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Offlinehahasauce
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First contam experience?
    #27130033 - 01/06/21 10:35 AM (3 years, 22 days ago)

Hey guys so harvested a solid 60g dry from my 4 shoeboxes all were nice and fine yet within the past few hours this popped up in one of them, I'm assuming this is the first signs of trich as I began to be quite lazy with sterile techniques while harvesting (lesson learned) I guess I was a bit too confident with the myc having a solid footing on the substrate for future flushes. I am also quite sure this is a tub I harvested this morning without gloves for the first time (so reminder to always be sterile!!)

Can anyone identify what kind of mold/contam this may be? I have isolated it and will let it go for the night just to see how it turns out for future reference





Edited by hahasauce (01/06/21 10:58 AM)


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Offlinehahasauce
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Re: First contam experience? [Re: hahasauce]
    #27130083 - 01/06/21 10:55 AM (3 years, 22 days ago)

Also just want to add that I'm not looking for solutions to salvage this (although I'm praying it won't happen with my other tubs) - I'm sure 99% of you would just simply want to throw it in the trash on first sight but I am looking at it as a learning experience as I know this hobby won't always be fun & games!!


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OfflineCrackatoa
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Re: First contam experience? [Re: hahasauce]
    #27130086 - 01/06/21 10:57 AM (3 years, 22 days ago)

Looks like trich from the pictures, thick white turning green??


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Offlinehahasauce
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Re: First contam experience? [Re: Crackatoa]
    #27130088 - 01/06/21 11:01 AM (3 years, 22 days ago)

Quote:

Crackatoa said:
Looks like trich from the pictures, thick white turning green??




I was looking for signs of green and although I suspected it at first, I'm not 100% sure. One thing I can say for certain is that it has spreaded RAPIDLY - this was not there whatsoever last time i looked approx 5hrs ago.

I do think this was a tub I harvested this morning (approx 5hrs ago) without sterile techniques so I may have introduced it myself - I did a terrible job of harvesting while also disturbing the substrate as well as not using gloves


Edited by hahasauce (01/06/21 11:02 AM)


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Offlinehahasauce
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Re: First contam experience? [Re: hahasauce]
    #27130098 - 01/06/21 11:05 AM (3 years, 22 days ago)

My main question really I suppose is Trich a contam that is commonly transmitted in an airborne manner, meaning if I open it up near other tubs are they at risk - or is it a physical thing where either endospores have remained in the substrate and finally been given a chance either from diminishing healthy mycelium conditions (FAE, Humidity) or could it possibly be by physical contact from the grower


Edited by hahasauce (01/06/21 11:08 AM)


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InvisibleMelliferous
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Re: First contam experience? [Re: hahasauce]
    #27130164 - 01/06/21 11:28 AM (3 years, 22 days ago)

Quote:

hahasauce said:
My main question really I suppose is Trich a contam that is commonly transmitted in an airborne manner, meaning if I open it up near other tubs are they at risk - or is it a physical thing where either endospores have remained in the substrate and finally been given a chance either from diminishing healthy mycelium conditions (FAE, Humidity) or could it possibly be by physical contact from the grower



Trich is an airborne contaminant.  A healthy tub exposed to room air is therefore exposed to small quantities fo Trich spores on a regular basis. Coco coir and healthy mycelium both resist Trich colonization. Most Trich contamination before first flush is due to a problem with spawn not being clean or a really dirty spawning process (dirty hands, etc.). Low FAE and high humidity can also contribute to giving contaminants an upper hand.


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Edited by Melliferous (01/06/21 11:29 AM)


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Offlinehahasauce
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Re: First contam experience? [Re: Melliferous]
    #27130181 - 01/06/21 11:37 AM (3 years, 22 days ago)

Quote:

Melliferous said:
Quote:

hahasauce said:
My main question really I suppose is Trich a contam that is commonly transmitted in an airborne manner, meaning if I open it up near other tubs are they at risk - or is it a physical thing where either endospores have remained in the substrate and finally been given a chance either from diminishing healthy mycelium conditions (FAE, Humidity) or could it possibly be by physical contact from the grower



Trich is an airborne contaminant.  A healthy tub exposed to room air is therefore exposed to small quantities fo Trich spores on a regular basis. Coco coir and healthy mycelium both resist Trich colonization. Most Trich contamination before first flush is due to a problem with spawn not being clean or a really dirty spawning process (dirty hands, etc.). Low FAE and high humidity can also contribute to giving contaminants an upper hand.





Thank you so much for the info!

as mentioned, this occured after my first flush so I'm quite confident it didn't have much to do with the spawn itself. If my other tubs don't show any signs of contam then I will assume it was from me being sloppy when I thought all the hard work was done. Need to recognize that it's a walk/run and not a sprint to the finish line when it comes to this craft


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OfflineMarxcelium
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Re: First contam experience? [Re: hahasauce]
    #27130211 - 01/06/21 11:54 AM (3 years, 22 days ago)

I don't know man, if this popped up shortly after a first flush, it's probably that your spawn was bacterial, which opened the door for eventual trich colonization. A healthy substrate made with clean spawn won't get trich until it's flushed quite a few times, regardless of whether you've got some mold spores on your hand when you harvest or not. FAE is constantly carrying mold spores into your tubs anyway as stated above.

Clean spawn is what gives your sub the longevity to flush until its truly spent.

Don't sweat sterility too much after your tub is colonized. I keep tubs in my nasty garage in the summer months, harvest them all bare handed, and they flush great for weeks as long as they started clean.


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OfflineProfessor X
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Re: First contam experience? [Re: Marxcelium]
    #27130332 - 01/06/21 12:42 PM (3 years, 22 days ago)

I agree with clean spawn allows to flush until spent. I usually get a set amount of dry weight before trich hits it, usually after the 2nd or 3rd flush but I've already gotten a very good amount so I toss and replace. You are definitely dealing with trich and it was already there before you harvested. If you are working with MS move to agar. 60g dry from 4 shoeboxes is not a lot. 1.5 oz per quart is average with a good culture and clean healthy spawn 1st flush. I would imagine all your tubs probably have some bacteria or yeast hiding. Either will stall the mycelium after harvest and let the trich take hold. Also when your mycelium is producing metabolites trying to fight invaders it's wasting energy it can use to reproduce. Good luck on your next run!


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Offlinehazyhorse
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Re: First contam experience? [Re: Professor X]
    #27131018 - 01/06/21 04:53 PM (3 years, 22 days ago)

don't let contam'd subs sit too long, the longer they sit the more spores they produce which can really start fucking your shit up if you aren't very conscientious of how you dispose of them


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Offlinehahasauce
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Re: First contam experience? [Re: hazyhorse]
    #27132232 - 01/07/21 05:50 AM (3 years, 21 days ago)

thanks guys. took another look today and definitely some green showing. FORTUNATELY, other 3 tubs arent showing any bad signs and have started pinning again after I gave them each a dunk.

someone said 60g dry not good for first flush but as this is my first time i disagree im over the moon with it and consider the learning experience alone with the effort. Already have about 1.5kg of more spawn ready to go so onto the next one while I learn about cloning/agar more :smile:


Also i will dispose of the contam sub immediately, thanks for the tip


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OfflineCrackatoa
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Re: First contam experience? [Re: hahasauce]
    #27132283 - 01/07/21 06:39 AM (3 years, 21 days ago)

Exactly, be happy with 60g. So many start down this road and end up with nothing on their first try, sometimes even their second go around. Seen several give up without ever getting a fruit.


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