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OfflineSulfurshelfsean
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Hot-alkali extract?
    #27116731 - 12/30/20 05:03 PM (3 years, 29 days ago)

What is a hot alkali extract? I read about it in this study of g. Tsugae. Apparently it is a lot better method than just a hot water extract?


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InvisibleIcyurmt
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Re: Hot-alkali extract? [Re: Sulfurshelfsean]
    #27118008 - 12/31/20 10:49 AM (3 years, 28 days ago)

Researchers in this study used 5% sodium hydroxide (80°C, 6 h) for hot alkali extract. They make the clame that “the hot water extraction yields water-soluble polysaccharides, while the extraction with alkali solution is the best method to produce water-insoluble polysaccharides. Although various types of extraction method can be applied depending on the structure and water-solubility of polysaccharides, however, it is important to rupture the hard cell wall from the outer layer to the inner layer with weak-to-strong extraction conditions (pH and temperature).“ If this wasn’t the paper you were referring to in your original post, it’s worth the read.. Extraction and Fractionation of Polysaccharides from a Selected Mushroom Species, Ganoderma lucidum: A Critical Review

Here’s another study that describes the parameters for hot alkali extract from Polygonatum odoratum rather than Ganoderma as using a 10-1 sodium hydroxide to solid ratio, at 0.3M NaOH concentration (80°C for 4hr) they found the ratio of NaOH to solid was important consideration. Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome of Polygonatum odoratum


Edited by Icyurmt (12/31/20 12:42 PM)


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