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Artfulmushroom
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Registered: 12/13/20
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Last seen: 2 years, 9 months
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Agar Questions
#27125193 - 01/04/21 04:03 AM (3 years, 24 days ago) |
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Hey All,
Hope everyone is doing well!
I'm currently waiting to harvest my second flush on 3 shoeboxes and I wanted to clone a couple of mushrooms to transfer etc.
I have a pressure cooker and all the tools required to make agar and ingredients to do a PDA. I also bought glass petri dishes cause of the environment you know... but starting to think it's gonna be a pain to sterilize them all the time and I only have 10 dishes.
Down to the Q's:
When's best to clone a mushroom and what tek do you prefer? Whats the best way to do a PDA Agar what tek do you prefer? Seen a lot of people put food colouring in the Agar, yes or no?
*edit* If seeing the tubs would help, lemme know
Edited by Artfulmushroom (01/04/21 04:18 AM)
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jcm4620
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Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
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i prefer just a mea or lmea agar tho. why do you wish to go pda ? is there a reason?? just curious. also the best and easiest way for either in my opinion and what i use is a pre mix all u gtta do is measure out add water pc and pour. very very easy and i can tell you where i buy mine from if u wanna pm me as naming vendors in the cult theead is against the rules.
you can clone any fruit at any time. however i prefer a fully mature fruit with desired traits by taking a tissue sample to agar. some ppl will clone by putting pins directly to agar. its all up to you but i prefer to clone for specific traits/phenotypes
Edited by jcm4620 (01/04/21 04:48 AM)
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RenegadeMycologist
On the case



Registered: 12/05/20
Posts: 3,817
Loc: Serbia
Last seen: 8 days, 11 hours
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Re: Agar Questions [Re: jcm4620]
#27125243 - 01/04/21 04:48 AM (3 years, 24 days ago) |
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How to know what agar to use ? I ve seen many different recipes/types
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l e a r n i n g t h i n g s
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RenegadeMycologist
On the case



Registered: 12/05/20
Posts: 3,817
Loc: Serbia
Last seen: 8 days, 11 hours
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Why not pda
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l e a r n i n g t h i n g s
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jcm4620
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Registered: 05/26/19
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Last seen: 1 year, 11 months
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i just prefer mea or lmea less of a hassle less ingriedients and better results in my opinion.
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Artfulmushroom
Stranger

Registered: 12/13/20
Posts: 31
Last seen: 2 years, 9 months
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Re: Agar Questions [Re: jcm4620]
#27125304 - 01/04/21 05:38 AM (3 years, 24 days ago) |
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Quote:
jcm4620 said: i prefer just a mea or lmea agar tho. why do you wish to go pda ? is there a reason?? just curious. also the best and easiest way for either in my opinion and what i use is a pre mix all u gtta do is measure out add water pc and pour. very very easy and i can tell you where i buy mine from if u wanna pm me as naming vendors in the cult theead is against the rules.
you can clone any fruit at any time. however i prefer a fully mature fruit with desired traits by taking a tissue sample to agar. some ppl will clone by putting pins directly to agar. its all up to you but i prefer to clone for specific traits/phenotypes
I have the ingredients already for PDA and I was looking at a TEK a while back which suggested PDA for cubes.
In regards to the cloning pre or post spore drop? Or does it not matter?
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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I would clone predrop before the veils open. You can take a small sample from just below the cap. Cut off cap and open the stem and scrap a small amount of tissue from the inside of the stem and put it to agar.
The glass plates are fine, but you will want to pour them all at once and save some for transfers. Pour'd plates can be fine to leave out for a long time if wrapped correctly.
ALso if you have PDA use it, but I agree that LME is easier. I order Agar-Agar and LME and just scale it and add water.
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