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OfflineTigerStrike
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Registered: 12/22/20
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Agaricus Bispours on Agar
    #27125099 - 01/04/21 01:36 AM (3 years, 1 month ago)

been on agar for 4 days no germination presence of spores detected what do you guys think? 


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Invisiblealaskappalachian
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Registered: 10/22/19
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Re: Agaricus Bispours on Agar [Re: TigerStrike] * 1
    #27125104 - 01/04/21 01:43 AM (3 years, 1 month ago)

With my a. bisporus I had to go gill tissue to agar to get good myc to snag.  They are fucking loaded with contaminants so you want to do several plates initially.  You'll get better (faster,  which is important) germ with gill tissue,  and you'll need speed on your side to outrun contaminants.  Good luck bud.  As I recall you're trying this for your LAGM?


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Agaricus Bispours on Agar [Re: TigerStrike]
    #27125105 - 01/04/21 01:44 AM (3 years, 1 month ago)

be patient:super:silver lining you dont have contams yet


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OfflineTigerStrike
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Registered: 12/22/20
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Re: Agaricus Bispours on Agar [Re: cronicr]
    #27125119 - 01/04/21 02:10 AM (3 years, 1 month ago)

I am participating in LAGM this is intended to be a spore transfer not using ideal methods for this type of agar i would have just taken an inner tissue sample otherwise. Thanks for the encouragment.

These were scraped from the bottom of Styrofoam container of a sliced portable. Yes I know very dirty we'll see if I can snag some mycellium. Thanks for the replies.


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