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InvisibleScienceDraven
Explorer
Registered: 01/03/21
Posts: 3
Pin in jar, or out of jar. Which is better?
    #27123950 - 01/03/21 01:26 PM (3 years, 25 days ago)

Hi All. This is my first post. I'm excited to be a part of this community. If I am off track, gentle guidance would be greatly appreciated. I will try to keep this succinct. I am a man of science and like to know the "whys" of what I do, and am trying different techniques, side by side for my first exploration in to this hobby.

My end goal is to create an automated and controlled environment that reliably produces. Therefore, I would like to avoid the fanning and misting by creating a controlled environment using sensors, humidifiers, fans, timers, etc. I have done this in small scale and will scale up later.

I am using variations on the BRF / PF Tek. Most teks suggest birthing in to an FC, dunking, casing, fanning, misting, etc. However, PF Tek for Simple Minds suggests allowing the pinning to occur in the closed jar and then birthing once mushroom development starts to occur.

After 1 week in my FC, my "straight to FC" cakes showed obvious, healthy activity, which slowed as the cakes started to dry out more. Remember, I am controlling the environment and avoiding misting and fanning. Clearly, there is some issue with the cakes getting too dry which halted some of the mycelial activity. However, the jars I left closed for 7 days, didn't appear to be doing much. So, I birthed them. To my surprise, there was a lot of active mycelial (fuzzy) activity that couldn't be seen in the jar. It actually looked quite healthy.

Considering that I want to avoid the misting and simply maintaining a 95% humidity chamber may not work well. Does it make more sense to allow the pinning to happen in the jar, where moisture is contained, and then birth to FC? Or, is it better to birth to FC and mist as necessary if they start to dry?

Either way, I will test and update this thread in the future.


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OfflineInnerWisdom
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Registered: 08/09/19
Posts: 1,936
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Last seen: 4 days, 9 hours
Re: Pin in jar, or out of jar. Which is better? [Re: ScienceDraven]
    #27123961 - 01/03/21 01:33 PM (3 years, 25 days ago)

I don't see how pinning would happen in the jar unless there was sufficient FAE, or the mycelium was really desperate to push out a mushroom. That happened to me in a jar of agar once.

You should know that to get a good set of pins the conditions need to be right.


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Offlinetiptrippy
The Mechanic
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Registered: 09/09/20
Posts: 1,131
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Re: Pin in jar, or out of jar. Which is better? [Re: InnerWisdom]
    #27123969 - 01/03/21 01:36 PM (3 years, 25 days ago)

Quote:

InnerWisdom said:
I don't see how pinning would happen in the jar unless there was sufficient FAE, or the mycelium was really desperate to push out a mushroom. That happened to me in a jar of agar once.

You should know that to get a good set of pins the conditions need to be right.




100% colonization of the substrate is probably the biggest pinning trigger. Every one of my BRF jars always pinned invitro. You can even get multiple pins on a wrapped agar dish.


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OfflineInnerWisdom
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Registered: 08/09/19
Posts: 1,936
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Last seen: 4 days, 9 hours
Re: Pin in jar, or out of jar. Which is better? [Re: tiptrippy]
    #27123985 - 01/03/21 01:42 PM (3 years, 25 days ago)

ok, true, still i see no point in pinning in the jars first rather than giving it the best conditions for pinning and growing after consolidation. But I am no pf tek expert.


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Offline1kldude
Stuntzii Lover
I'm a teapot


Registered: 05/15/08
Posts: 501
Loc: Somewhere
Last seen: 2 years, 10 months
Re: Pin in jar, or out of jar. Which is better? *DELETED* [Re: InnerWisdom]
    #27124047 - 01/03/21 02:09 PM (3 years, 25 days ago)

Post deleted by 1kldude

Reason for deletion: Accidental double post


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<<<<----Any post I make is purely fictional and for informational purposes only---->>>>


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Offline1kldude
Stuntzii Lover
I'm a teapot


Registered: 05/15/08
Posts: 501
Loc: Somewhere
Last seen: 2 years, 10 months
Re: Pin in jar, or out of jar. Which is better? [Re: 1kldude]
    #27124050 - 01/03/21 02:11 PM (3 years, 25 days ago)

I would let them consolidate before fruiting.

A tip for quicker colonization is have 4 holes in the lid on the sides and 1 in the middle.

Most people just do 4 but I like to inoculate the inside too for quicker colonization and consolidation.


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<<-----|||1kldude|||----->>

<<<----------|||1 knowledgeable dude|||---------->>>

<<<<----Any post I make is purely fictional and for informational purposes only---->>>>


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InvisibleScienceDraven
Explorer
Registered: 01/03/21
Posts: 3
Re: Pin in jar, or out of jar. Which is better? [Re: tiptrippy]
    #27125998 - 01/04/21 11:56 AM (3 years, 24 days ago)

Good information. I incubated the jars in a closed environment, left mostly undisturbed. To test if I can get them to pin in the jar, I remove them from the incubator which drops their temp over 10 to 15 degrees and allows more fresh air. My experience on several jars, so far, has been they are starting the pinning activity in the jar at this point, but I'm no expert. I went ahead and put one more jar off to the side to test this theory. However, notably, the pinning activity is nearer to the top of the jar, where the holes are and the mycelium actually moved up through the vermiculite barrier.

The thought process, around "right conditions", is that it appears the pinning occurs better when the mycelium is kept nice and moist. Inside the closed jar, the mycelium is practically saturated (free water droplets are moving around inside). Where as, out of the jar, they require more attention, and dry out easier. If they get past the stage of requiring so much water before birthing, then maybe it will produce more abundant fruit, more rapidly?

Not sure, but I wanted to get your opinions. I'll follow up after further testing. I'm a bit impatient, even though I know I need to control the variables to make a good test.


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InvisibleScienceDraven
Explorer
Registered: 01/03/21
Posts: 3
Re: Pin in jar, or out of jar. Which is better? [Re: 1kldude]
    #27126010 - 01/04/21 11:58 AM (3 years, 24 days ago)

There are 4 edge holes with self-healing ports. And one center 7/32" hole with a filter, that was not inoculated.


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