Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
OfflineGypsyBastard
Misanthrope
 User Gallery

Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
Re: Insane Contamination Rates on Grain Spawn. Looking For Help. [Re: Josex]
    #27122313 - 01/02/21 03:37 PM (3 years, 26 days ago)

I feel like, as others have said, it can only be 1 of 2 things. If jars are contaminating after PC but before being opened, then either
A. The sterilization process was not adequate
B. The filters are not adequate.

I would PC a jar with no filter, no poly, no MP. Just a jar of grain and a lid. Then wait. If this jar contam, it is your sterilization. If not, it's the filters. Take sometime, narrow it down to this one variable and you will have your answer and can make corrections as needed.
Like others have said, it doesn't matter what grain you are using, if things are growing this its not sterile. You just need to find out if it ever was.


Edited by GypsyBastard (01/02/21 03:38 PM)


Extras: Filter Print Post Top
InvisibleLenz
Misunderestimated
I'm a teapot User Gallery

Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Insane Contamination Rates on Grain Spawn. Looking For Help. [Re: GypsyBastard]
    #27132127 - 01/07/21 02:26 AM (3 years, 21 days ago)

Apologies for the late response, I just wanted to say thanks to everyone who chimed in, it's much appreciated, thank you all.

I've been thinking about it some more and I believe it's most likely a combination of filter failure and bad technique.


I say this because:

- I've had a couple jars contam prior to inoculation, but the majority occurs or is noticeable after inoculation. It also seems unlikely that so many of my filters would all happen to fail within a few weeks of each other.

- While I said in the OP that I haven't changed my process at all, I realized that I had started stirring my LI more frequently while pouring in order to distribute the myc more evenly.
I'm not exactly sure this could cause this much contamination, but all that air movement with the LI jar wide open wasn't too bright of me.

- I've been careless about how I shake my jars right after inoculation as well, with 25-30mL of LI I should be much more careful about not getting the SFD too wet. (Not sure if this is a real issue, from reading it seems that synthetic non cellulose filter disc's shouldn't be too susceptible to a little moisture as long as they dry out quickly).



For the time being, for peace of mind, I've re-made a bunch of lids with new SFD's, re-siliconed my LI blender attachment, PC'd 4 test jars that I'll leave sitting for a few weeks, and am planning to tighten up my SAB movement.

I think it was my mistake to have the level of confidence in my SAB procedure from having had a couple months of success. I think the fact that I had some jars contaminate prior to inoculation also skewed my perception of what was going on.
While I am still relatively confident about my SAB work, I started doing things without really thinking just because I'd been doing ok previously.
Inoculating 30-40 jars a week, I definitely got a bit lax in some ways and that certainly played a part in my recent issues.
Vigilance must be maintained lmao.

Anyways, I'll be inoculating a bunch more jars soon so I'll see how those do and hopefully won't be running into too many more issues.


--------------------
:chems:


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Crumbled Cakes as Opposed to Grain Spawn fluff_master 2,386 6 04/06/04 10:37 PM
by Magash
* Has anyone tried sorghum as grain spawn? DERRAYLD 9,594 14 12/21/18 06:31 AM
by JHOVA
* Useing popcorn as grain spawn. HunterS 3,993 6 07/06/15 09:34 PM
by Grim767
* grain spawn - which grain? felixhigh 5,229 5 10/12/18 11:07 PM
by Zero Nowhere
* Colonizing Grain Spawn for bulk?? Need a hand! Pig_In_Zen 4,408 6 09/09/20 10:48 AM
by foodguy
* Grain Spawn and Air.... HoboWilly65 1,081 3 01/23/04 11:46 PM
by Prisoner#1
* Re: Should I have a low contamination rate? Coma of Souls 974 6 01/08/00 05:25 PM
by Anonymous
* Post deleted by users_request McMan 2,479 16 07/12/01 12:42 AM
by BrownPastures

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,080 topic views. 27 members, 203 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 14 queries.