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OfflineRamJam
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Lion's Mane wierdness
    #27120329 - 01/01/21 12:26 PM (3 years, 27 days ago)

Looks like I really messed up these lion's mane G2G transfers.  I was wondering why they were taking so long to do anything.  Looks like I have grains trying to fruit on top, then contamination speedily taking over the bottom. But maybe that's myc?

I am thinking I did some of the shaking of my master too infrequently? And transferred too late? And since I have the same cobweb-looking contam in each, I guess I had that in my original jar too.  I don't think I should transfer these to bags for fruiting. Thoughts?



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Offlinetaku
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Re: Lion's Mane wierdness [Re: RamJam]
    #27120337 - 01/01/21 12:32 PM (3 years, 27 days ago)

Watching this. I'm curious because I've heard LM mycelium is more whispy and harder to see, so maybe that's how it looks? Would love knowing as well.


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Offlinetroy_white42
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Re: Lion's Mane wierdness [Re: taku]
    #27120440 - 01/01/21 01:37 PM (3 years, 27 days ago)

It is super whispy and hard to tell at first.  I could barely tell on my grains for a while and even at near full colonize.  The agar really gives you a better view of how they grow.  Heres one 10 days in.

https://ibb.co/cQZsy8F


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OfflineRamJam
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Re: Lion's Mane wierdness [Re: troy_white42]
    #27120502 - 01/01/21 02:15 PM (3 years, 27 days ago)

Ah cool, that's how my dishes look. I'm about to do some A2G transfers for some new masters.  But, I didn't recall the last master looking so whispy nor growing as fast.

But idk, should I shake and keep these jars then? I'm guessing the fruiting grains will be fine to transfer to bags along with colonized grain.


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Offlinethe man
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Re: Lion's Mane wierdness [Re: RamJam]
    #27120618 - 01/01/21 03:23 PM (3 years, 27 days ago)

ahh most likely little bacteria.. oh see u actual said that its contamed. lol ya contams cause fruiting reaction. :smile:

looks more like bacteria and myc just is wispy slowly colonizing bacterial spawn.  if doing a solid 15 psi 90-120 min must be your spawn or during transfer..

that being said, if spawning to wood prob can make it work if just bacterial for now until get clean culture.  maybe kep it incubating at slightly cooler temps aswell give myc better chance esp as it recuvers once spawned.  leave out the more obvious bacterial peaces and the uncolonized pieces.


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OfflineForresterM
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Re: Lion's Mane wierdness [Re: the man]
    #27121848 - 01/02/21 11:00 AM (3 years, 26 days ago)

That mycelium looks right, but it should have reached the bottom of the jar and fully colonized in a week or less, so either the grains were too wet or, more likely, there's bacteria in there.


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OfflineRamJam
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Re: Lion's Mane wierdness [Re: RamJam]
    #27122695 - 01/02/21 07:12 PM (3 years, 25 days ago)

Ah yep ok, could be wet grains.  This batch didn't dry out right for some reason and I was like fuck it let's see what happens cuz they weren't sticking bad. I had forgotten that, should have written it on the jars.  Guess this is what happens. Good eye Forrester..

and now my wife chimed in and said shake em up and see what happens. And I just got a new LM culture in the mail today I'm gonna nock up tomorrow, so shake it is and I'll let you guys know how that turns out lol


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OfflineRamJam
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Re: Lion's Mane wierdness [Re: the man]
    #27122697 - 01/02/21 07:13 PM (3 years, 25 days ago)

How the heck do you leave out bacterial pieces?  Go through grain by grain?  Is that actually a thing?


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Offlinethe man
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Re: Lion's Mane wierdness [Re: RamJam]
    #27122992 - 01/03/21 12:15 AM (3 years, 25 days ago)

not peice by peice but if a chunk at the bottom is bacterial and doesnt colonize or VERY slow can leave it out. not exact science, just saying if just bacterial it can still bare fruit for u and not toss it.  woods pretty resistant. ie start cultures with just cardboard and outdoor myc/mushrooms.


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OfflineRamJam
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Re: Lion's Mane wierdness [Re: RamJam]
    #27125007 - 01/03/21 11:50 PM (3 years, 24 days ago)

Huh, ok, ya I'm still so noob at all this I am happy to try stuff, even stuff I know will fail just to see it through and get a better feel for things like i'm guessing these jars will fail... unless it risks spreading contams in my shack, I have become quite fastidious in that regard haha


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OfflineRamJam
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Re: Lion's Mane wierdness [Re: RamJam]
    #27146415 - 01/13/21 06:25 PM (3 years, 15 days ago)

So I shook 3 of the jars and let one of them go, looking like i'll get some decent top fruit out of the one i didnt shake. Other 3 jars are going into bags today or tomorrow



Edited by RamJam (01/13/21 06:32 PM)


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InvisibleViolet
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Re: Lion's Mane wierdness [Re: RamJam]
    #27146566 - 01/13/21 08:22 PM (3 years, 14 days ago)

Surprised nobody mentioned yet that a2g and g2g can be a very poor way of expanding lion's mane. I originally read that in GGMM and it has turned out to be very true IME. It wants to pin rapidly in this way once it has had its hold on substrates for even just a brief time. The aerial networks of Lion's Mane are very thin and the culture typically doesn't demand full colonization of everything in its container before it will form primordia. If you get some lion's mane growth and then shake, as we might with most species, mycelium from the previously colonized grains will seek out other food sources but will also form primordia quickly as well. Pins will form from practically every individual grain.

This is why GGMM and every knowledgeable grower I've ever seen recommends starting all grain jars from liquid medium.  This has worked excellently for my countless lion's mane grows, but a2g and g2g did not. GGMM states that, with a frequent regimen of shaking every few days vigilantly before pins form, you can get around this, but there is no advantage and the pins themselves are a major disadvantage. After the grains are broken up and transferred to substrate, the battered fruit body tissue becomes a major contamination risk. Bacteria tear them right up.

Try liquid culture for lion's mane! Shake after inoculating, use before the visible mycelium begins to become more solidly white when pins will form.
Just be sure to test a few drops of each of your LCs on agar before potentially destroying your hard work preparing grains with them.

I can confidently assure you that this is good advice, and I don't even like liquid culture very much, but I grow a good bit of lion's mane for sale.


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OfflineQuadman
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Re: Lion's Mane wierdness [Re: Violet]
    #27146858 - 01/13/21 11:41 PM (3 years, 14 days ago)

Violet, it could be that a pasteurized  substrate could pose a challenge.

I've exclusively used sterilized substrates and G2G and can say that I have never had contamination problems with pins. As far as shaking, about 1 week after the the grain transfer shake I give it 1 shake and allow to fully finish colonizing.:shrug:

IMO shaking to often does more harm than good.


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InvisibleViolet
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Re: Lion's Mane wierdness [Re: Quadman]
    #27146870 - 01/13/21 11:54 PM (3 years, 14 days ago)

Well certainly there's no real risk of bacteria where bacteria don't exist, but I believe invitro pins are best avoided regardless even when they pose little threat.
Liquid culture is consistent and plenty fast, without a shake and without invitro pins (if used in time of course).  Sometime though I might try a lion's mane ryegrass seed master for g2g and see if it's more to my liking with the extra inoculation points from small grains that couldn't support much of a pin.


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Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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