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 Roomfullofmirrors
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Registered: 12/29/20
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 Is this agar plate bad? 
#27114456 - 12/29/20 05:24 PM (2 months, 2 days ago) |
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I made my first batch of agar last week. This plate looks a bit weird to me. I'm wondering what this transparent stuff is. Is it a bacteria colony or is it early mycelium? The spores came from a spore print I made i while ago.
I'm a beginner at this so I will be grateful for any tips I can get
 
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 Darkshadow
Waiting In The Shadows
Registered: 06/11/20
Posts: 55
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Doesn't look good, what inoculating procedure did you use? What recipe was used for the agar?
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Roomfullofmirrors
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Re: Is this agar plate bad? [Re: Darkshadow]
#27115337 - 12/30/20 04:12 AM (2 months, 1 day ago) |
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I used 3.5g of malt extract, 5g of agar and a about 2cl (0.67 oz) of lightly brewed coffee to 250ml water.
I scraped the spores with a flame sterilized needle. My other agar plates looks fine and untouched
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patapon333
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Registered: 03/27/19
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search in forum for the Agar TEK - it contains exact recipes and the best method to do it the right way
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The Mycologist
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Registered: 05/06/16
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I believe the clear stuff is a sign of bacteria
I see it on my plates too, I stay away from it
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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
 
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Roomfullofmirrors
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Registered: 12/29/20
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Thanks guys. I will get rid of it and try another agar TEK next time
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IdiotCircusBoy
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Registered: 09/22/20
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Quote:
Roomfullofmirrors said:
I used 3.5g of malt extract, 5g of agar and a about 2cl (0.67 oz) of lightly brewed coffee to 250ml water.
I scraped the spores with a flame sterilized needle. My other agar plates looks fine and untouched
IMO simple is best
20g Agar
15-20g LME
1L water
Did you just scrape the spores over the dish or did you do a streak plate?
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Just call me Idiot
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Roomfullofmirrors
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Registered: 12/29/20
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Last seen: 1 month, 2 hours
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I just scraped the spores onto the plate
Thanks for the recipe 
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IdiotCircusBoy
Human Person
Registered: 09/22/20
Posts: 267
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Best to do a streak plate. Check out Bod's stuff below.
BOD's Comprehensive Agar TEK
and Video
Spore print to Agar
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Just call me Idiot
"People hasten to judge in order not to be judged themselves."
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