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odawg


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New supplementation trials : Coffee husk vs Wheat bran
#27111632 - 12/28/20 04:16 AM (3 years, 1 month ago) |
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Hi guys
I keep doing some experimentations of substrate and supplementation with the aim to sustainably grow gourmet mushrooms using a cheap method, with the lowest carbon footprint.
This week end I produced 2 different supplemented substrates in order to compare the effects of supplementations :
Specie : Pleurotus ostreatus
Strain : H1 (Belgium)
Batch #1 : - 80% Oak sawdust (got freely from a local carpenter) - 20% Coffee husk (organic - got freely from a local coffee roaster)
Batch #2 : - 80% Oak sawdust - 20% Wheat bran (organic - got from a local grain producer and miller)
Treatment : Cold pasteurization (lime bath tek)
Spawn rate : 7%
Bag weight : 4kg
I already had really good success in the past using wheat bran as supplement (reaching a BE of 100%) but it's the 1st time I use coffee husk.
Here is a photo of the coffee husk I used :

A 4kg filter bag :


Many studies successfully reported production of mushrooms using coffee husk but my main concern is to see if mushrooms can degrade toxic compounds such like caffeine and phenolic compounds.
I've read in a study that the concentration of caffeine in the coffee husk was reduced by 60.6% after mycelial growth and fruiting of Pleurotus, indicating that it was not completely degraded by the fungal culture.
I will keep you updated as incubation goes by
Edited by odawg (12/28/20 04:16 AM)
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taku
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg]
#27112011 - 12/28/20 10:36 AM (3 years, 1 month ago) |
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Could this mean caffeinated Pleurotus?! Lol
This is a great trial, watching eagerly.
--------------------
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Blackrainbow2
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: taku]
#27115263 - 12/30/20 12:22 AM (3 years, 1 month ago) |
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cool and interesting also.. why cold Cold pasteurization (lime bath tek) what advantages does that offer.. lime is not cheap unless your using oyster shells for your oyster mushroom.. the oysters Mushrooms I've been growing seem to love coffee grounds...they fuzz out in 24 hrs.. I'd swear they love caffeine.. lots of people don't like coffee and I get it mold love the hell out of coffee too...but I just PC it in a jar... throw some tissue in the jar skip agar skip grain and got to a fruiting block...
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odawg


Registered: 10/31/17
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Blackrainbow2]
#27115322 - 12/30/20 01:52 AM (3 years, 1 month ago) |
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Quote:
Blackrainbow2 said: cool and interesting also.. why cold Cold pasteurization (lime bath tek) what advantages does that offer.. lime is not cheap unless your using oyster shells for your oyster mushroom.. the oysters Mushrooms I've been growing seem to love coffee grounds...they fuzz out in 24 hrs.. I'd swear they love caffeine.. lots of people don't like coffee and I get it mold love the hell out of coffee too...but I just PC it in a jar... throw some tissue in the jar skip agar skip grain and got to a fruiting block...
Cold pasteurization is a super easy and inexpensive method to treat the substrate (for Oysters mushrooms cultivation purpose). Hydrated lime is very cheap in France (0.60 € / kg) and this tek doesn't require big quantity of lime (only 1% of the water volume).
You mention using coffee grounds which is different from coffee husk (parchment)
Here is an interesting study about it : https://www.researchgate.net/publication/26484135_Cultivation_of_Pleurotus_mushrooms_on_Brazilian_coffee_husk_and_effects_of_caffeine_and_tannic_acid
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Cyonic
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: taku]
#27117131 - 12/30/20 09:23 PM (3 years, 1 month ago) |
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Quote:
taku said: Could this mean caffeinated Pleurotus?! Lol
This is a great trial, watching eagerly. 
Actually pleuritis does assimilate the caffeine and produce caffeinated mushrooms.
It is especially interesting to think that they this might be possible with other stable alkaloids such as mescaline.
Rather than bothering with chemical extraction, cactus could be turned into oysters for a new kind of psychedelic mushrooms.
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Blackrainbow2
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Cyonic]
#27117367 - 12/31/20 12:40 AM (3 years, 1 month ago) |
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Ok makes sense to me as far as a small amount of lime and no use of fuel to heat,,if it works that's great, I've thought about using solar to heat water for that purpose but would only work in the summer.. interesting on the coffee study and I understand coffee husk and used grounds are different but booth are byproducts and can be recycles into growing media,
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Cyonic
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Blackrainbow2]
#27117957 - 12/31/20 10:25 AM (3 years, 1 month ago) |
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I've gone the chemical pasteurization route using CaOH.
It's pretty effective but you can not apply that technique with every mushroom variety. Pleurotis and cubies are both very forgiving as far as what conditions they will work in. Most fungi are more specific about what conditions they will grow in.
But that's what makes it so perfect. It creates a very selective substrate.
I actually took it to an insane level, making an incredibly caustic alkaline environment. I made a substrate that was so incredibly rich with nutrients that it really should have gotten contamination from every single angle of every single type. Not only that, but it was so rich that's it was not a saprophytes substrate. Cubensis wlcouldnt even thrive in the presence of so many nutrients, oyster would have done better, but I SATURATED the substrate in a bath of incredibly alkaline water made caustic by over saturating the bath water with CaOH.
It took for ever for the mycelium to colonize the substrate, mostly because of the richness of the substrate. I even used high nitrogen batguano that itself contained bacteria and find ngus, alfalfa, I don't remember exactly what. Various organic plant fertilizers.
Eventually it was colonized but the fruits were grotesque, mutated, bursting open and not many. It did contaminate eventually, but it would never have gotten past day two after adding spawn if it weren't for the CaOH bath.
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odawg


Registered: 10/31/17
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Cyonic]
#27132175 - 01/07/21 04:17 AM (3 years, 1 month ago) |
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Just a quick update Here are some pics of the bags
Day 3

Day 10

The mycelium grows well so far. No difference between both sub
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Stromrider
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg]
#27133139 - 01/07/21 02:44 PM (3 years, 1 month ago) |
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A lot of people have already done all these experiments and a 50/50 blend of soybean hull and sawdust blow it all out of the water for fast fruiting strains like oysters. I had to see for myself in the beginning so I understand the need to do it. You should have included a bag of that blend in the trial so you could see just how much better it is. It's quite impressive
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odawg


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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Stromrider]
#27134456 - 01/08/21 04:28 AM (3 years, 1 month ago) |
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Quote:
Stromrider said: A lot of people have already done all these experiments and a 50/50 blend of soybean hull and sawdust blow it all out of the water for fast fruiting strains like oysters. I had to see for myself in the beginning so I understand the need to do it. You should have included a bag of that blend in the trial so you could see just how much better it is. It's quite impressive
A lot of people use the MASTER MIX as substrate indeed, but they sterilize it ! On my side, I only do a cold pasteurization which makes the tek more tricky, as I can't put over 20-25% supplementation without contamination (I guess, I've never tried to get over). Moreover, here in France, I have never found soybean hull, thus I use what I can find easily around (wheat bran).
Regarding the use of coffee husk as supplementation in a substrate cold pasteurized, sorry, but I haven't found any trials here. If you have some, please share them with me.
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Stromrider
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg]
#27134870 - 01/08/21 10:21 AM (3 years, 1 month ago) |
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Oh I see. Soy hulls can definitely be a problem tto find in certain parts of the world. I buy them by the ton here.
I have not seen coffee husk trials now that I think of it. Definitely did some wheat bran and soy hull side by side personally. Wasn't even close. Report back with your coffee husk and let us know. If it doesn't go well report that. Very few people like to report bad results but it's helpful
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deadmandave
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Stromrider]
#27135103 - 01/08/21 11:57 AM (3 years, 1 month ago) |
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Do you remember your results, Stromrider? I am curious how much better soy hulls are than bran. Im in the PNW and soy hulls are rare. when i see them they are $20-30 per bag.
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Mephistophelian
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: deadmandave]
#27136506 - 01/08/21 10:11 PM (3 years, 1 month ago) |
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As a followup to deadmandave, I recall reading about how soy hulls are a huge boost in subs but never actually asked if it's a universal booster across all species or just specific ones?
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Stromrider
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Mephistophelian]
#27140082 - 01/10/21 03:49 PM (3 years, 1 month ago) |
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Quote:
deadmandave said: Do you remember your results, Stromrider? I am curious how much better soy hulls are than bran. Im in the PNW and soy hulls are rare. when i see them they are $20-30 per bag.
It was about double for me as opposed to sawdust with 20 percent wheat bran
Quote:
Mephistophelian said: As a followup to deadmandave, I recall reading about how soy hulls are a huge boost in subs but never actually asked if it's a universal booster across all species or just specific ones?
Just for fast fruiting species like oysters, lions mane, and pioppino
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odawg


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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Stromrider] 1
#27196657 - 02/10/21 02:06 AM (3 years, 6 days ago) |
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Just a quick update. I've had some issues with excessive moisture at the bottom of some bags, making difficult for the spawn to grow homogeneously. That's why I tried to use buckets instead of filter bags.
I drilled few small holes under the bucket as a way out for excess water, and holes all around to enable mushrooms to grow.
Dry substrate : 80% oak sawdust / 20% organic wheat bran
Treatment : cold pasteurisation
Incubation time : 3 weeks Then I left the bag outside (5-15°C) and sprayed water 3 times a day
The 1st primordia appeared after 8 days.
Here are some pics between day 2 and day 8 :


I am really happy with this tek which works great. Thus, I'm gonna try next time using coofee husk as supplementation (20%) in order to compare with wheat bran.
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odawg


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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg] 1
#27196702 - 02/10/21 02:50 AM (3 years, 6 days ago) |
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deadmandave
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg]
#27197097 - 02/10/21 10:22 AM (3 years, 5 days ago) |
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Nice. Im glad to see the cold past. worked even with bran. How much spawn did you use?
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0ptiquest
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: odawg]
#27198934 - 02/11/21 09:36 AM (3 years, 4 days ago) |
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Quote:
odawg said: Just a quick update. I've had some issues with excessive moisture at the bottom of some bags, making difficult for the spawn to grow homogeneously. That's why I tried to use buckets instead of filter bags.
I drilled few small holes under the bucket as a way out for excess water, and holes all around to enable mushrooms to grow.
Dry substrate : 80% oak sawdust / 20% organic wheat bran
Treatment : cold pasteurisation
Incubation time : 3 weeks Then I left the bag outside (5-15°C) and sprayed water 3 times a day
The 1st primordia appeared after 8 days.
Here are some pics between day 2 and day 8 :


I am really happy with this tek which works great. Thus, I'm gonna try next time using coofee husk as supplementation (20%) in order to compare with wheat bran.
.
Hi there, nice tek but can you explain me better the cold pasteurisation with sawdust?
I have done cold pasteurisation with straw and then let it drain the excess of water, what would you do in this case, can you give me the measurements for your tek?
thanks!
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Emre


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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: 0ptiquest]
#27199257 - 02/11/21 12:13 PM (3 years, 4 days ago) |
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Hello .. Can I grow shiitake mushrooms with cold pasteurization method? If the problem is high pH before packaging, can't we balance it with acid?
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Forrester
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Re: New supplementation trials : Coffee husk vs Wheat bran [Re: Emre]
#27199694 - 02/11/21 04:12 PM (3 years, 4 days ago) |
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Quote:
Emre said: Hello .. Can I grow shiitake mushrooms with cold pasteurization method? If the problem is high pH before packaging, can't we balance it with acid?
That depends on what you're growing them on, but please start your own thread with questions and we can help you there
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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