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anon0101
aeon



Registered: 11/28/20
Posts: 60
Loc: Al’Taieu
Last seen: 2 years, 8 months
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baby mycelium or contam
#27126241 - 01/04/21 01:59 PM (3 years, 24 days ago) |
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i’m curious about a few things i see in my pd’s. this being my first attempt with cultivation i suck bad so i had 3 agar plates that didn’t even cover the dish, i used these to determine how sterile my procedure is. they have some things stuck in the matrix that i believe might be just unmixed agar or something.
so i have brazil, ecuador, and pf redspore now since i threw out my dancing tiger pd’s because of orangeish bacteria. the pictures in question are 1. PF REDSPORE Is this mycelium trying to grow out or contam? Is the pd contam on the bottom? 
2. ECUADOR Salvageable? I believe this is contam just not sure how bad.

3. BRAZIL Also believe these to be contam just wondering if anything is salvageable.

It is hard to see with the condensation. these are all incubating upside down right now (75-79°F)because i was worried the condensation was gona create more contam then i possibly introduced. i will try to use a warm cup on top of the pd’s next week then leave them alone until i see real growth.
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: baby mycelium or contam [Re: anon0101]
#27126255 - 01/04/21 02:05 PM (3 years, 24 days ago) |
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It all looks bacterial to me. Not sure what's going on in the last picture. Can't really see what's going on with your redspore.
Even though your plates are bacterial you can still let the myc grow out larger then take some VERY small xfers to new dishes. That is the main purpose of using agar in the first place.
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: baby mycelium or contam [Re: tiptrippy]
#27126270 - 01/04/21 02:11 PM (3 years, 24 days ago) |
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Its all bacteria. When it's that much I doubt any myc will be able to out grow it. It looks like the syringe has the bacteria too, but can't be sure since you said your technique isn't the best
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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anon0101
aeon



Registered: 11/28/20
Posts: 60
Loc: Al’Taieu
Last seen: 2 years, 8 months
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Re: baby mycelium or contam [Re: anon0101]
#27126347 - 01/04/21 02:50 PM (3 years, 24 days ago) |
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Thanks for the quick responses, looks like i have a lot of practice to do. i will keep up the plates out of curiousity and restart next week. in the mean time i will set up pf tek and look for other uses of my spore syringes.
does anyone know of some pd that have reliably been sterile? or if i could sterilize them myself i will. these i got from amazon come in 20, very flimsy and stick together.
i hope all of my syringes are not contaminated and i just suck at being sterile.
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