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OfflineRoomfullofmirrors
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Registered: 12/29/20
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Is this agar plate bad?
    #27114456 - 12/29/20 05:24 PM (2 months, 2 days ago)

I made my first batch of agar last week. This plate looks a bit weird to me. I'm wondering what this transparent stuff is. Is it a bacteria colony or is it early mycelium? The spores came from a spore print I made i while ago.
I'm a beginner at this so I will be grateful for any tips I can get



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InvisibleDarkshadow
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Registered: 06/11/20
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Re: Is this agar plate bad? [Re: Roomfullofmirrors]
    #27115193 - 12/30/20 01:11 AM (2 months, 1 day ago)

Doesn't look good, what inoculating procedure did you use? What recipe was used for the agar?


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OfflineRoomfullofmirrors
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Re: Is this agar plate bad? [Re: Darkshadow]
    #27115337 - 12/30/20 04:12 AM (2 months, 1 day ago)

I used 3.5g of malt extract, 5g of agar and a about 2cl (0.67 oz) of lightly brewed coffee to 250ml water.
I scraped the spores with a flame sterilized needle. My other agar plates looks fine and untouched


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Offlinepatapon333
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Re: Is this agar plate bad? [Re: Roomfullofmirrors]
    #27115563 - 12/30/20 09:07 AM (2 months, 1 day ago)

search in forum for the Agar TEK - it contains exact recipes and the best method to do it the right way


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OfflineThe Mycologist
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Re: Is this agar plate bad? [Re: Roomfullofmirrors]
    #27115576 - 12/30/20 09:13 AM (2 months, 1 day ago)

I believe the clear stuff is a sign of bacteria

I see it on my plates too, I stay away from it


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OfflineRoomfullofmirrors
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Re: Is this agar plate bad? [Re: The Mycologist]
    #27116709 - 12/30/20 06:43 PM (2 months, 1 day ago)

Thanks guys. I will get rid of it and try another agar TEK next time


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InvisibleIdiotCircusBoy
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Re: Is this agar plate bad? [Re: Roomfullofmirrors]
    #27116791 - 12/30/20 07:51 PM (2 months, 23 hours ago)

Quote:

Roomfullofmirrors said:
I used 3.5g of malt extract, 5g of agar and a about 2cl (0.67 oz) of lightly brewed coffee to 250ml water.
I scraped the spores with a flame sterilized needle. My other agar plates looks fine and untouched




IMO simple is best
20g Agar
15-20g LME
1L water

Did you just scrape the spores over the dish or did you do a streak plate?


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OfflineRoomfullofmirrors
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Re: Is this agar plate bad? [Re: IdiotCircusBoy]
    #27120739 - 01/01/21 06:40 PM (1 month, 30 days ago)

I just scraped the spores onto the plate
Thanks for the recipe :laugh:


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InvisibleIdiotCircusBoy
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Re: Is this agar plate bad? [Re: Roomfullofmirrors]
    #27120869 - 01/01/21 08:06 PM (1 month, 29 days ago)

Best to do a streak plate.  Check out Bod's stuff below.

BOD's Comprehensive Agar TEK

and Video

Spore print to Agar


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