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OfflineCelestialexplorer1
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Registered: 03/25/20
Posts: 1,803
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Re: Some notes about LCs [Re: A.k.a]
    #27102036 - 12/22/20 05:47 AM (3 years, 1 month ago)

Try leaving it alone until the myc recovers and don’t shake till it’s started fluffing up and attaching to itself which takes one day to four days.


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InvisibleleroM
Registered: 11/13/17
Posts: 83
Re: Some notes about LCs [Re: Celestialexplorer1] * 1
    #27102104 - 12/22/20 07:18 AM (3 years, 1 month ago)



Stamets, in Growing Gourmet and Medicinal Mushrooms, pg 137, recommends stirring at 100-200 rpm.


Edited by leroM (12/22/20 08:06 AM)


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Invisiblemicelio
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Registered: 04/22/14
Posts: 1,547
Re: Some notes about LCs [Re: leroM]
    #27102535 - 12/22/20 01:14 PM (3 years, 1 month ago)

Lerom

That's good stuff.. Thanks. (The mycelium may suffocate and rise to the surface of the liquid in search of air..)


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InvisibleleroM
Registered: 11/13/17
Posts: 83
Re: Some notes about LCs [Re: micelio]
    #27102564 - 12/22/20 01:35 PM (3 years, 1 month ago)

My pleasure, man.

Another thing i’ve picked up, but can’t remember who to attribute: recovery time is due in part to cells building enzymes to metabolize the specific sugars in the LC...to aid this process, it helps to include in your plate agar small amounts of the specific sugars that will be used in the LC broth. Extending this logic, it might also help in preparing mycellia for nutritional components of spawn/substrate.


Edited by leroM (12/22/20 01:58 PM)


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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
Re: Some notes about LCs [Re: leroM]
    #27102788 - 12/22/20 04:59 PM (3 years, 1 month ago)

Quote:

leroM said:


Stamets, in Growing Gourmet and Medicinal Mushrooms, pg 137, recommends stirring at 100-200 rpm.



*Adds book to growing list of books on mycology.*
:takingnotes:


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InvisibleleroM
Registered: 11/13/17
Posts: 83
Re: Some notes about LCs [Re: mushhead]
    #27103572 - 12/23/20 05:51 AM (3 years, 1 month ago)

Been doin a bench trial that shows a few things that may be of interest with respect to this thread, at https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27088562&page=0&vc=1#27088562


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Offlinesonoramo
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Re: Some notes about LCs [Re: leroM]
    #27104146 - 12/23/20 12:34 PM (3 years, 1 month ago)

Quote:

leroM said:
Been doin a bench trial that shows a few things that may be of interest with respect to this thread, at https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27088562&page=0&vc=1#27088562



Quote:

leroM said:
Been doin a bench trial that shows a few things that may be of interest with respect to this thread, at https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27088562&page=0&vc=1#27088562




Nice!

I've never used the Shroomery's "journal" feature before. What's the best way to follow so I see posts as they come up? On this thread, Community >> Threads shows threads I follow with new entries highlighted, but that doesn't seem possible with a journal.


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Invisiblemushhead
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Re: Some notes about LCs [Re: sonoramo]
    #27104162 - 12/23/20 12:43 PM (3 years, 1 month ago)

Quote:

sonoramo said:
Quote:

leroM said:
Been doin a bench trial that shows a few things that may be of interest with respect to this thread, at https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27088562&page=0&vc=1#27088562



Quote:

leroM said:
Been doin a bench trial that shows a few things that may be of interest with respect to this thread, at https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27088562&page=0&vc=1#27088562




Nice!

I've never used the Shroomery's "journal" feature before. What's the best way to follow so I see posts as they come up? On this thread, Community >> Threads shows threads I follow with new entries highlighted, but that doesn't seem possible with a journal.



idk, it seems like if you want to keep up with a journal of an individual you'd have to just keep checking out their journal *shrug*


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OfflineBrownBear
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Re: Some notes about LCs [Re: mushhead]
    #27104267 - 12/23/20 01:48 PM (3 years, 1 month ago)

It is possible as long as you have permission from the journal op and they allow you to post in it. Otherwise save the link to your favorites.


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Invisiblemushhead
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Re: Some notes about LCs [Re: BrownBear]
    #27114948 - 12/29/20 08:59 PM (3 years, 1 month ago)


PESA - 30cc syringe.
Being tested on agar rn.


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InvisiblePastywhyteMDiscord
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Re: Some notes about LCs [Re: mushhead] * 1
    #27115222 - 12/29/20 11:37 PM (3 years, 1 month ago)

Is that an EzLC? Looks clear to me.


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OfflineA.k.aM
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Re: Some notes about LCs [Re: Pastywhyte]
    #27115369 - 12/30/20 03:01 AM (3 years, 1 month ago)

lol I was confused at first but I think I recognize that rhizo. Pretty sure it’s a plate filled with water with the myc scraped off. I think.


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Invisiblemushhead
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Registered: 08/22/14
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Re: Some notes about LCs [Re: A.k.a]
    #27116137 - 12/30/20 11:56 AM (3 years, 1 month ago)

So I actually just filled one of my no pour agar jars with some low nute LC.
I let it grow out and colonize the 30ml of LC I had in there.
Stirred it up good, even was able to release the myc from the agar plate itself, grabbed a 12g sterilized luer lock blunt tip to fill the syringe up with LC.
-> -> -> -> ->
All this was done in an SAB. Syringe/Needle came pre-sterilized.
I have you to thank pasty for the idea.


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Edited by mushhead (12/30/20 12:08 PM)


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: mushhead]
    #27125680 - 01/04/21 09:35 AM (3 years, 1 month ago)

If you guys are inoculating a 5lb grain bag how much LC would you use?


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineCamera93
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Re: Some notes about LCs [Re: PitcherCrab]
    #27125685 - 01/04/21 09:39 AM (3 years, 1 month ago)

I have used 20ml
I nocced 3 bags with a 60ml LC syringe

I want to chime into these questions more often, I should include a disclaimer that I have only gone this route once. I tried again with like 5-10ml and it was much slower. but again not sure if maybe my grain prep was off on the last attempt slowing me down.

I plan to return to bags and that 20ml will be my starting point again


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Close your eyes, and do the best that you can



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OfflinePitcherCrab
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Re: Some notes about LCs [Re: Camera93]
    #27125687 - 01/04/21 09:42 AM (3 years, 1 month ago)

Word. Yea, I was thinking between 10-20cc. I’ll use 20 when I do my oat bags today.


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisiblePastywhyteMDiscord
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Re: Some notes about LCs [Re: PitcherCrab]
    #27125726 - 01/04/21 10:05 AM (3 years, 1 month ago)

I used to use 20 but sometimes I’d spy pooling, I prefer 15 now.


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: Pastywhyte]
    #27125738 - 01/04/21 10:16 AM (3 years, 1 month ago)

Quote:

Pastywhyte said:
I used to use 20 but sometimes I’d spy pooling, I prefer 15 now.




Excellent, thank you!


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineFailboat
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Re: Some notes about LCs [Re: PitcherCrab]
    #27126965 - 01/04/21 07:44 PM (3 years, 1 month ago)


Did some ~4% ME. All 3 Melmac from the same plate. Definitely seems to be an improvement over past LC attempts.


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OfflineA.k.aM
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Re: Some notes about LCs [Re: Failboat]
    #27127080 - 01/04/21 08:47 PM (3 years, 1 month ago)

Was your past lc higher or lower %?


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