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Offlinepootis
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Aesexual spore production on Penis Envy mycelium in-vitro * 1
    #27118210 - 12/31/20 12:06 PM (3 years, 28 days ago)

So I have what appears to be asexual spore production on some penis envy mycelium. I'd heard that some mushrooms do this, particularly certain oyster species, but didn't expect to see it on a psilocybe.







This is why I'm pretty confident it's not just some random contam.
Image Credit: "Growing Gourmet and Medicinal Mushrooms", 3rd edition, Paul Stamets



Stamets even writes on that page
Quote:

In culture, these forms appear as "contaminants", confusing many cultivators


:grin:

I find this to be incredibly interesting, would like to know what y'all think.

I did transfer sections from each plate to fresh agar, and I put one section from the larger plate in an LC jar. Will see what happens. Someone on discord suggested trying to culture just the black spore droplet, which is a good idea, maybe I'll get around to that some time.


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Offlinetinyshrimp
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27118270 - 12/31/20 12:35 PM (3 years, 28 days ago)

Excited to see what this post brings!


Edited by tinyshrimp (12/31/20 12:35 PM)


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Offlinepootis
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: tinyshrimp]
    #27123859 - 01/03/21 12:49 PM (3 years, 25 days ago)

The LC jar I dropped a small wedge of the PE agar in. Took the photos about 2.5 days after inoculation and I was very surprised by how much it'd grown in that short time. Seems almost suspiciously fast.






And here are the two different fresh plates I did transfers to. Both showing the kind of growth I expected although. In all cases the transferred wedges did have some of the black droplets.




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Offlinebrikogen
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27123866 - 01/03/21 12:52 PM (3 years, 25 days ago)

:threadmonitor:


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OfflineSankhara
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: brikogen]
    #27124472 - 01/03/21 06:22 PM (3 years, 25 days ago)

:mindblown:


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Offlinethe man
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Sankhara]
    #27124539 - 01/03/21 06:57 PM (3 years, 25 days ago)

crazy that they produce spores.. i wonder if viable spores? as basically have to make teh same structures in gills to make spores?


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Offlinebw86
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: the man]
    #27124579 - 01/03/21 07:22 PM (3 years, 25 days ago)

Sure is one for the books. Mushrooms never cease to amaze me.


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OfflineTight Lunchbox
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: bw86]
    #27124646 - 01/03/21 08:00 PM (3 years, 25 days ago)

For real, dude. Fucking bonkers.


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Offlinethe man
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Tight Lunchbox]
    #27124697 - 01/03/21 08:30 PM (3 years, 25 days ago)

bred for comercial production of PE spore :smile: waaaay more effective if takes less then a month!


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OfflineSankhara
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: the man]
    #27125158 - 01/04/21 03:18 AM (3 years, 24 days ago)

I'd love to see that mutation stabilized! Is there a posibility for a mislabeled print?

Also, I wonder if it ever happened to somebody before with psilocybes of course


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Offlinepootis
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Sankhara]
    #27126875 - 01/04/21 06:52 PM (3 years, 24 days ago)

Quote:

the man said:
crazy that they produce spores.. i wonder if viable spores? as basically have to make teh same structures in gills to make spores?




No reason to think they wouldn't be viable. This is a known phenomena in certain mushroom species.
I'm a bit shaky on the exact science and vocabulary but this is what you'd call an anamorph (aesexual). The teleomorph is the sexual reproduction stage in which fruiting bodies form to produce spores. The anamorphic form produces nearly microscopic stalk like structures that produce spores... like your common moulds. The spores are produced via meiosis on the spore producing structures of the teleomorph and mitosis in the anamorph.
Some species are known to have both anamorph and teleomorphic paths through their life cycle. Mycologists used to think these to be separate species, they know better now.


I only know this from 30 minutes of reading random articles or fungi blogs sourced via the gügle so whatever I got wrong hopefully a future poster will correct.

Quote:

the man said:
bred for comercial production of PE spore :smile: waaaay more effective if takes less then a month!




I have my doubts you could produce spores this way either reliably or in volume. In either case I'm far more interested in the fruiting bodies :smirk:

Quote:

Sankhara said:
I'd love to see that mutation stabilized! Is there a posibility for a mislabeled print?

Also, I wonder if it ever happened to somebody before with psilocybes of course




I'll have to educate myself better on mushroom genetics and mutation stabilisation.
I took two transfers from different sections of a multispore culture to make the two dishes photographed in the OP, so they're likely not the same culture genetically, fwiw

Possible the spores came in mislabeled but it's from Sporeworks so I doubt it

As to your last point, exactly why I posted this.

Since I now have a jar of what appears to be absolutely bangin' LC from this, can't wait to see how it behaves on grain. PC coming up to pressure right now, innoc planned for tomorrow evening....


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Offlinepootis
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27142650 - 01/11/21 08:24 PM (3 years, 17 days ago)



One (out of two) of the secondary transfer plates. Myc looking a bit cottony, really not sure what to make of it.




(3 days of growth)

Sadly the grain jars I injected with the new PE liquid culture are displaying some biology that looks decidedly un-mushroomy. Those blueish specs are definitely not trich or some other kind of mold starting to sporulate. What is it? Some type of bacterial colony perhaps?



(3 days of growth)

More of the same, note myc growing in bottom right.




(3 days of growth)

Here we see the second jar with some decent looking mycelium growing from the top. I sprayed about 5 mL of LC into the top and did not shake. Was pleased by how fast the myc grew out.



Same jar after another 3 days. (6 days of growth)
It might be 75% colonized. And definitely also infected with something. Will hang on to it and see how it develops. In the meantime I will also be starting over from scratch with the PE and germinating more spores.


Edited by pootis (01/11/21 08:32 PM)


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OfflineTstone
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27142721 - 01/11/21 09:13 PM (3 years, 17 days ago)

You have yeast contam in the jars


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Offlinepootis
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Tstone]
    #27144130 - 01/12/21 04:04 PM (3 years, 16 days ago)

Quote:

Tstone said:
You have yeast contam in the jars



First time I've noticed anything quite like it. Good to know what it is, thanks.

Does yeast typically compete heavily with mushroom mycelium or are they known to cohabitate?


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OfflineTstone
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27144227 - 01/12/21 05:07 PM (3 years, 16 days ago)

Myc will over take yeast visually, however, don't use for future g2g, tubs might smell like bread or flour. If it's all you got, give it a shot for spawn only, if you have back ups, toss. In general, I'd tos. That jar is done for any future work.

Overall, yeast contamination isn't as prevalent as trich. If, when you get it, it'll probably show up with in 2-4 days of Pc'ing the grains. My one stint with it, 1 jar out of hundreds +, was before I did g2g, as she was in line waiting for her transfer, and it showed. Honestly, less then 5% have had a yeast encounter.


Edited by Tstone (01/12/21 05:31 PM)


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OfflineTstone
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27144251 - 01/12/21 05:26 PM (3 years, 16 days ago)

Quote:

pootis said:


One (out of two) of the secondary transfer plates. Myc looking a bit cottony, really not sure what to make of it.




(3 days of growth)

Sadly the grain jars I injected with the new PE liquid culture are displaying some biology that looks decidedly un-mushroomy. Those blueish specs are definitely not trich or some other kind of mold starting to sporulate. What is it? Some type of bacterial colony perhaps?



(3 days of growth)

More of the same, note myc growing in bottom right.




(3 days of growth)

Here we see the second jar with some decent looking mycelium growing from the top. I sprayed about 5 mL of LC into the top and did not shake. Was pleased by how fast the myc grew out.



Same jar after another 3 days. (6 days of growth)
It might be 75% colonized. And definitely also infected with something. Will hang on to it and see how it develops. In the meantime I will also be starting over from scratch with the PE and germinating more spores.





Top 2 jar photos are yeast. Jar photos 3 & 4, have it, but not as prevalent , but it's there.  In 3-5 days, will look like it it is gone, as myc will grow over it, digest it, but once there, always there.


Edited by Tstone (01/12/21 05:34 PM)


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OfflineTstone
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Tstone]
    #27144278 - 01/12/21 05:48 PM (3 years, 16 days ago)

People that have never gotten it, can't identify it, but now, you know. And it won't be common for your future grows ( unless you continue to use that jar)

Scratched my head for hours, thinking about a culprit. Only thing I contemplated with, was perhaps a lid on a jar was a little loose after the PC, but when I run jars, it's 40-60 in a run, and only 1 jar got it. Only conclusion I could come up with.


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OfflineEagerNoob42
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: Tstone]
    #27144362 - 01/12/21 06:24 PM (3 years, 16 days ago)

Woah 🤔


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InvisiblebodhisattaMDiscordReddit
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: pootis]
    #27144410 - 01/12/21 06:40 PM (3 years, 16 days ago)

chlamydospores are produced by some basidiomycetes but I'm unaware of any that wouldn't require a microscope to find. They're usually hard enough to find that we're just realizing some species make them Gymnopilus Junonius for instance.

I think you simply have contamination


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Offlinebw86
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Re: Aesexual spore production on Penis Envy mycelium in-vitro [Re: bodhisatta]
    #27144413 - 01/12/21 06:42 PM (3 years, 16 days ago)

At this point thats pretty easy to see. Sure isn't anything kosher growing.


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