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Offlineataribravo
Magic Myc
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Registered: 08/07/20
Posts: 75
Last seen: 2 years, 11 months
Stalled tub colonization
    #27126661 - 01/04/21 05:12 PM (3 years, 1 month ago)

My last dozen tubs or so have stalled at around 50% colonization. Here is the process up to this point:

Fully colonized rye grain jars (Purple Mystic from agar to jars) into straight coir and vermiculite (2 quarts per coir brick added) and mixed (not layered). Substrate is made by the modified bucket method, 2 bricks coir and 4 quarts verm with 2 gallons boiling water places in an insulated ice chest, stirred twice and left to cool (approx 6 hours or overnight). I am using 3 quarts of grain and 9 quarts of sub, giving me about a 3:1 ratio of sub to grain.

The monotubs are 32quart Sterilite gasket tubs with 2 1-inch holes on each side (about an inch above the top of the substrate) and 1 1-inch hole on each end (about 5 inches above the substrate or 1 inch from the top). The side holes are covered with 2 inch masking tape and the end holes are covered with 3 layers of micropore tape. The lid is set aside and foil is placed on the top with a 1/4 inch hole poked into each corner about 2 inches from each side.

The room is lit on a timer for 8 hours on - 16 off with 6500k LED lighting tubes. The temperature is kept between 68-72 degrees F.

The growth is a combination of rhizomorphic and tomentose and is very clean, but two weeks into colonization is just slows to a crawl.

Thoughts:
1. Too much or not enough FAE
2. Not a high enough sub to grain ratio
3. Not enough nutrients without some kind of manure

I have had success in this room with this setup before and can't figure out why I am hitting this brick wall. Any help is greatly appreciated!!



After adding these photos, I think my problem is not getting the substrate to field capacity. The bins are simply drying out. Thoughts?


Edited by ataribravo (01/04/21 08:30 PM)


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Stalled tub colonization [Re: ataribravo]
    #27126696 - 01/04/21 05:30 PM (3 years, 1 month ago)

pics always help, after 2 weeks u should be into fruiting


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineataribravo
Magic Myc
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Registered: 08/07/20
Posts: 75
Last seen: 2 years, 11 months
Re: Stalled tub colonization [Re: cronicr]
    #27127056 - 01/04/21 08:30 PM (3 years, 1 month ago)

I added photos. I think the bins look dry.


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Offlineataribravo
Magic Myc
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Re: Stalled tub colonization [Re: cronicr]
    #27130301 - 01/06/21 12:33 PM (3 years, 1 month ago)

Bump...


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OfflineMarxcelium
Mushroom Instrumentality Project

Registered: 05/12/20
Posts: 127
Loc: The Tharsis Bulge
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Re: Stalled tub colonization [Re: ataribravo]
    #27130321 - 01/06/21 12:39 PM (3 years, 1 month ago)

I personally can't tell why it's stalled from the photos, but, in my experience, when a tub stalls a this stage it's cause trich is about to take the stage. You could've let it dry out on top as well cause myc won't colonize bone dry coir.

I am curious why you've covered the top of the tub with foil when it seems like your light source is also coming from the top.  This won't cause the stalling, but do you want all your light bouncing back up to the ceiling away from your sub?


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Offline0ptiquest
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Re: Stalled tub colonization [Re: ataribravo]
    #27130334 - 01/06/21 12:42 PM (3 years, 1 month ago)

Probably just need more FAE, but why the foil lid? Also 2 weeks is not stalled yet i think.

The tape outside the tubs is doing nothing, people use liners (trash bags) for some reasons but light is not one of them.

Personally i dont bother using liners and dont have any sidepin problem, you just have to clean the tub more for the next use.


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: 0ptiquest]
    #27130352 - 01/06/21 12:50 PM (3 years, 1 month ago)

I feel like with just foil covering the top and with holes in the foil he should be getting enough GE to at least get the thing colonized.

Two weeks is pretty long for it to look like this unless the culture is incredibly sluggish, but what do I know.

I have these tubs, and, at the very lest here, I'd strip the gasket out of the lid and put the lid on.  This foil biz seems iffy.

If the coir is dry, spray the top and see what happens.  My oil heater in my grow room freaked out last month and baked the whole room to 100 degrees overnight, which dried the tops of my subs out so bad that I had to re wet them, but they all pulled through and at least flushed twice.


Edited by Marxcelium (01/06/21 12:53 PM)


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Offline0ptiquest
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130369 - 01/06/21 12:56 PM (3 years, 1 month ago)

When i started and make my tubs too wet with bad FAE i had to wait 18 days and even more to see pins, maybe its bacterial also but it looks a bit wet for me, the good thing is i have had tubs like that and they fruited well after all.


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OfflineDr.Sparkle
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Re: Stalled tub colonization [Re: 0ptiquest]
    #27130385 - 01/06/21 01:02 PM (3 years, 1 month ago)

Take a few layers of tape off? I am not sure on micropore tape. Search the forum for micropore dialed tubs, I’d say its time they need 1/2 layers for more FAE. Up the light on to 12hrs on.

I second put the lids on and take gasket off.

As above poster said taping the outside won’t do shit. If your worried about side pins get your tubs dialed in and surface conditions on point is the way to go.  Some people like them some don’t. They don’t help with poor surface conditions, tubs will still side pin with poor surface conditions.

2 bricks coir 4 verm. With 2 gallons of water sounds about right. 650g coir 3qt verm and 4.5ltr water is a good baseline.


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: Dr.Sparkle]
    #27130425 - 01/06/21 01:12 PM (3 years, 1 month ago)

FAE won't become a factor until they start fruiting.  To colonize, all you need is a little gas exchange so the colony doesn't stall out. I understand that giving the tubs proper FAE ("fruiting conditions") from the beginning will make the sub pin faster, but I really don't think it's stalling because of a lack of gas exchange if he's only got the lid covered in foil with holes poked in it.

Also, the black tape won't do anything, but it isn't hurting anything either, so it isn't helping the OP's issue to keep bringing it up.  It's fine where it is.


Edited by Marxcelium (01/06/21 01:15 PM)


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Offline0ptiquest
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130468 - 01/06/21 01:24 PM (3 years, 1 month ago)

In my opinion FAE is the bigger pinning factor, too wet it wont pin.


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: 0ptiquest]
    #27130488 - 01/06/21 01:29 PM (3 years, 1 month ago)

Right, but the tubs aren't even fully colonized in the pictures, so we arent worried about pinning just yet.


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OfflineDr.Sparkle
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130493 - 01/06/21 01:31 PM (3 years, 1 month ago)

Hang on shit it does look a little dry sorry I missed that I thought he was worried it was too wet. so I am wrong increasing the fae will dry the shit out further.


Edited by Dr.Sparkle (01/06/21 01:56 PM)


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: Dr.Sparkle]
    #27130535 - 01/06/21 01:46 PM (3 years, 1 month ago)

Just be careful becuase, if the problem is, as you suspect, OP, that your sub is too dry, increasing FAE, especially before the sub is fully colonized, will make the problem worse and not better unless you add moisture.


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OfflineDr.Sparkle
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130580 - 01/06/21 02:01 PM (3 years, 1 month ago)

OP is that a US or imperial gallon? If its us I would say that it probably did start off a little on the dry side. 4.5ltr per brick and verm might be a good bet. Double check your field capacity before spawning if its dry just add hot tap water, too wet get squeezing!


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: Dr.Sparkle]
    #27130634 - 01/06/21 02:19 PM (3 years, 1 month ago)

I also like to pasteurize some verm in the oven and then add it to coir if I overshoot the water to get it to field capacity.  It's easier for me than squeezing, but either works.


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OfflineDr.Sparkle
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130651 - 01/06/21 02:23 PM (3 years, 1 month ago)

That sounds better than squeezing, squeezing does suck. I in the end just did a bunch of tests and found out which ratio was best for me. So now I can just add water and I am done. I use hot tap water now and have gone away from boiling and coolers. I find it actually works better and saves so much time. Eats unbucket tek inspired me to try.


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OfflineMarxcelium
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Re: Stalled tub colonization [Re: Dr.Sparkle]
    #27130668 - 01/06/21 02:29 PM (3 years, 1 month ago)

Yeah, I always think about doing it Eat's way and chicken out at the last minute in favor of the old bucket tek.  I should knuckle down and give the simple way a spin.


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OfflineDr.Sparkle
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Re: Stalled tub colonization [Re: Marxcelium]
    #27130773 - 01/06/21 03:14 PM (3 years, 1 month ago)

I felt the same. Scared the shit out me, wouldn’t go back now. Swear the myc prefers it, haven’t done any side by sides just what I see. There is some coir I have seen I definitely would not do it with though! Although that shit I wouldn’t use either way now anyway.


Edited by Dr.Sparkle (01/06/21 03:19 PM)


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Offline0ptiquest
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Re: Stalled tub colonization [Re: Dr.Sparkle]
    #27130844 - 01/06/21 03:48 PM (3 years, 1 month ago)

Does it have a top layer? The substrate doesnt need to be all white to be ready to fruit, i think it will pin soon just wait. More FAE wouldnt hurt.


Edited by 0ptiquest (01/06/21 03:50 PM)


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