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Invisiblemayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27071423 - 12/04/20 08:54 AM (3 years, 2 months ago)

A couple of related questions for those who don't use outer containers while PCing:  do you wrap the containers in anything such as foil or put 'em in naked as the day they were born?; do you tighten the lids while PCing or loosen them slightly; do you stack the HGs in the PC; how much condensation are you seeing after PCing and do you take any steps to address it or let 'em dry out naturally?

Thanks - always looking to refine the technique!!


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Exuberance is Beauty - Wm. Blake


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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan] * 1
    #27071498 - 12/04/20 09:51 AM (3 years, 2 months ago)

Quote:

mayan said:
A couple of related questions for those who don't use outer containers while PCing:  do you wrap the containers in anything such as foil or put 'em in naked as the day they were born?; do you tighten the lids while PCing or loosen them slightly; do you stack the HGs in the PC; how much condensation are you seeing after PCing and do you take any steps to address it or let 'em dry out naturally?


Thanks - always looking to refine the technique!!




I don't use an outer container.


I always pc with the lids cracked just a tiny bit. I have had two seal up and implode when I forgot. Usually they aren't a perfect seal and this won't matter, but 2 out of 34 had this issue for me.

Without an outer container you are definitely going to see some condensation. I stack up as many as will fit in the PC, or as many as I have to make, in neat stacks in a circle just like I were doing jars or whatever.

You really need to make sure the water level is below these, the boiling water can bounce them all around and if they go full sideways, agar will probably leak out.

I take them out and stack on a level surface, as tall as possible, as soon as the pressure drops and the cooker unlocks. I've stacked them more than 20 high without tipping.

In the stack, those towards the bottom and middle will have less or no condensation. Towards the top they will. It hardly bothers me. Even with 0 condensation, the view up through the bottom of these plates is superior than from the top to me, and condensation doesn't affect this.


If condensation is bothering you,
Rhizomorph and I have had success by rapidly shaking the fully closed(or VERY slight lid turn if it's not coming out when closed) plates with a wrist flick motion. The condensation works its way out of the threads and flies off.

I can't officially recommend this because it's not my tek and this just seems shady AF to me, but I've done it on like 20+ plates and haven't had a contam.


--------------------

Panaeolus Bisporus


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Invisiblemind.at.large
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27071553 - 12/04/20 10:28 AM (3 years, 2 months ago)

Thanks so much Alien for this awesome idea. I've been using these with great success and have yet to thank you so +5 for you!

Also, I don't use any containers when sterilizing. I just place them in the cooker as is. Yes, there is condensation, but not enough to worry me. Plus, I usually cook up a bunch at a time, so most of them will sit around for a week or two before getting used and by that time the condensation is cleared up.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mind.at.large]
    #27071786 - 12/04/20 12:39 PM (3 years, 2 months ago)

Quote:

mind.at.large said:
I just place them in the cooker as is. Yes, there is condensation, but not enough to worry me. Plus, I usually cook up a bunch at a time, so most of them will sit around for a week or two before getting used and by that time the condensation is cleared up.




As simple as that! outer containers are just for perfectionists idiots like us, lol.


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Invisiblemind.at.large
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27071897 - 12/04/20 01:22 PM (3 years, 2 months ago)

Quote:

Mr. Alien said:
Quote:

mind.at.large said:
I just place them in the cooker as is. Yes, there is condensation, but not enough to worry me. Plus, I usually cook up a bunch at a time, so most of them will sit around for a week or two before getting used and by that time the condensation is cleared up.




As simple as that! outer containers are just for perfectionists idiots like us, lol.



Hah! Cheers to the perfectionist idiots, from myself and the rest of the sloppy idiots! :cheersyoufuck:


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mind.at.large]
    #27072332 - 12/04/20 05:50 PM (3 years, 2 months ago)

It's true you don't need to "avoid" condensation, avoiding condensation after PC is just a whim, condensation will go away on its own. The only bad thing are the "ghosts" that condensation drops will leave after drying up that might obstruct the visibility a little.


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Invisiblemind.at.large
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27072601 - 12/04/20 08:44 PM (3 years, 2 months ago)

Quote:

Mr. Alien said:
The only bad thing are the "ghosts" that condensation drops will leave after drying up that might obstruct the visibility a little.



This is very true and very annoying. I would love to have one of those crazy tall weird christmas containers in the original post haha! Gotta hit up the thrift stores


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mind.at.large]
    #27072750 - 12/04/20 11:14 PM (3 years, 2 months ago)

Maybe the shake method to get rid of those annoying condensation drops is a good idea, just so they don't become ghosts when they dry? idk.


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Offlinezacmasters7
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27077549 - 12/07/20 06:32 PM (3 years, 2 months ago)

Hi,

Im very new and inexperienced with agar and holy grails.  Can anyone tell me what this is?  After PC, all the holy grails had condensation and half had a puddle of water at the bottom which evaporated after a week.  After the agar was dry you could see "ghosts" on the agar.  At first I thought maybe bacteria but it didn't change color.  Its very clear like crystallized sugars.  So I agreed they where good to use and made some transfers.
But now I don't know.  First picture is a transfer which has jumped over a section of the agar forming a semi circle.  And the second picture is of a unused dish that I found today,  it looks all lumpy however very clear, theres no discoloration and looks dry.  If you look at the dish straight on it looks fine, no contamination but if you angle a little light on the surface, you see the "ghosts" contam or whatever it is.

Is this some kind of bacteria or its just drying out?

Also some other questions... can I PC holy grails with my grains?  PC 90mins 15psi?  Also do the holy grails go into sealed quart jars which have the lid on?  With no GE holes.  Or no lid?


Thanks






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Offlinekarri0n
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: zacmasters7]
    #27079657 - 12/08/20 10:43 PM (3 years, 2 months ago)

Quote:

zacmasters7 said:
Hi,

Im very new and inexperienced with agar and holy grails.  Can anyone tell me what this is?  After PC, all the holy grails had condensation and half had a puddle of water at the bottom which evaporated after a week.  After the agar was dry you could see "ghosts" on the agar.  At first I thought maybe bacteria but it didn't change color.  Its very clear like crystallized sugars.  So I agreed they where good to use and made some transfers.
But now I don't know.  First picture is a transfer which has jumped over a section of the agar forming a semi circle.  And the second picture is of a unused dish that I found today,  it looks all lumpy however very clear, theres no discoloration and looks dry.  If you look at the dish straight on it looks fine, no contamination but if you angle a little light on the surface, you see the "ghosts" contam or whatever it is.

Is this some kind of bacteria or its just drying out?

Also some other questions... can I PC holy grails with my grains?  PC 90mins 15psi?  Also do the holy grails go into sealed quart jars which have the lid on?  With no GE holes.  Or no lid?


Thanks









The ones on the right kinda look like they got moved around while the agar was not yet solid. Not sure about the ones with the mycelium on them because I can't see the surface from the top down view. Mycelium will act weird if it has to crawl over water or an uneven surface though.

These don't look super thin so I don't think they would be drying out quickly.

If they didn't look like that at first and they do now, something is definitely wrong, and I'd try again.

If it's just that the surface of the agar is messed up due to shifting before gelling fully, well, I'd still try again, but I wouldn't throw out the ones that have mycelium, if it had continued to grow healthy.


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Offlinezacmasters7
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: karri0n]
    #27084446 - 12/11/20 05:56 PM (3 years, 2 months ago)

Oh okay thanks for the reply.  Since the post the mycelium has fully colonized that gap and I now do remember leaving 2 holygrails on the window sill to dry but irreverently melting the agar again so I moved them.  I'm starting to like these holy grails.


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OfflineThreads2theSoul
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: zacmasters7]
    #27095955 - 12/18/20 02:43 PM (3 years, 1 month ago)

Newb questions... don't laugh!

1) I've read somewhere on this site that it was important to keep each containers' lid and base matched. Is that the case with these containers or can they be mixed and matched per use?

2) How would I sterilize these containers? Loosely close them and PC for 20 minutes? I want to practice an actual pour.


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Offlinetombosley8
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Threads2theSoul]
    #27096409 - 12/18/20 06:51 PM (3 years, 1 month ago)

1. just don't mix brand new ones with already cooked/PC'd lids or containers. I had a problem with that one time. other than that your good to go.

2. Just do prepour with these.  You could do a pour agar with them but sounds like a huge waste of effort.
if you want to do pour agar use petri dishes. they come sterilized.


--------------------


Edited by tombosley8 (12/21/20 01:23 AM)


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Offlinezacmasters7
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Grenade01]
    #27108320 - 12/26/20 06:59 AM (3 years, 1 month ago)

Hey,

So someone chimed in and said they cool HG under a baking dish filled with water.  I also tried this
Quote:

Grenade01 said:
Quote:

jackrabbitcytoplas said:
I've placed a rectangular flat Pyrex dish full of hot water on top of the HGs...




I've used these containers myself for a good while and that is how I clear up condensation. I actually individually wrap them in cheap thin foil and wrap the whole stack in thicker foil to distribute the heat a bit, and I leave them in the thin foil and just put them all under the hot Pyrex not really knowing which ones need it and I unwrap them before using. 

I like these containers a lot although I find my glass +parafilm ones grow just a tiny bit faster.  Assuming it's from the extra gas exchange through the parafilm.  I usually use the glass for the masters and the plastic for transfers.

Quote:

Hobbit GDF said:

Same exact item




Is that a leather punch? I've tried to use mine to get a perfectly round wedge, but I can never get the myc out. Wondering if you have a trick for that?





Tried this method lastnight 8/8 NO condensation.  Was able to use them straight away.  Only problem was, looks like they boiled hard in the PC and left heavy ghosting and the agar surface is rough.  Next time will PC in jars with less time.

Mr Alien...  Have you tried the baking dish method?  I think a combination of your metal container and baking dish might give the best results.


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: zacmasters7]
    #27111125 - 12/27/20 06:18 PM (3 years, 1 month ago)

That flat Pyrex trick sounds like an excellent method to immetidately eliminate condensation! I'll give it a go next batch. I think I fucked up the agar recipe or the dark malt extract I have is too dense cause my myc is hardly growing.


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InvisibleMr. Alien
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
    #27118440 - 12/31/20 01:53 PM (3 years, 1 month ago)

Quote:

jomanda1990 said:
That flat Pyrex trick sounds like an excellent method to immetidately eliminate condensation! I'll give it a go next batch. I think I fucked up the agar recipe or the dark malt extract I have is too dense cause my myc is hardly growing.




Pictures or it didn't happen.


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Invisiblemayan
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #27121908 - 01/02/21 11:44 AM (3 years, 1 month ago)

I have used the hot water/pyrex method on a number of occasions and have found that the condensation often returns to some degree. (There may have been a batch or two that remained generally clear after the pyrex but not the majority). It seems to be variable and I have absolutely NO clue as to what conditions may help prevent returning condensation. Mainly, I have just learned to live it.  The Pyrex method is GREAT if the condensation is preventing me from effectively observing what is happening on the plate.  I also find that black agar helps with contrast if condensation/ghosts are causing issues with observation.


--------------------
Exuberance is Beauty - Wm. Blake


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OfflineSankhara
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: mayan]
    #27128142 - 01/05/21 12:30 PM (3 years, 1 month ago)

Would these qualify for this TEK? they are 1.8 inches though... Would that be too small?

https://www.milenvases.com/productos/pote-pol-30cc-pp-rosca-x10-unidades/

Argentina is so under industrialized that there are VERY few alternatives


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Offlinejomanda1990
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Sankhara]
    #27128777 - 01/05/21 05:09 PM (3 years, 1 month ago)

Quote:

Sankhara said:
Would these qualify for this TEK? they are 1.8 inches though... Would that be too small?

https://www.milenvases.com/productos/pote-pol-30cc-pp-rosca-x10-unidades/

Argentina is so under industrialized that there are VERY few alternatives




Very nice find! I wouldn't mind getting 10 or 20 of those to try them out. If they end up working fine, they are hella cheaper than the 'official' ones. I'm guessing they might have slightly worse visibility.

Anyhow, it'll have to wait two weeks or so because I'll be away from home for a while. I'll tell you how it goes once I get them, or you can tell us how it goes for you if you get them before. These could be Argentina's holy grails!


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OfflineSankhara
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jomanda1990]
    #27129091 - 01/05/21 07:07 PM (3 years, 1 month ago)

Wow! Hello brother!

Did you get to buy the "official" ones?

Yo estoy usando los potes copobras que son una cagada pero zafan.

Hope you have great holidays! I might end up giving them a chance next month as well.
Petris here are crazy expensive 3.2 usd for 10pcs....


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