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Offline76Salvia
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Does it makes any sense? (Cutting agar contams)
    #27124235 - 01/03/21 03:57 PM (3 years, 25 days ago)

Hello friends~

I ran out of agar plates (and agar, in general), and I have a couple of plates with some contamination on one side (already with high % mycelium and so on).

Does it make any sense to cut the entire contaminated area of ​​agar (+ a little bit more)?

At least to slow it down until I have more for cleaning, or to use the healthy mycelium areas to inoculate smth.

Will try, I dont really have anything to lose hahahaha

Lets see what happend~

:nyan:


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OfflineAlsetAlokin
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Re: Does it makes any sense? (Cutting agar contams) [Re: 76Salvia]
    #27124283 - 01/03/21 04:29 PM (3 years, 25 days ago)

Sounds like a bad idea, and I'd advise against it if it's your last shot.

But experimenting is always fun, and if you plan on cutting the bad stuff out and storing those plates until you can make transfers in the future I don't see why not. (Granted they haven't sporulated)

If I were in your shoes I would put the good myc to grain and then sit on that grain master until ready to expand or top fruit and get something worth cloning.

What's holding you off on making more plates and getting more supplies?


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Offline76Salvia
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Re: Does it makes any sense? (Cutting agar contams) [Re: AlsetAlokin]
    #27124442 - 01/03/21 05:58 PM (3 years, 25 days ago)

Quote:

AlsetAlokin said:
Sounds like a bad idea, and I'd advise against it if it's your last shot.

But experimenting is always fun, and if you plan on cutting the bad stuff out and storing those plates until you can make transfers in the future I don't see why not. (Granted they haven't sporulated)

If I were in your shoes I would put the good myc to grain and then sit on that grain master until ready to expand or top fruit and get something worth cloning.

What's holding you off on making more plates and getting more supplies?




About holding me of... just time, tbh, in a few days I will prepare more agar and do some transfers.

But I was thinking about it before, in the same way that a plate is cleaned by taking pieces of clean agar (with myc), I don't see that removing the contaminated part from a plate is such a bad idea. At least as a temporary thing, idk.

Experimenting is always fun, soooo... let's see what happens hahahaha

:pondering:

I mean, its just a first or second transfer from spore prints, so I have more spores, just in case

~

I never read about this procedure, thats why I ask, just curiosity about it. I guess it's not a very safe procedure for the culture, I don't know.


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OfflineShakedown Street
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Registered: 09/05/20
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Re: Does it makes any sense? (Cutting agar contams) [Re: 76Salvia]
    #27124678 - 01/03/21 08:19 PM (3 years, 25 days ago)

I lost many agar cultures in petri dishes to contamination last year.  A brownish mold.    I tried cutting out all the brownish spots and flooding the holes in the agar w/ hydrogen peroxide.    Don't recall ever seeing any healthy mycelium again in any of those agar dishes with holes.

I would suggest instead of trying to cut out the contaminated agar you instead sterilize a scalpel and try to cut out the tiniest piece of white mycelium from a contaminated agar culture-- like 1 mm by 1 mm.  Transfer it onto a virgin agar plate.  Flush the rest of the contaminated agar culture down the toilet.    Wash your hands w/ soap & water.


Edited by Shakedown Street (01/03/21 08:20 PM)


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OfflineBlackrainbow2
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Re: Does it makes any sense? (Cutting agar contams) [Re: Shakedown Street]
    #27124937 - 01/03/21 10:52 PM (3 years, 24 days ago)

I did it the other night I had a white oyster spore print I had made and it was a little wet on the tinfoil. I scraped the spores onto agar into two spots on the plate the first spot blew up green mold right away and the second spot was white and clean I just cut it out (green spot)  so far so good.. kinda exciting I had nothing to loose..but I can see if the mold was advanced your likely to just spread it around on the plate..


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