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23lemons
MycoNerd

Registered: 10/27/20
Posts: 30
Last seen: 3 years, 28 days
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Slow colonizing grains
#27114113 - 12/29/20 12:12 PM (3 years, 30 days ago) |
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What do you think would cause grains to take a long time to colonize?
Rye Seed:
Rinse two times - Let sit couple hours & empty the yellow color water adding fresh (repeate this process once or twice) afterward ill add some gypsum and then let the grains soak in hot water for 18 to 24 hours.
Place on the stove in my PC and boil for 10-15 mins or until I see a few grains burst.
Empty into a strainer and let all the water run off - Then place on a platic sheet and allow the grains to sit a few hours stirring around occasionally untill the outside of the grains are dry to the touch.
When they're ready I fill quart size jars 3/4th for for spore inoculation and 2/3rds full for grain transfers. Then seal the jars and place in the PC @ 15psi for 105 minutes. Once the PC is done I allow them to cool naturally in the PC over night.
The next day Ill set up my flow hood. Then spray the room down with lysol. Clean all surfaces, outside of jar & the lip around the jars with alcohol, wash hands etc.
My jars always take a long time to colonize sometimes months. Ill break them up at 25% and again at 75%. Not apl my jars will colonize either. Alot of them will just stall after I break them up and just really wont start growing again. I don't have any issues with visible contamination that I can ever see in the jars.
Some of the jars will make it to full colonization and others wont or they will look like there almost there but never finish.
The room I keep them in is always at 73F.
Trying to rule out possible issues. At first I thought it was cause I boiled my grains for half hour so I cut that time in half and am stil having issues. Ive tried numerous differnt syringes from various reputable vendors.
Could it just be the rye see Im using? Ive always used this type of rye seed and never had these issues.
Gonna add pictures below
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Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
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Re: Slow colonizing grains [Re: 23lemons]
#27114158 - 12/29/20 12:30 PM (3 years, 30 days ago) |
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Use MS on agar and agar to grain. MS syringe will almost always be contaminated. Probably has some pesky bacteria in it.
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Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 4 hours, 58 minutes
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If you have clean air to work in you can pour and use agar without issue.
If you want to work with grain you MUST learn to work with agar media.
There is no such thing as a "clean" spore print. Spores often harbor competitive organisms. "Sometimes" noc'ing grain with spores will work but it is in no way advisable and will more often fail, sometimes after you did "all" the work because everything "looked good".
Spores "work" with flour/verm cakes because there is so little space. The cube colony is able to "subdue" what may be present and kick some fruits out.
You can get any method to "work" but there is a wealth of info and willing people to help you find a way to get it to "work well consistently".
If you poured agar and noc'd plates with nice looking grains from those jars you could have clean plates in about a week or two depending on how many xfers you need to make to get there. If you do things right you could take what you have alive right now and have fruits from it in 4-6 weeks approx.
Look into agar and how to prepare it. There are lots of "teks" if you use the search function. You can get everything you need (dishes, parafilm, agar powder, light malt extract, scalpel, bunson, media bottle) online and delivered within a few days. Buying plates in case volume is the way to go and is about 80 usd for 500 plates. a roll of parafilm (skip any other "budget" wrap) lasts about as long as a case of plates.
I see way more people moving to "standard dishes" from no pour "TEK's" than the other way around. Pouring plates is not hard and cleaning cultures is usually easy.
Keep an eye on the LAGM2021 thread as there are a bunch of folks making their chronicles from start (spores to agar) to finish, starting in just a couple days. You and everyone else (~Nichrome too) could probably gleam some good and diverse knowhow from that thread. Very practical current info.
You can do it and you can do it well. Mush love fam and welcome home.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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23lemons
MycoNerd

Registered: 10/27/20
Posts: 30
Last seen: 3 years, 28 days
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Re: Slow colonizing grains [Re: Nichrome]
#27114232 - 12/29/20 01:10 PM (3 years, 30 days ago) |
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Thank you for the response.
Makes sense that the syringe is only as good as the procedures used to make it. Over the past 10 years or so I've never had any major issues like this going straight from spore to grains.
Im actually really curious to try my foot at using and cleaning cultures with agar. Where does one get that many plates for that price? I've looked around and can only find them for 0.50 - 1.00 $ per dish for disposable ones.
I'll have to search that thread and keep an eye on it to see where others make mistakes etc.
Thank you 😊
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Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
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Re: Slow colonizing grains [Re: 23lemons]
#27115481 - 12/30/20 05:53 AM (3 years, 29 days ago) |
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You can get agushy 2oz slime containers 50 for 10 bucks on amazon and make no pours, just follow a tek. Super easy. I use agar for everything.
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