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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlinetoadlicker
member

Registered: 02/26/01
Posts: 92
Last seen: 22 years, 10 days
Re: I need help on a contam problem
    #271218 - 03/13/01 07:44 PM (22 years, 2 months ago)

I did spray the whole room with Lysol. I cleaned the whole room with antibacterial wipes first. I used a spore syringe to innoculate 12 jars that I had prepared four days earlier. There were no signs of contams before I innoculated. I used the PF style jars. The jars had a 1/4 inch vermiculite topping and a Al. foil topping over that but under the lid.

"Toad-licking is also known as lily-padding, frenching the prince and doing Kermit." Newscaster


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Offlinetoadlicker
member

Registered: 02/26/01
Posts: 92
Last seen: 22 years, 10 days
Re: I need help on a contam problem [Re: toadlicker]
    #271272 - 03/13/01 07:46 PM (22 years, 2 months ago)

What I am really looking for is a source to identify the bacteria either on the net or a book.

"Toad-licking is also known as lily-padding, frenching the prince and doing Kermit." Newscaster


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InvisibleMcMan
Pooh-Bah
Registered: 12/04/00
Posts: 661
Loc: USA
Post deleted by users_request [Re: toadlicker]
    #271281 - 03/13/01 08:07 PM (22 years, 2 months ago)



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Offlinetoadlicker
member

Registered: 02/26/01
Posts: 92
Last seen: 22 years, 10 days
Re: I need help on a contam problem [Re: McMan]
    #271301 - 03/13/01 08:37 PM (22 years, 2 months ago)

Hey McMan,
I did everything as normal. I have actually never lost a jar before. Now 100%, I am a little worried about that. What would constitute a poor preparation?

"Toad-licking is also known as lily-padding, frenching the prince and doing Kermit." Newscaster


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OfflineEddie Walker
enthusiast

Registered: 11/28/00
Posts: 142
Last seen: 21 years, 11 months
Re: I need help on a contam problem [Re: toadlicker]
    #271339 - 03/13/01 09:32 PM (22 years, 2 months ago)

Where did you get the syringe? Have you contacted the vendor? How did you sterilize your substrate?

The Hawks Eye-Fungi Perfecti


--------------------
Mycology Supplies[blue]Fungi Perfecti[/blue]


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InvisibleSoup
enthusiast
Registered: 12/12/98
Posts: 29
Re: I need help on a contam problem [Re: Eddie Walker]
    #271371 - 03/13/01 10:39 PM (22 years, 2 months ago)

Most likely a contaminated syringe. It likes to parasitize the spores of mushrooms and then when you innoculate, boom, it has all that substrate to itself. If they are your spores, then clean up your cropping and printing practices. If they came from a vendor, bitch. If they came from the free spore ring, then that just sucks because you can't really do anything.



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Offlinetoadlicker
member

Registered: 02/26/01
Posts: 92
Last seen: 22 years, 10 days
Re: I need help on a contam problem [Re: Eddie Walker]
    #271373 - 03/13/01 10:41 PM (22 years, 2 months ago)

I sterilized using a regular canning pot (not a pressure canner) and boiled the covered jars for an hour. I did contact the vendor via email last Thursday and explained exactly what happened. They ("the chicks") have not responded yet.

"Toad-licking is also known as lily-padding, frenching the prince and doing Kermit." Newscaster


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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