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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27127918 - 01/05/21 10:36 AM (3 years, 1 month ago)

Alright I’m in the game

I streaked plates of Melmac, TWC (beautiful print by the way! You rock Ted!), and a Pan cyan “Estero” from asura from a while ago. I’ll update my op when I get near a computer but I’m excited to grow along with y’all!


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Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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OfflineGypsyBastard
Misanthrope
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Registered: 11/30/20
Posts: 846
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Leon Forest]
    #27127967 - 01/05/21 11:07 AM (3 years, 1 month ago)

Quote:

Indulgence said:

Box is ready






Holy shit Indulgence, I just saw this photo. Is that an induction coil set up in there. Ha I thought I was the only electro nerd using one of those.


Edited by GypsyBastard (01/05/21 11:11 AM)


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
    #27128088 - 01/05/21 12:06 PM (3 years, 1 month ago)

Quote:

Ambrose said:
Count me in please!
Planning to grow some P. Mexicana Jalisco and Chicon Nindo, that is if they ever get out of USPS "in transit, arriving late" purgatory.





Prints finally arrived! Might have been duped by the seller but we shall see :shrug:







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InvisibleDoctor Mario
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Registered: 08/07/20
Posts: 1,894
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
    #27128214 - 01/05/21 12:58 PM (3 years, 1 month ago)

Why swab a print? Nocc loop for the win.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27128242 - 01/05/21 01:12 PM (3 years, 1 month ago)

Quote:

Doctor Mario said:
Why swab a print? Nocc loop for the win.




I actually played around on my lab session before last for a funsie comparison.
Swabs definitely did a better job at picking up spores; just a touch needed instead of lateral motion with a loop. I may be wrong but in my mind it seems like less surface contact equates to less chance or contams being transfered.
The flip side is the spores like to stick to the swab making it a bit difficult to see if any were deposited.

Worth noting: This was done with a DIY nicrhome loop and self sterilized q-tips as swabs. I would have loved a longer handle on the swabs and would probably make me like them more. For now, I'm sticking with my loop.


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27128248 - 01/05/21 01:14 PM (3 years, 1 month ago)

Well those swabs probably come pre-sterilized which makes it a simple grab, wipe, swipe.
So I can see why but nocc loop is what you ought to use.


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27128271 - 01/05/21 01:26 PM (3 years, 1 month ago)

Quote:

Doctor Mario said:
Why swab a print? Nocc loop for the win.



I prefer how they feel and they work fine. The nocc loop I made felt like shite and I'm too lazy to try making a better one.

Plus I'm a clumsy mofo so anything that removes the need for a torch is a good thing.


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OfflineOne of Us
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead] * 2
    #27128280 - 01/05/21 01:28 PM (3 years, 1 month ago)

Quote:

mushhead said:
Well those swabs probably come pre-sterilized which makes it a simple grab, wipe, swipe.
So I can see why but nocc loop is what you ought to use.





Ought, shmought. You'll never take me alive! Sterile swabs for life!


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: One of Us]
    #27128299 - 01/05/21 01:36 PM (3 years, 1 month ago)

Quote:

One of Us said:
Quote:

mushhead said:
Well those swabs probably come pre-sterilized which makes it a simple grab, wipe, swipe.
So I can see why but nocc loop is what you ought to use.






Ought, shmought. You'll never take me alive! Sterile swabs for life!




:snoopyes:


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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: One of Us]
    #27128302 - 01/05/21 01:37 PM (3 years, 1 month ago)

Quote:

One of Us said:
Quote:

mushhead said:
Well those swabs probably come pre-sterilized which makes it a simple grab, wipe, swipe.
So I can see why but nocc loop is what you ought to use.





Ought, shmought. You'll never take me alive! Sterile swabs for life!



That sounds like a pretty good testamonial.
What swab TEK or technique do you use?


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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
    #27128309 - 01/05/21 01:39 PM (3 years, 1 month ago)

Quote:

Ambrose said:
Quote:

One of Us said:
Quote:

mushhead said:
Well those swabs probably come pre-sterilized which makes it a simple grab, wipe, swipe.
So I can see why but nocc loop is what you ought to use.






Ought, shmought. You'll never take me alive! Sterile swabs for life!




:snoopyes:



:teabird: nocc loop tribe for life.
Though I have not once used a swab to spread spore onto a plate, I wonder how it would perform for me.


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,728
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27128322 - 01/05/21 01:44 PM (3 years, 1 month ago)

Would you swabbers recommend I sterilize some qtips, squirt it with some of my MSS and go to agar? With MSS I'm getting unpredictable results at best using MSS -> loop -> agar


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I :heart: 5318008


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OfflineBallzagna
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Inthepit]
    #27128348 - 01/05/21 01:51 PM (3 years, 1 month ago)

Quote:

Inthepit said:
Quote:

One of Us said:

Ought, shmought. You'll never take me alive! Sterile swabs for life!



That sounds like a pretty good testamonial.
What swab TEK or technique do you use?




Sterile swab gang reporting in

I think Munch has a video on it in his collection but just a small roll of the swab on the print gets a good amount of spores and then swipe it on the plate, I usually streak 2-4 plates per swab and 2 swabs come in a pack but they're pretty cheap so sometimes I just end up tossing one or letting the cat play with it. Something about cats and swabs...

https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261 Volume 3b is the swab vid


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No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
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:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs




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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27128355 - 01/05/21 01:54 PM (3 years, 1 month ago)

Swab Gang 4 life!

As for MSS to a swab, ive done it numerous times... you'll still get a little bit of bacteria but I much prefer the swabs because they'll soak up all the excess water for the syringe... just my 2€...


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OfflineThe Dalcassian
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
    #27128361 - 01/05/21 01:56 PM (3 years, 1 month ago)

Just to be awkward, I use the scalpel tip to inoculate plates. Normally do about 5 points per plate

Tried swabbing for LAGM tho and it's worked but so does the scalpel


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Here Lies This Individual





Edited by The Dalcassian (01/05/21 01:57 PM)


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27128373 - 01/05/21 02:02 PM (3 years, 1 month ago)

Quote:

bigfootscreepyuncl said:
Would you swabbers recommend I sterilize some qtips, squirt it with some of my MSS and go to agar? With MSS I'm getting unpredictable results at best using MSS -> loop -> agar





Sounds like your using a syringe? Take my advice with a grain of salt as I'm still a noob. I had a syringe that showed nothing but bacteria on agar and I found a brf puck worked pretty good to get it to germinate when I was having trouble.

Went from 0/20 germinating on agar(using both a loop and swabs) to 3/6 germinating on a brf puck.
Here's Josex's tek:
https://www.shroomery.org/forums/showflat.php/Number/24806569/fpart/all/vc/1


Edited by Ambrose (01/05/21 02:03 PM)


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InvisibleDoctor Mario
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: The Dalcassian]
    #27128377 - 01/05/21 02:05 PM (3 years, 1 month ago)

Well, after all these pro-swabbers I'm actually excited about the swabs that I'm using for this LAGM. Still waiting to verify that the plates I poured are contam free. I usually wait around 10 days but I'm getting ansy.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
    #27128390 - 01/05/21 02:09 PM (3 years, 1 month ago)

:brainondrugs: Team learning and adapting for life!

If putting mss on agar, I usually go with a swab. Helps with the excess moisture. Loops is a bit easier to use imo. Less wrapping and such to remove and and less waste. Pros and cons; strikes and gutters.....


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OfflineTattersail
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27128408 - 01/05/21 02:16 PM (3 years, 1 month ago)

I tried using a homemade loop once and that was a terribly fiddly affair. I sterilised some swabs for this grow but ended up just using the scalpel to gently scrape the print, then kinda stab it into the agar in a few places. It's always worked just fine before.


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Invisibleallthespores


Registered: 12/14/20
Posts: 42
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: allthespores]
    #27128428 - 01/05/21 02:26 PM (3 years, 1 month ago)

3 plates show good growth. no contams yet.  :awesome:
should i take some mycel from all of them, if i want to do 4 jars of wbs  or is there something better i should do?
I have no experience working with plates, so im clueless what to do with more then one. :tongue2:
Thinking about storing one, transfer one and the third goes to grain.



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LAGM2021 Grow log


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