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OfflineUzimyco
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Anyone have link or pics of early stage agar inoculation?
    #27129824 - 01/06/21 07:24 AM (3 years, 22 days ago)

Hi All

I finally got my agar going and of course there are countless pics of well grown and colonized agar available both on this site as well as the internet in general.

I have been unable to locate any really early development pics for example, illustrating what onset type germination looks like.

My dishes show a varied degree of activity and started to with 1-3 days of spore inoculation.  Due to some less than perfectly exacting innoc. techniques (smears here, better zig zag there) the dishes exhibit fairly different looking characteristics.  Since this is really a first time agar run it is difficult to discern if I’m seeing things positive or negative.  Again if it was clearly a thick grown out plate of myc then yes it would be more obvious. I’m watching daily as I’m trying to learn any patterns or behaviors however without a frame of reference at this point, I couldn’t say if I’m growing mycelium or if I’m growing influenza :crazy:

Any such info or links would be most appreciated.

Thank you in advance!!


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27129830 - 01/06/21 07:30 AM (3 years, 22 days ago)

https://www.shroomery.org/forums/showflat.php/Number/25662166#25662166

This might be the thread your're looking for.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131690 - 01/06/21 08:54 PM (3 years, 22 days ago)

I appreciate the link but so many of those look like transfers to
Me? Not necessarily early onset spore growth?

Maybe I should post some pics and can get some advice. I’m not
loving what it looks like though currently.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131716 - 01/06/21 09:04 PM (3 years, 22 days ago)

Here are some pics. I swear I don’t know what the hell I’m looking at here.




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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131729 - 01/06/21 09:09 PM (3 years, 22 days ago)

I'm afraid there's only bacteria on all plates. But in the third pic there seems to be a little patch of mycelium that looks like it could grow into something you could take a clean transfer from. What was your process? What did you use to inoculate your plates?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131734 - 01/06/21 09:11 PM (3 years, 22 days ago)

Plates 1 to 5 are contaminated with bacteria.
I imagine bacteria will begin to show on plates 6 and 7, unless it has and my eyes are dogshit right now.

That bacteria follows your streaking so somethings up with your technique or worse here.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: starbones] * 1
    #27131748 - 01/06/21 09:17 PM (3 years, 22 days ago)



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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27131769 - 01/06/21 09:27 PM (3 years, 22 days ago)

Don’t know if it’s against the rules?

Any experts willing to give some limited direct instruction?

That means I pay for say 15 mins on the phone... vid chat etc?  My platform allows for me to give you a site address and a code. No download on your side. Just hit the site - enter the code - were talking.

Cant fathom how it’s Possible. Zip you over $50-$100 for 15 mins. Clear my issues once and for all.

Any taker ? Or is that a gargantuan no-no?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131781 - 01/06/21 09:35 PM (3 years, 22 days ago)

:lol: That's a new one. Never seen nobody asking to talk on the phone to someone to fix their agar issues.

Man we do this everyday here on the forum, helping people overcome their issues. I think nobody is going to want to call you. :cop:

Tell us your process and I'm sure we can lend you a hand.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131786 - 01/06/21 09:39 PM (3 years, 22 days ago)

Streaks were done using sterile disposable innoc loops individually packaged. In SAB.

Agar PC at 15 psi for 30+ mins.  Poured allowed to cool in SAB.

ISO cleaned - uvc irradiated. Enough ozone to kill a horse. Spores protected from uvc naturally. Don’t know how anything could live through it?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131793 - 01/06/21 09:40 PM (3 years, 22 days ago)

You used a print?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131797 - 01/06/21 09:42 PM (3 years, 22 days ago)

Damn, we should start a mush cult call center. I'd do it for free but my house is shut down for the night. Everyones asleep except for me cause I'd rather hang out with you guys and hate myself when I have to get up for work in the morning.

Spores can take as long as 3 weeks to germinate. When they do, it'll look like little white hairy spots. In that link that I shared, you'll see a blue plate with a Z pattern that has little white areas all around the spore clumps. Those are what you're looking for.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131799 - 01/06/21 09:43 PM (3 years, 22 days ago)

Well I agree. Sounds a bit weird. But I don’t see an issue with the transfer of info. Vids all over you tube and like I said. Could go web based with no client. I know the technology. I sell it for a living.

Just figure I don’t expect anyone’s time and experience for free. Don’t mind paying for advice personally but I get it sounds weird. Like I said. Just frustrated with running into trouble over snd over


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131807 - 01/06/21 09:46 PM (3 years, 22 days ago)

Yes green from b+ print. Blue from GT print. But I want suggesting right now anyway. At a time that was convenient for someone.

I work with consultants in business everyday. It’s normal for me although not the same subject matter but that was my thought process.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131809 - 01/06/21 09:47 PM (3 years, 22 days ago)

That looks like myc. Wouldn't hurt to watch it and transfer before the bacteria gets to it.



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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131816 - 01/06/21 09:49 PM (3 years, 22 days ago)

Quote:

Uzimyco said:
Streaks were done using sterile disposable innoc loops individually packaged. In SAB.

Agar PC at 15 psi for 30+ mins.  Poured allowed to cool in SAB.

ISO cleaned - uvc irradiated. Enough ozone to kill a horse. Spores protected from uvc naturally. Don’t know how anything could live through it?




This is the first time I hear of a disposable inoc loop, will take your word for it they're sterile, but I'd rather have a non-disposable loop that I can flame myself to ensure it's sterile every time.

All that about uvc and ozone and spores protected from uvc naturally is confusing the hell out of me. I honestly don't know what you're talking about.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131844 - 01/06/21 10:01 PM (3 years, 22 days ago)

After innoc from spores nothing happens on days 1-3. That's probably why ya can't find anything.

Germination usually happens between day 5-14 IME.

You got some bacterial blotches there, give it some time. Myc might show up in a week.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131845 - 01/06/21 10:01 PM (3 years, 22 days ago)

I've seen people use UV lights for sterilization but I dont know if it's a novelty thing or what. Never entertained the thought of it. Not sure if that's what he's referring to.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131853 - 01/06/21 10:04 PM (3 years, 22 days ago)

Pics of sterile disposable innoc loops and 200nm uvc.

Uvc kills anything with dna. Mechanically. No way to become tolerant. Bulbs produce a lot of ozone. Helps to disenfect. I use a dosimeter. Confirms death to all. Enough uvc would kill any and every living thing. It is the sledgehammer of radiation



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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27131860 - 01/06/21 10:06 PM (3 years, 22 days ago)

Not a novelty in the least. Hospitals and food prep spend about 6 billion a year in uvc lighting. Not a joke. Been around for decades. Used in medical facilities. Air purification systems. Aquarium systems. Death is most surely guaranteed.

The earths ozone layer is the only thr that keeps us all dying from uvc. Cause immediate destruction at the dna level. Not to played with as it’s not safe. Need to
Cover and get the hell out of the room


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131874 - 01/06/21 10:13 PM (3 years, 22 days ago)

Many thanks for this post. The kind of info I’m looking for.

Yes it’s been 3-4 days since inoculation.

I appreciate that input. I maintain my offer if anyone decides to chat with a goofball for 10-15 mins. Understand if no takers and I appreciate the help everyone is giving. I’m a science hobbyist snd I don’t like being unsuccessful. I’m usually not.

This confuses me. My new flow hood will be compelete in the next couple of weeks when the damn filter gets in. Has to be better than the box. Cant stand the SAB


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131877 - 01/06/21 10:16 PM (3 years, 22 days ago)

People usually work in a still air box or in front of a laminar flow hood. I've heard some professional hoods come with a bulb like that but you won't ever really need that for any sterile procedure in mycology.

That bacteria is located on the areas you streaked, which makes me think the inoculum (spores) was dirty, it's either that or the disposable loops, but I admit those look legit. The weird thing here is that you said you used a print and even sloppily made prints won't give ya that much bacteria unless they weren't allowed to dry properly. Are both prints from the same source? How old are they?

also;
Quote:

AlsetAlokin said:
After innoc from spores nothing happens on days 1-3. That's probably why ya can't find anything.

Germination usually happens between day 5-14 IME.

You got some bacterial blotches there, give it some time. Myc might show up in a week.




:whathesaid:


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131882 - 01/06/21 10:17 PM (3 years, 22 days ago)

Yes both prints from the same vendor.

Dated 11/20


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131887 - 01/06/21 10:21 PM (3 years, 22 days ago)

Only thing I can think is I used some distilled water. Boiled and put into beaker. First placed spores into cooled water and let sit covered in SAB (and uvcd as well).

Maybe should have just used dry spore???


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131892 - 01/06/21 10:23 PM (3 years, 22 days ago)

At any rate I think you have some myc in the third pic you posted. Mario circled it for you. You could transfer that to a new plate.

Quote:

Uzimyco said:
Only thing I can think is I used some distilled water. Boiled and put into beaker. First placed spores into cooled water and let sit covered in SAB (and uvcd as well).

Maybe should have just used dry spore???




Ok problem solved lol

Yea man, you seriously fucked up there.

Use dry spores, of course.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131898 - 01/06/21 10:26 PM (3 years, 22 days ago)

BINGO.

We found our winner, I was also puzzled at how wet those streaks looked.

DRY spores work fine!

If they're old and stubborn you can hydrate them using a swab maybe, but that shouldn't be necessary.

There's still hope though, granted you didn't use a liter of water to one small print there's probably some spores mating in those dishes as we speak.

The bacterial blotches are likely caused by your use of water. Try again with a dry print.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131911 - 01/06/21 10:31 PM (3 years, 22 days ago)

Ok got it.

I saved spores just in case so I’ll redo with dry.

Still don’t understand however how the water is the vector.  Boiled the shit out of it snd it went into a lab quality test tube. Sealed. Moved to SAB then poured into a sterile beaker. Covered beaker with sterile foil that was cleaned with iso and physan first?  Just sat there for about 6 hrs but can’t imagine how boiled distilled could be the culprit but don’t get me wrong. I’ll redo it tomorrow dry. Just confused about the water??


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131913 - 01/06/21 10:32 PM (3 years, 22 days ago)

Spores to agar

Here's some videos if you're more of a visual learner. None with dry spores to agar but you can reference the spore swab video in replacement of dry spores and an inoculation loop.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131916 - 01/06/21 10:34 PM (3 years, 22 days ago)

I was like how the hell did he get that much bacteria from a damn print?? Fuck that's even too much even for dirty spore solution lol
Even if you were to fold the print still wet I doubt it would give ya so much bacteria on agar, so it was obvious something else was up. Glad we found the last piece of the puzzle.


Edited by Josex (01/06/21 11:02 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131920 - 01/06/21 10:37 PM (3 years, 22 days ago)

Quote:

Uzimyco said:
Ok got it.

I saved spores just in case so I’ll redo with dry.

Still don’t understand however how the water is the vector.  Boiled the shit out of it snd it went into a lab quality test tube. Sealed. Moved to SAB then poured into a sterile beaker. Covered beaker with sterile foil that was cleaned with iso and physan first?  Just sat there for about 6 hrs but can’t imagine how boiled distilled could be the culprit but don’t get me wrong. I’ll redo it tomorrow dry. Just confused about the water??




Waters a vector, the pot it was in was a vector, the transfer of water to the tube is a vector, the iso is a vector, the foil is a vector.

Sometimes with this, the simplest way is the most sterile way and there's no need to complicate it,

The methods described in the TEKs here have been tested for years by hundreds of members, follow them and then you can deviate if you found a better way.


Edit: benefit of the doubt you could have all this extravagant equipment that could help you, but that print probably had some bacteria in it and you gave it a media and environment to multiply in. If you go dry spores to agar that myc will likely out compete the bacteria to a point where you can make a decent transfer.


Edited by AlsetAlokin (01/06/21 10:42 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131922 - 01/06/21 10:39 PM (3 years, 22 days ago)

UVC is def unnecessary. I've used loops like that at a clinical lab I used to work at. Its probably better to just use cottonswabs sterilized in foil in the PC. If you feel good about the print and your technique you can save that swab instead of going back to the print next time. But all that bacteria around the inoculation site means something's up. Usually that the inoculant is bacteria laden. Could come from condensation and mishandling of the plate, but have to keep your mind on many other factors in applied microbiology.

Edit: yeah, the water's unnecessary and not done correctly. Just boiling won't sterilize, have to pressure cook (similar to autoclaving). Just use a sterile swab and swab it on some firm agar.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131924 - 01/06/21 10:40 PM (3 years, 21 days ago)

Yea you did too many unneccessary steps, all of them huge vectors.


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OfflineUzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Josex]
    #27131949 - 01/06/21 11:07 PM (3 years, 21 days ago)

Understood

Thanks to all. I got thrown off by people saying “just make  a spore syringe snd drop a drop in the middle. Figured ok. But this way I could easily streak it.

Well - fuck up #10, #11?  Christ. Just frustrating.

Again much thanks to you all for your insight. I seem to have to learn this from endless mistakes. Just so much contradictory info out there. Hard to parse out correct and incorrect.

Thank you again genuinely. Tomorrow I’ll try again. Wonder if I should pressure clean my refrigerated plates first ?  Couldn’t hurt right?

-update-  agar was pc before poured. But why not re pressure cook while already in the dishes?  Not a bad idea right?


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Edited by Uzimyco (01/06/21 11:09 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131957 - 01/06/21 11:16 PM (3 years, 21 days ago)

You can't sterilize petris, they'll melt unless they're borosilicate petris and even then, the agar would boil over and they'll come out empty after the cycle. You can use those that are in the fridge no problems.

Incidentally, not the best idea to store blank plates in the fridge. If there's bacteria present due to an insufficent cycle or an user error when you poured them, you want it to grow so you know not to use those plates.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27131959 - 01/06/21 11:17 PM (3 years, 21 days ago)

Quote:

Uzimyco said:
Thank you again genuinely. Tomorrow I’ll try again. Wonder if I should pressure clean my refrigerated plates first ?  Couldn’t hurt right?

-update-  agar was pc before poured. But why not re pressure cook while already in the dishes?  Not a bad idea right?




If you have glass plates I've seen people sterilize them in ovens, most people here use pre-sterilized single use 100mm plates.

Use the "search posts" button in the banner up top and narrow your results accordingly. Try to use up to date information, if it's old there's a good chance we found a better way to do it.

Key words, search: glass petri, no pour, holy grail plates, agar - click the "trusted cultivators" box for sound advice, and be skeptical of most other (including myself) a lot of us are new here or only skilled at a few things.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27131983 - 01/06/21 11:48 PM (3 years, 21 days ago)

Many many thanks again. 

My dishes are all borosilicate glass but the over boiling of agar makes it a no go.


Illl try some refrig. Hate to make new agar 🧫 with 20 parafilmed plates.

Otherwise guess. It’s yet another do over.

I’m thankful for the experienced voices and advice. Many thanks to you all!


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Edited by Uzimyco (01/06/21 11:50 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27132311 - 01/07/21 07:14 AM (3 years, 21 days ago)

You're asking the right questions and maintaining a good attitude. I hope to see your future progress. Let's get you a rating.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27134079 - 01/07/21 09:04 PM (3 years, 21 days ago)

Thank you very much!  Again the advice is so genuinely appreciated!! :thumbup:

I admit, I’m not a fan of the internet for more reasons than I can list.. The forums of the internet, such as shroomery are one of the very few exceptions in my opinion, Information is shared purely out of the desire to teach and assist. I think there are few things on the internet that offer such value.

*I did plate 6 dishes tonight with dry spore as instructed and I took extra precaution to ensure I didn’t miss anything that I could disinfect.

Ironically as I went to empty the incubator, I noticed one plate that looked like it might be trying to out run something. Pic is below. I left it and 1 other in with the new plates as they looked like maybe something positive was brewing.  High hopes for this new batch.

Here is the one that looked like maybe it was something. If not, maybe something bad but I thought it was worth a bit if a grow out. With no real frame of reference it’s certainly a challenge to know what it is (the pics I posted yesterday were taken within minutes of posting so the one day certainly made some difference. This pic was from 30 mins ago). It’s not in great focus, there is glare from the lights and some overlapped parafilm. I don’t know if I could have possibly added any more detracting features to the photo if I tried.




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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27134162 - 01/07/21 10:10 PM (3 years, 21 days ago)

It's definitely hard to tell because of the low quality photo. It looks like myc but I've encountered mold that looks like that too.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27134707 - 01/08/21 08:27 AM (3 years, 20 days ago)

Agreed. No way to tell and certainly not by my naive eye.

I’ll keep you all updated and perhaps you can tell more in some time. The 6 additional plates I did with dry spore yesterday are a bit ridiculous. So much spore it looks like a race track on the agar, but I went with the “more is better” concept. Just figured at least if something grows out I can take a
Section and move on but I was definitely “heavy handed” to say the least.

Always thankful and appreciative for your experience snd assistance!!


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27135253 - 01/08/21 01:05 PM (3 years, 20 days ago)

Quote:

Uzimyco said:
Ironically as I went to empty the incubator




Your what?

Post some pics of your work space and people on here could help point out some flaws in your technique. (This is my favorite method to see if I'm fucking something up big time)

Don't cook the plates. Mold looooves heat, and I've had all of my plates do fine at 63°F 20-40%RH in a closet and now that is my preferred method.

Only take transfers from where you inoculated, those spots could be satellite colonies but it becomes difficult to differentiate when you use syringes or have a wet plate. Another reason dry spores are preferred.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27135978 - 01/08/21 06:18 PM (3 years, 20 days ago)

Hahaha 🤣  yes incubator is really an exaggerated term for sure.

It’s just an opaque Rubbermaid tub, that is floating in water which has an aquarium heater in it. Then above it has a couple of heat lamps shining down. The thermometer reflects 75 degrees virtually at all times. Being that im in south Florida, the air conditioner runs ALOT.

It is interesting what you’re saying though. I mean 63 degrees? Here in Florida you couldn’t get a house down to 63 degrees virtually ever. Now what’s really interesting is that as some time has gone on, I’ve been dialing back the temps (pre agar use).  It is hard to believe that when I began reading about the subject many things stated “best practices” of temperatures of 84-86 degrees.

I decided to dial it back on my own. It’s really strange though that so many things reference fairly high temps and then others refer to room temp snd even as low as you’ve suggested.

Pretty big spread to me. The disparity confuses me.....


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27136000 - 01/08/21 06:26 PM (3 years, 20 days ago)

so in other words you have them in a contamination chamber😂😂

as stated above good ol room temp is the best way. ur only askin for problems playin with useless toys


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136008 - 01/08/21 06:29 PM (3 years, 20 days ago)

Yeah, that contraption sounds crazy. And just not necessary. Gulf coast here and my plates are at room temp year round.

You're begging for contamination hombre


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27136026 - 01/08/21 06:39 PM (3 years, 20 days ago)

I'm no ethnobotanist, or mycologist, I just see what countless others have done on here and what has worked for me. It's usually plates in a closet, drawer, or rack.

70°F is the sweet spot for most things when it comes to cubes from what I've gathered.

Making things overbuilt is fine if that's your thing, but no one wants to clean a tank, replace water, bulbs or has the facilities to manage that. So collective this forum creates TEKs to simplify the entire process and achieve the best results.

If you look at TEKs made 10 years ago they're extremely complicated and overbought because that's the only way that was known how to do it. We know a lot more now.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: AlsetAlokin]
    #27136091 - 01/08/21 07:09 PM (3 years, 20 days ago)

Yeah, totally ditch that thing. Some recommend not letting anything get over 80°F because bacteria and mold thrive at those higher temperatures however, that's exactly what I want. If there's any unwanted shit in my plates/tubs I want it to show up sooner than later.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27136292 - 01/08/21 08:37 PM (3 years, 20 days ago)

i dont suggest ever letting plates get over 72-75deg f. there just simply no need for it and if u think u need to do that in order to see if somethin will show then i think you really need to pay closer attention to detail. quite honestly if you cant get a grain worthy plate by t2 or t3 by the latest you should prolly review your sterile technique cuz there is somethin your causing that is givin you the issues.

this is just a general statement here. its not directed at anyone. i know what ur sayin mario about wanting shit to show its self in early stages


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136306 - 01/08/21 08:44 PM (3 years, 20 days ago)

You're not wrong. I dont intentionally heat the area to bring the temp up but during summer it was pretty normal to see my grow area get up to 80°F. I just let it ride.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27136470 - 01/08/21 09:47 PM (3 years, 20 days ago)

ya i understood what u were sayin all the way man👍👍👍

my area stays at the 76-78 range perfectly i dont even have my heat vent open in the room and same in the summer with the air on. just the fcs lights and pumps runnin heats it perfectly for the temp im shooting for with pans


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136477 - 01/08/21 09:53 PM (3 years, 20 days ago)

I've got a couple fleuroescent tubes that keep the area warm. Just the top of the closet. During the summer 1/4 tubes were on and it got up to 82°F at the highest. Now that it's cold (this house is drafty) I keep 2 turned on and the temp stays around 75 during the day. I think it gets down to low 60's at night when the lights are off. Havent checked the thermometer in a while. I just read your pan tek. Definitely gonna be doin that in the future. Have a bunch of cubes I wanna cult first. Thinking about gourmet too just not sure if theres any that are plausible with my living situation. Don't want oyster spores in my house. Pan's will give me the straw pasteurization experience for when I make the jump to gourmet?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27136483 - 01/08/21 09:57 PM (3 years, 20 days ago)

ya i hate not havin the room/space for all i would like to grow😂


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136518 - 01/08/21 10:19 PM (3 years, 20 days ago)

Same. Gonna have to kick the wife and kids out to free up some rooms 😆


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136530 - 01/08/21 10:30 PM (3 years, 20 days ago)

I think I’m exactly where you guys are saying really.

Look at the thermometer in this pic. It’s right at 75. That’s room temp. Not hot anymore. I mean from what I’ve seen cubes grow in warm areas. Areas that don’t get to
65 from what I can tell.  They are mostly zone 10 and higher. 70-75 is on the low end of those zones. I’ve been plenty successful with cakes. Had some trouble with agar but it’s a first go and I think you’ve all determined that my water bath was bad and I didn’t sterilize it enough.

I’m thinking this dry spore run will be devoid of major contam. That was likely the error. We’ll know in a couple of days here. But again. I used to be at 85. Now at 74-75. Like I said. Cubes grow in warm places. Florida Brazil, Hawaii, Thailand. These are all zone 10 and higher. I’ve been a plant collector of tropicas for 30 years. I know at least what endemic temps are. I have to believe I’m ok at 70-75. Cakes at 85 blew up.

Not disagreeing. But my contam issue here wasn’t really temp related. The boiled water bath was just nowhere near sterile enough. Thank But I’ve cut that out. I’m hoping I’m good now.

The box serves as an additional sterile environment. Just keeps things out. It’s not fancy but it is certainly cleaner than being exposed in the room. Temp
Gauge is at the top if you can zoom in. Big number is humidity. Small is temp. I’ll dial it back a few



Plates on top are new. Dry spore. Bottom some of the left overs from the disaster. But we’ll see if that was the issue for sure.


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Edited by Uzimyco (01/08/21 10:33 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27136540 - 01/08/21 10:36 PM (3 years, 20 days ago)

well have fun playin with ur contamination station
sterilized water 😂 u can fill that thing with iso if u wanted and its still gnna work against your interest.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136543 - 01/08/21 10:38 PM (3 years, 20 days ago)

i store my plates in a fuckin mold ridden room with not pretty carpet on a shelf and i also fart, burp,cough,sneeze, smoke cigs, eat,drink and leave the occasional trash out and still zero issues. ur problem is ur over engineering a paper airplane🤷🏼

and im also willing to bet there is holes in ur prep either of the agar its self or in ur sterile technique



il just say one more time. ditch the mr wizard contamination propagation station. review your agar prep and sterile tech pour control plates ect. and work it out thru process of elimination. you do that and ul b just fine


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Edited by jcm4620 (01/08/21 10:47 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27136650 - 01/09/21 12:11 AM (3 years, 19 days ago)

I agree with you're overcomplicating things. I leave everything at room temp, was 72 forever but recently changed to 74 to make the wife happy. I grow in a carpeted room that also houses 5 rats and a dog. I do all my transfers in front of a flow hood one room over 3 or 4 feet from 7 adult male rats. I have a 95% success rate. I tried all the fine tuned mumbo jumbo, incubators etc... Big waste of time. I cycle 23 tubs totalling 54 quarts of spawn and don't have time for downtime, I get 2 flushes and chuck. 1 month life cycles. What I'm saying is there is a lot of good info on this site as well as good advice in this thread.

Some real solid advice, pay attention to dates on threads, if it's old it's probably outdated. When you see some crazy tek or info that looks too good to be true, read through to the end, if they didn't post results there is a reason for that. And stop following PF teks, that shit is super old and didn't work when he wrote it. Only reason I learned to grow mush 20 some odd years ago is because I have a high IQ and paid attention in biology.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Professor X]
    #27136980 - 01/09/21 07:45 AM (3 years, 19 days ago)

All understood guys.

Many thanks as always for the invaluable advice! I’m following all you’ve all laid out here now with much more confidence that I’m headed in the right direction.

I do admit - I’m still waiting on my hepa to complete my new hood. The SAB will be the death of me. I’m so looking forward to the pleasure of working in front of the flow hood vs the glove box. Everything is ready. My panels. My blower. Just need the damn filter. Was ordered back 3 weeks ago but all of these companies have like a 4-6 week lead time. There’s an opportunity to make some cash. Start making particle board box hepa filters. What a racket!?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137000 - 01/09/21 07:58 AM (3 years, 19 days ago)

I read your pan instructions as well. Really awesome. So we’ll written. Right to the point. No extraneous info.  I’m nowhere near trying it but if/when I do I’ll use your write up as an a-z guide.

I did laugh quite a bit as in that case you sound like me. I currently have (although have never used so far - all kinds of airstones, multi port pumps, timers with 1 minute intervals, etc). Since I’ve been into horticulture for so long I’ve become accustomed to the “gear” so without even knowing I’ve acquired all of those things in anticipation of this.

I realize you’re saying that overwhelmingly all of these things are unnecessary (maybe moreso with pans) but at least I felt like less of a weirdo since it looks like I followed your original path much to a tee as far as spending money on toys to “cool it up” or really in an effort to think that I would be uber-inflating my results :smile:


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137212 - 01/09/21 09:37 AM (3 years, 19 days ago)

glove box u say???? if so theres ur problem. your contamin shit out in the glove box and then putting it in an environment where it thrives.

its called a still air box for a reason. thats cuz it needs still air. when u have gloves n shit attached to it all it does is create a push/pull vaccum effect movin the air enuff to fuck ur shit up. and i wouldnt b the least bit shocked to see ur plates are trash after pouring in there even.

if your using glove box get rid of that shit👍👍


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Edited by jcm4620 (01/09/21 09:39 AM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137228 - 01/09/21 09:43 AM (3 years, 19 days ago)

pans are the only time u need any of the shit im using

but for growin cubes all u need is clean spawn, propperly prepped coir and verm sub,a light and a plastic tote thats it


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Edited by jcm4620 (01/09/21 09:44 AM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137250 - 01/09/21 09:50 AM (3 years, 19 days ago)

Quote:

Uzimyco said:
Cubes grow in warm places. Florida Brazil, Hawaii, Thailand. These are all zone 10 and higher.




Before mushroom cultivation, they also only grew outdoors (and not in the quantity we're able to reproduce on here). No need to mimic their natural environment, just give them what they want.

Put your previous experience on hold for just a second and learn how others do it on here, THEN use your expertise to adjust and adapt.

People won't be able to help you diagnose your strange facilities because no one here uses them.

Making things simpler, consistent, and more efficient are the main goals in cultivation.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137777 - 01/09/21 02:30 PM (3 years, 19 days ago)

Well wait then. Isn’t a glove box synonymous with still air box?  How would one work with anything inside of a closed box ?

Admittedly I’m the first to say that I realize the powerful limitations of any box set up. I hate it. As mentioned I’m patiently awaiting my filter so I can complete my flow hood, which I assume will be a major game changer. Hell it has to be.

I do think though that perhaps once reasonably perfected, agar is somewhat predictable but I would say there are far too many threads on the internet that talk about agar being simple and anyone can do it. I respectfully disagree.

I think agar is very unforgiving likely and requires outstanding levels of sterility. Why do I say that? Because I’ve done this with cakes many times, never had contamination issues and quite frankly wasn’t using the almost psychotic level of care I am now.

Yet every agar attempt has yielded some unforeseen problem. Again don’t misunderstand what I am saying.... I think agar is without question- the gold standard.  I just don’t think it’s quite the way some videos and threads portray. Again I’m saying this from some experience. Pf tek type stuff. Success success success and agar trouble trouble trouble. I know light years more than I did when I made the first cakes and those things blew up huge. Repeated many times.

Agar is challenging or at least so in my opinion without a flow hood. Since I’ve used hoods before in mericloning, meristem tissue extraction and fern spore plating im well aware of what the difference is. With a hood as long as it’s functioning properly and you follow the protocols- you’re basically good to go. With a box or otherwise and agar - it still appears to be a crapshoot.

My agar was pc at 15psi - 45 mins. I use high end lab individually wrapped sterile disposable innoc loops, syringes and uvc with an American lab dosimeter that ensures radioactive destruction of pathogens mechanically (there is no way to be tolerant to it. ). These are the same in research facilities and hospitals with CARF certification around the USA.  Yet still incur trouble?  I’m inclined to believe it’s a combination of agar and the box which no matter what you do at some point requires the entry or removal of something putting your work at risk whereas with a hood you have vastly more flexibility to work with things and ensure they remain in a sterile environment from a-z.  I’m not mycology pro but I’m no novice to science and sterility. 

Trust me. I’m VERY frustrated at myself about it. My experience should be helping to mitigate much of my failures here.  You’re looking at a person who did their first tissue culture plating likely well before most of the members of this forum were born. And believe me, there are numerous fern species growing in botanical gardens around the globe as the result of my spore work. Plants and fungi are not the same of course. Sterility often is.... and so I continue. I know it will get there. Hey, the bright side is , if it was super easy, everyone could do it. :thumbup:


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137800 - 01/09/21 02:42 PM (3 years, 19 days ago)

well then u should know the difference between a glove box and a still air box and understand how a vaccum created airflow would fuckin defeat the purpose of a still air box. hence the term STILL AIR🤷🏼maybe before tryin to impress everyone here with how vast ur scientific background is and writing a long ass post you should take a lil time and use the search engine on here in the upper right. it will tell you all u need to kno. you can basicly ask it a question. but that last long post  i didnt even bother to really read after the part about the glovebox not bein different i just kinda skimmed thru it as im certain thats causing ur problem and then ur placing it in the E Z bake contamination oven and making it even worse.

i bet u eliminate those 2 factors ur gnna be much better off👍


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Edited by jcm4620 (01/09/21 02:44 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137812 - 01/09/21 02:49 PM (3 years, 19 days ago)

heres the difference




the attached gloves are a huuuuge fuckin no no. they create a push/pull vaccum effect with every single slightest movement stirring up the internal air just enuff to shit all over your work.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137820 - 01/09/21 02:53 PM (3 years, 19 days ago)

These are some close ups of the growth in the original plates now. Maybe slightly better pics.

If anyone has thoughts that they are potentially good I’d want to do a transfer soon naturally. I’m not loving the new plates I did with dry spore. Different from the first set but I’m not clicking my heels.

Per my book above there may just be challenges with my box, or the spore prints or something else but I’d love to save something if possible. Please take a look and share your thoughts:




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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137836 - 01/09/21 03:00 PM (3 years, 19 days ago)

Check out the links that are in my signature. They're some of my favorite teks and I've ordered them in a way that is sort of from beginning to end as far as process goes. (Except for Bod's Teks) He has a big ass list of of teks he's made that work well for most people.

If you want to be successful, look at what everybody is doing or has done with great results. When you get a better understanding of what we're all doing and why, then you can make adaptions and other's will thank you for it. In the end, we're all after the same result. We want mushrooms and we want to see eachother succeed. This place and the people here can seem harsh at times but it really is for your own good.

Reddit, Discord and Youtube have some good things going on but I'd say about 95% of it is all unnessecary or misinformation. Stay away from those places. The search engine here should be your bible. If you have a question, someone has already asked it and it's documented there. Get familiar with it.

Those plates look good. That's what you're looking for.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137848 - 01/09/21 03:08 PM (3 years, 19 days ago)

Thank you. I appreciate the distinction between the two.

Not entirely sure how using gloved hands in the box with open airspace or the action of inserting ones hands wouldn’t risk carrying pathogens into the box, but I do understand what you’re describing.

I’m absolutely not trying to offend you in the least and certainly not trying to make you angry. Due to my confusion on the issue I did some quick searching around and my misunderstanding is evidently very common as I found piles of threads on this and other forums where the semantics are routinely held synonymous.  Wish I had known. The concept of a still air box appears significantly easier to construct.

I will say however that my glove box is fully stripped so as to eliminate air pressure from moving from outside to within.  Glove boxes are routinely used in hazmat situations where the individual is to be protected from the contents. It seems counterintuitive that such a design would allow for free flow in and out as the person would risk exposure but again the distinction is genuinely appreciated.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137852 - 01/09/21 03:10 PM (3 years, 19 days ago)

first off its not may be an issue with ur box. if u have gloves on it there IS an issue with ur box. a big one that will surely ruin all kinds of shit. so get a knife n chop that shit off completely.

as for ur plates that first one looks narly and i wouldnt ever touch anything close to an edge of a plate like that. now the second pic if u want u can take tfansfers from about the 2:00-5:00 positions and see what happens but dont place ao close to the edges. also u need to be using spore prints not syringes. streak in a zig zag pattern. plenty of good info in the search to belp ya along


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27137862 - 01/09/21 03:17 PM (3 years, 19 days ago)

Thank you Mario. I have looked at the links in your signature. Had done so before actually.

Not sure why everyone seems to think I’m looking to reinvent the wheel here. It’s simply not the case. I’m really not looking to do anything outside of what’s been described.

I did read your entire thread some time ago on the differences in mycelium vs a pathogenic mold. It’s very hard to tell or maybe my eyes are having a hard time making the distinctions. I know you notated the differences in the puffier fluffy look vs the coarser crunchy look. I am having a hard time
Telling the difference. I did post up some new pics of the spots in question with hopefully some better clarity. Hoping you all might be able to identify it?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137865 - 01/09/21 03:17 PM (3 years, 19 days ago)

i think u care more about playin with fancy lookin garbage than u do about getting good results


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137869 - 01/09/21 03:19 PM (3 years, 19 days ago)

ur not as smart as u think u are here man ive already explained it. its called a vaccum im really tryin to help u here man ditch that thing


and u may very well have it sealed perfect but guess what theres fuckin air inside of it. or do u suck all that out with another mr wizard unit while ur working??


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Edited by jcm4620 (01/09/21 03:31 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137878 - 01/09/21 03:26 PM (3 years, 19 days ago)

Quote:

Uzimyco said:
Thank you Mario. I have looked at the links in your signature. Had done so before actually.

Not sure why everyone seems to think I’m looking to reinvent the wheel here. It’s simply not the case. I’m really not looking to do anything outside of what’s been described.

I did read your entire thread some time ago on the differences in mycelium vs a pathogenic mold. It’s very hard to tell or maybe my eyes are having a hard time making the distinctions. I know you notated the differences in the puffier fluffy look vs the coarser crunchy look. I am having a hard time
Telling the difference.




It'll come with experience. You'll grow mold and eventually grow mycelium while you're still learning and then it will all make sense and you'll be able to tell the difference between the two when they're still barely visible colonies just beginning to form. If you have doubts, take a transfer and let it grow.

What JCM is explaining to you about the glove box being a bad idea is that when you make a proper glove box the air becomes perfectly still. Mold spores and other microscopic shit settles on the floor of the box or anything that it comes in contact with. When you put your hands into the gloves and move your arms, it creates a positive (or negative) air pressure which is enough force to pull anything off the ground and stir it around into the air allowing it to fall into your plates or anything else. With a still air box, the concept is similar except when everything settles on the floor, it stays there when you use slow movements. Because the box isn't sealed the pressure remains at 0.

I've seen all kinds of box ideas. Fans running with filters attached, bowls of water that supposedly draw the contaminants in because of static electricity... :smbfacepalm:

There's a reason nobody is using any of that stuff.


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Edited by Doctor Mario (01/09/21 03:29 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27137883 - 01/09/21 03:28 PM (3 years, 19 days ago)

you want a still air box not a glove box

bingo

all of which could have been found via the search in 30 seconds


Edited by jcm4620 (01/09/21 03:30 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137899 - 01/09/21 03:35 PM (3 years, 19 days ago)

I feel it's also worth mentioning that my sterile tech is complete shit. I'm staring at 28 contaminated plates as we speak. They never even got to see spores before they were laid to rest.
:futuramamario:

There's a reason JCM has the TC tag and not me. If you're gonna listen to anyone here, listen to the TC's.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137907 - 01/09/21 03:38 PM (3 years, 19 days ago)

Thanks jcm.  That is what I will do. Sadly they are the same plate at different locations but based on your descriptions and looking at the pics I see what you’re saying.

The first picture shows no threadlike growth around it so it likely is not very good.

2 o’clock and 5 o’clock on the other show clean thread like growth whereas say, 9 o’clock looks muddled and fuzzy.

That is amazingly helpful as I think I have an idea what I’m looking at based on what you’ve advised. Am I interpreting what you’ve said correctly?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137915 - 01/09/21 03:40 PM (3 years, 19 days ago)

Quote:

Uzimyco said:
2 o’clock and 5 o’clock




:thumbsup:


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27137927 - 01/09/21 03:44 PM (3 years, 19 days ago)

2 thru 5 take several and see what ya get. but plz dont do it in a gloved box ul really increase chances of failure.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137934 - 01/09/21 03:48 PM (3 years, 19 days ago)

u should be streakin plates in a zig zag pattern like so.  (just note this is pan myc)



or c10 has another very good streak method but i always just streak like my pic above.


heres a cube tissue clone



just cube myc


this plate was in the fridge for a long time which is why it looks like it does but if i was to want to take transfers off it id take from the leading edgeds of the sectored areas



then this is why u should stay away from syringes and why u should never go spores go grain




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Edited by jcm4620 (01/09/21 03:54 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27137958 - 01/09/21 03:57 PM (3 years, 19 days ago)

It was a quick and painless surgery. :grin:

Haha thank you!




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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27137964 - 01/09/21 03:58 PM (3 years, 19 days ago)

good deal make sure u got all the sleeve parts too. u dont want nothin that can move


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137981 - 01/09/21 04:04 PM (3 years, 19 days ago)

What food coloring brand is that? I dig that blue.I had a plate that I kept in the fridge and it also did something similar.
I think it froze and some of the myc died.


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Edited by Doctor Mario (01/09/21 04:07 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27137996 - 01/09/21 04:11 PM (3 years, 19 days ago)

it slowes down and almost eliminates rhizomorphic growth it seems thats why u can see the dull tome growth in the center n then when i took it out it came back to life ready to eat and just as vigorus as b4

i cant remember the brand it was a neon blue i dont even use anymore tho. i prefer just regular mea or lmea without dye


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Edited by jcm4620 (01/09/21 04:12 PM)


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27137998 - 01/09/21 04:13 PM (3 years, 19 days ago)

Not taking part in the charcoal craze?


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27138002 - 01/09/21 04:15 PM (3 years, 19 days ago)

Thank you for the pics jcm. Very helpful to see all of them.

Using your picture as a guide I marked up my photo. I did want to ask if what I have outlined would be the section taken in totality or would you suggest a smaller/larger portion?



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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27138015 - 01/09/21 04:24 PM (3 years, 19 days ago)

id take from inbetween also. id take 4 or 5 transfers total from that 2 theu 5 o clock area


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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
    #27138051 - 01/09/21 04:46 PM (3 years, 19 days ago)

Understood. Will do. Since your instructing up to 5 transfers within the 2-5 zone, is there a correct width you would recommend?

I have viewed things such as D3s use of punches (and yes as a result of course I currently posses them :blush: - you know... saw the toys so felt compelled to buy them - no fear. The buying gear frenzy is kind of out of the system).

In the case of that style he is suggesting what are naturally circular sizable chunks. Since your instruction is up to 5 in the area I would take slimmer sections with a scalpel. Would you recommend a minimum width in section? Perhaps 2mm? Maybe more?  Apologies for the very overly detailed question, but your experience and time isn’t necessarily readily available so I’d love to take any of my potential assumptions out of the equation and get very detailed info from you while I have your ear.

I’m genuinely appreciative of your time :thumbup:


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27138128 - 01/09/21 05:41 PM (3 years, 19 days ago)

Some people take transfers the size of a grain of rice. Use your best judgement.


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27138293 - 01/09/21 07:12 PM (3 years, 19 days ago)

Quote:

Doctor Mario said:
Some people take transfers the size of a grain of rice. Use your best judgement.





Many thanks Mario. So just like a sliver or somewhat larger so as to get the multiple transfers from that area. I’ll have to wait until tomorrow as I realized now that I was out of ready plates. I’ll
make agar in the am and as soon as it’s poured/cooled I’ll give it a go.

Will update with hopefully positive result when there is some result to report :thumbup:


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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27148566 - 01/14/21 08:08 PM (3 years, 14 days ago)

Jcm and Mario-

Any help would be appreciated. These are the 2 transfers I was able to get.  They’ve grown out a bit. Really not sure what’s up here. 2 of the pics are the same transfer with different lighting.

Hoping you can decipher what’s going on here?




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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
    #27148863 - 01/14/21 11:49 PM (3 years, 13 days ago)



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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
    #27149050 - 01/15/21 03:51 AM (3 years, 13 days ago)

when I saw your original post I figured it was the water which was the problem..  since it was bacteria and not mold,, the first agar dish I grew. was purchased pre done..so it was clean..  first try  I grew about three to six different molds and a bacteria clearly the reaction on the agar to the bacteria was so much different than the mold.. and the molds were so much different in color and growth pattern I learned a LOT form that single experimental agar dish... and I was able to transfer clean myc off as well.. I let it grow (mold) and watched the molds compete for king of the agar hill... you sound like a scientific guy so I think your gonna figure it out..and yes some research tec is out dated and some people call still air boxes (glove boxes) but they are different in design positive pressure systems and clean and still air...however its achieved is what you want.. I don't think agar is hard to achieve for the average IQ (or I'd be out)  I've watched a lot of vids and learned a lot from Utube etc..I have a UV type light for my fish tank and yes it kills the fuck out of algae but it can't be in the same tank as the fish or it will nuke em too..I don't see it being needed in this arena as its a added cost that is a waste of $$ /time.. cloning wild mush and growing mush from the super market is amazing and fun..I don't mind the SAB but I do like a nice Plexiglass one (fish tank) its perfect,  over the clear tote I started with way easier to see.. I'm still a cherry but I've had a lot of first time goes on stuff that is difficult for people to do and I credit this place reading stuff and utube..


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