain master with it.
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Messiah of Savants
Shaman



Registered: 07/02/08
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Re: Reviving refrigerated agar cultures [Re: Marxcelium]
#27131406 - 01/06/21 07:11 PM (3 years, 22 days ago) |
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It's probably not a bad idea to make a transfer out of the fridge petri like Marx mentioned, but I've had a lot of success simply taking them out of the fridge and putting them to straight to grains. I store them in stacked groups in gallon ziplock bags and put them in/take them out in front of the flow hood.
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Tstone
St.of Circumstance



Registered: 10/16/18
Posts: 944
Loc: This Planet
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6 months is kinda pushing limits. Biggest concern is if agar dries out. Myc can also go permanently dormant, if refrigerated too long. I usually make a habit of doing transfers every 3-4 months, just to keep the cultures viable, wake them up, transfer,let my plates grow out, back in fridge ( I transfer cold myc to new agar)I have several that are over 2 years old, but transfered 3-4 times a year, plus you get additional plates when you do transfers, one plate turns into 6,lol
Just be certain you check on them occasionally, agar does dry out.
I go directly from fridge to grain, however, I'm 100% certain they are clean.
I would transfer those, see if they are even viable.
-------------------- Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"
Edited by Tstone (01/06/21 08:28 PM)
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DigDug
Cultivator


Registered: 05/05/20
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Re: Reviving refrigerated agar cultures [Re: Tstone]
#27131620 - 01/06/21 08:17 PM (3 years, 22 days ago) |
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Thanks for all the input, I’ll make some transfers in the morning and let you all know how it turns out. I hope they show some signs of growth. Should know in a couple days.
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DigDug
Cultivator


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Re: Reviving refrigerated agar cultures [Re: DigDug]
#27143176 - 01/12/21 05:03 AM (3 years, 16 days ago) |
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Just wanted to give an update..
I transferred the 6 month refrigerated cultures to new agar and they are showing new signs of growth.

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savan73
Stranger
Registered: 05/05/20
Posts: 252
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Re: Reviving refrigerated agar cultures *DELETED* [Re: DigDug]
#27143224 - 01/12/21 06:11 AM (3 years, 16 days ago) |
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Post deleted by savan73
Reason for deletion: .
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RoadAppleSnapple
Learning.


Registered: 12/31/20
Posts: 58
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Re: Reviving refrigerated agar cultures [Re: Tstone]
#27143234 - 01/12/21 06:25 AM (3 years, 16 days ago) |
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Quote:
Tstone said: 6 months is kinda pushing limits. Biggest concern is if agar dries out. Myc can also go permanently dormant, if refrigerated too long. I usually make a habit of doing transfers every 3-4 months, just to keep the cultures viable, wake them up, transfer,let my plates grow out, back in fridge ( I transfer cold myc to new agar)I have several that are over 2 years old, but transfered 3-4 times a year, plus you get additional plates when you do transfers, one plate turns into 6,lol
Just be certain you check on them occasionally, agar does dry out.
I go directly from fridge to grain, however, I'm 100% certain they are clean.
I would transfer those, see if they are even viable.
I might diagree that 6 months is pushing the limits of a plate.
Total newb here. But, I just got back into mycology after 2.5 years. I saved myself some presents in the fridge, totally forgot about them -- I had the door taped shut.
I am getting real nice growth in a jar from a dried plate of Mexicana. Both my SemiL plates were happy, still moist, knotted, and one's jarred. Just plates taped and ziplocked.
I would transfer those first though as well OP, but I would definitely not give up if they are important to you. Good luck!
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jcm4620
Stranger


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i simply just take them out of the fridge and let them wake up for 3-4 days b4 doing anything with them. i notice after i pull my shit out it wakes up with a vengence and ready to eat.
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DigDug
Cultivator


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Re: Reviving refrigerated agar cultures [Re: savan73]
#27143733 - 01/12/21 12:10 PM (3 years, 16 days ago) |
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Quote:
savan73 said: How many degrees is your fridge? (fahrenheit)
35 Degrees
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Land Trout
Stranger



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Re: Reviving refrigerated agar cultures [Re: DigDug]
#27143849 - 01/12/21 01:08 PM (3 years, 16 days ago) |
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I had to take a break for almost a year, and had my plates in the worst fridge you could imsgine for 11 months. The fridge would go from freezing to room temp, it was also used as my bait fridge, so it also held live worms in containers right next to the zip locks that the plates were in. There was even visible mold inside the fridge. I had put three cubensis plates straight to grain after 11 months in there, also made new plates out of the best looking one. Two of those three old plates grew mold on the grain. But the one did fine, and just got my second flush from it. I threw maybe a dozen old plates away that just looked bad. But things are up and rolling once again, and I’m glad I tried them out, instead of starting from scratch. And now have dozens of plates from the one I made transfers from. Also had several ps. Subaruginosa, ps. Cyanescens, ovoid, oyster and gym plates. All the gym and a couple oyster plates were trash, they turned to goo. But the rest of the wood lovers are rocking. So six months in a good setup shouldn’t be a problem from my experience.
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Tstone
St.of Circumstance



Registered: 10/16/18
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That's a chance, personally, I'm not gonna keep my fingers crossed with a prize culture after more than 6 months in the fridge, on agar. Just me perhaps.
-------------------- Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"
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EZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
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Re: Reviving refrigerated agar cultures [Re: Tstone]
#27144345 - 01/12/21 06:15 PM (3 years, 16 days ago) |
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Has anybody else noticed a distinct change in growth pattern from dishes taken out of the fridge? Or does it just resume the same look of growth as it "wakes up" when you take yours back out?
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Land Trout
Stranger



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Re: Reviving refrigerated agar cultures [Re: EZPZyaBZ]
#27144562 - 01/12/21 07:43 PM (3 years, 16 days ago) |
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Those ones I was talking about were a lot more tomentose than when I put them away and took a couple transfers to get it back to where it was. They were really ropey but slow on the grain, But those were under pretty extreme conditions. Normally when they’re just stored for a couple months under better conditions they don’t change much. I used to work out of state for three months every summer, and they were always just fine when I’d come back.
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