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Doctor Mario
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Registered: 08/07/20
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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
#27137981 - 01/09/21 04:04 PM (3 years, 19 days ago) |
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What food coloring brand is that? I dig that blue.I had a plate that I kept in the fridge and it also did something similar. I think it froze and some of the myc died.
Edited by Doctor Mario (01/09/21 04:07 PM)
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jcm4620
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
#27137996 - 01/09/21 04:11 PM (3 years, 19 days ago) |
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it slowes down and almost eliminates rhizomorphic growth it seems thats why u can see the dull tome growth in the center n then when i took it out it came back to life ready to eat and just as vigorus as b4
i cant remember the brand it was a neon blue i dont even use anymore tho. i prefer just regular mea or lmea without dye
Edited by jcm4620 (01/09/21 04:12 PM)
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Doctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
#27137998 - 01/09/21 04:13 PM (3 years, 19 days ago) |
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Not taking part in the charcoal craze?
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Uzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
#27138002 - 01/09/21 04:15 PM (3 years, 19 days ago) |
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Thank you for the pics jcm. Very helpful to see all of them.
Using your picture as a guide I marked up my photo. I did want to ask if what I have outlined would be the section taken in totality or would you suggest a smaller/larger portion?
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jcm4620
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
#27138015 - 01/09/21 04:24 PM (3 years, 19 days ago) |
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id take from inbetween also. id take 4 or 5 transfers total from that 2 theu 5 o clock area
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Uzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: jcm4620]
#27138051 - 01/09/21 04:46 PM (3 years, 19 days ago) |
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Understood. Will do. Since your instructing up to 5 transfers within the 2-5 zone, is there a correct width you would recommend?
I have viewed things such as D3s use of punches (and yes as a result of course I currently posses them - you know... saw the toys so felt compelled to buy them - no fear. The buying gear frenzy is kind of out of the system).
In the case of that style he is suggesting what are naturally circular sizable chunks. Since your instruction is up to 5 in the area I would take slimmer sections with a scalpel. Would you recommend a minimum width in section? Perhaps 2mm? Maybe more? Apologies for the very overly detailed question, but your experience and time isnβt necessarily readily available so Iβd love to take any of my potential assumptions out of the equation and get very detailed info from you while I have your ear.
Iβm genuinely appreciative of your time
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Doctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
#27138128 - 01/09/21 05:41 PM (3 years, 19 days ago) |
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Some people take transfers the size of a grain of rice. Use your best judgement.
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Uzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
#27138293 - 01/09/21 07:12 PM (3 years, 19 days ago) |
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Quote:
Doctor Mario said: Some people take transfers the size of a grain of rice. Use your best judgement.
Many thanks Mario. So just like a sliver or somewhat larger so as to get the multiple transfers from that area. Iβll have to wait until tomorrow as I realized now that I was out of ready plates. Iβll make agar in the am and as soon as itβs poured/cooled Iβll give it a go.
Will update with hopefully positive result when there is some result to report
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Uzimyco
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
#27148566 - 01/14/21 08:08 PM (3 years, 14 days ago) |
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Jcm and Mario-
Any help would be appreciated. These are the 2 transfers I was able to get. Theyβve grown out a bit. Really not sure whatβs up here. 2 of the pics are the same transfer with different lighting.
Hoping you can decipher whatβs going on here?
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Doctor Mario
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Uzimyco]
#27148863 - 01/14/21 11:49 PM (3 years, 13 days ago) |
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Blackrainbow2
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Re: Anyone have link or pics of early stage agar inoculation? [Re: Doctor Mario]
#27149050 - 01/15/21 03:51 AM (3 years, 13 days ago) |
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when I saw your original post I figured it was the water which was the problem.. since it was bacteria and not mold,, the first agar dish I grew. was purchased pre done..so it was clean.. first try I grew about three to six different molds and a bacteria clearly the reaction on the agar to the bacteria was so much different than the mold.. and the molds were so much different in color and growth pattern I learned a LOT form that single experimental agar dish... and I was able to transfer clean myc off as well.. I let it grow (mold) and watched the molds compete for king of the agar hill... you sound like a scientific guy so I think your gonna figure it out..and yes some research tec is out dated and some people call still air boxes (glove boxes) but they are different in design positive pressure systems and clean and still air...however its achieved is what you want.. I don't think agar is hard to achieve for the average IQ (or I'd be out) I've watched a lot of vids and learned a lot from Utube etc..I have a UV type light for my fish tank and yes it kills the fuck out of algae but it can't be in the same tank as the fish or it will nuke em too..I don't see it being needed in this arena as its a added cost that is a waste of $$ /time.. cloning wild mush and growing mush from the super market is amazing and fun..I don't mind the SAB but I do like a nice Plexiglass one (fish tank) its perfect, over the clear tote I started with way easier to see.. I'm still a cherry but I've had a lot of first time goes on stuff that is difficult for people to do and I credit this place reading stuff and utube..
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