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kagrenak
Stranger
Registered: 01/02/21
Posts: 2
Last seen: 2 months, 10 days
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What went wrong?
#27122036 - 01/02/21 12:47 PM (3 years, 1 month ago) |
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Hello all, new grower here. I started this some months back, like august. Things were going fine until growth just, stopped? Mycelial growth was all over the whole batch except for the surface, which had splotches of what looked like orange mycelium. I’m talking cheeto dust orange. Anyway it’s been sitting there receiving daily care, humidity monitoring, and sterile practice. I’m thinking it wasn’t sterile enough, because nothing has grown, not even pins. Some weak pins formed on the side that are shown in the pictures attached. Is this contamination, or a result of something else? I’ve scraped the forums looking for a similar post and looked up how long it takes for fruiting to make sure I’m not being impatient but idk.
I threw this out to start over, this time with jars. Just wanna know my mistake.


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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: What went wrong? [Re: kagrenak]
#27122051 - 01/02/21 12:53 PM (3 years, 1 month ago) |
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In the second pic you have a patch of mold and the surface is looking terrible even if there was no mold, so that's why they started pinning on the sides where there's better conditions. The tub is contaminated, I'd toss that and start again.
If you want you can give us a rundown on everything you did so maybe we can lend a hand and help you avoid making the same mistakes again.
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: What went wrong? [Re: kagrenak]
#27122052 - 01/02/21 12:54 PM (3 years, 1 month ago) |
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More information could get you better answers.
Did you follow a specific tek? Did you use syringe, agar, or another inoculation type? Is this outdoor grow? Fruiting chamber?
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kagrenak
Stranger
Registered: 01/02/21
Posts: 2
Last seen: 2 months, 10 days
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I used the monotub method, using a spore syringe to inject into a bag of grain spawn (with a self healing injection port). I'm trying the PF tek next since I believe it will lower the risk of contam for me personally; I should have done inoculation in my bathroom or something. I was mainly wondering what the orange patch was, and why there wasn't mycelium on top.
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brikogen
Stranger

Registered: 04/06/20
Posts: 52
Loc: Moon
Last seen: 2 years, 6 months
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Re: What went wrong? [Re: kagrenak]
#27123912 - 01/03/21 01:07 PM (3 years, 1 month ago) |
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Quote:
kagrenak said: I used the monotub method, using a spore syringe to inject into a bag of grain spawn (with a self healing injection port). I'm trying the PF tek next since I believe it will lower the risk of contam for me personally; I should have done inoculation in my bathroom or something. I was mainly wondering what the orange patch was, and why there wasn't mycelium on top.
That's your problem right there. MSS are not sterile, either start with PF and isolate or go from MSS to agar and isolate.
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