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Offlineanon0101
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Registered: 11/28/20
Posts: 60
Loc: Al’Taieu
Last seen: 2 years, 8 months
red/orange inside petri
    #27123563 - 01/03/21 10:18 AM (3 years, 25 days ago)

This is my first attempt with anything related to mushrooms besides eating them. I used four different spore syringes in 20 petri dishes. Today will be the fifth day my inoculated petri dishes have been incubating around 75-82°F.
I noticed some reddish/orange stuff inside my Dancing Tiger petri dishes. It is only in the DT. I am extremely inexperienced with inoculation etc. so i am curious if this is contamination and what exactly is it? I am trying to improve every attempt that i make at this. I used malt extract agar that i mixed with distilled water, boiled, then PC for 15 minutes. I am assuming my pd might not have been sterile even if it says they were.
Thank you in advance to anyone that reads this!




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InvisibleLemgrub
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Registered: 04/02/18
Posts: 589
Re: red/orange inside petri [Re: anon0101]
    #27123612 - 01/03/21 10:41 AM (3 years, 25 days ago)

Bacteria in your syringe. Try pf tek for that syringe.


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OfflineBallzagna
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Registered: 10/09/19
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Re: red/orange inside petri [Re: anon0101]
    #27123649 - 01/03/21 10:56 AM (3 years, 25 days ago)

definitely bacteria. you can do spore syringes to agar, just try to get one single drop of solution in the middle of the plate and isolate any clean growth. Temp range is a little high, try to keep it under like 78. Contams like the warm. Or you can try PF tek like they suggest above and then take a clone from that if it's successful but since you're already working with agar you might as well keep trying.


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No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
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Offlineanon0101
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Re: red/orange inside petri [Re: Lemgrub]
    #27123740 - 01/03/21 11:56 AM (3 years, 25 days ago)

thank you very much for the response! :smile: i will give this a try. i want to try everything.


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Offlineanon0101
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Re: red/orange inside petri [Re: Ballzagna]
    #27123751 - 01/03/21 12:03 PM (3 years, 25 days ago)

thank you for this info! i believe i put an extremely excessive amount into pd. i think i have enough of these nasties to try pf tek. i’ll turn down the temp as well! i’m a little worried about the amount of condensation and when to flip the dishes, i saw white and flipped most of them.


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