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OfflineShakedown Street
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Registered: 09/05/20
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Mason jar lid question
    #27113874 - 12/29/20 09:58 AM (3 years, 30 days ago)

After reading FooMan's WBS tek and Replyom's story (who followed FooMan's Tek), I still have a question that I did not see answered in either thread.

I know that I need to put lids only --no rings--on the quart Mason jars
when I sterilize them in the PC -- with aluminum foil to keep the water out.

Every WBS tek in quart jars seems to have a SHiP lid on it just before
sterilizing it, or a quarter inch hold in the lid covered w/ micropore
tape.

Is that just for the convenience of innoculating the jars w/ a MSS when they have cooled?  Or does the mycelium need some degree of FAE while colonizing the grain inside the jar?      I ask because I plan to inoculate my sterilized grain jars inside a SAB by dropping agar.  So I don't need to perforate the lid unless the mycelium needs FAE to grow.


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 24 days
Re: Mason jar lid question [Re: Shakedown Street]
    #27113888 - 12/29/20 10:12 AM (3 years, 30 days ago)

Rings definitely go on before you PC.

Look over his Tek again, you can see that he has the rings on before he PC's his jars.

You need some sort of gas exchange filter on your jar. There are tons of lid designs nowadays, some people even use one piece unmodified plastic lids with no GE filter with success. Search around on this site or look at what some of the best growers are using and mirror that. 1/4" hole covered in a few layers of micropore tape is the go-to for some.

Yes, the ship is just for convenient inoculation with a MSS. If you're doing A2G (agar to grain), you don't need the ship lid. Even if you were inoculating your grain jars with a spore syringe (terrible idea) you could just open the lid and squirt a few drops into the jar inside your SAB.


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InvisibleTheDoobskerS
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Registered: 05/22/20
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Re: Mason jar lid question [Re: Mr. Mushie]
    #27114011 - 12/29/20 11:15 AM (3 years, 30 days ago)

Just wanna add one thing..

Just like Mushie said, MSS2G is not recommended. When you see SHIPs on a grain jar lid, they're probably using them for LC2G. Using a SHIP instead of opening the jar to squirt the syringe just leaves less possibility for contamination. With proper technique, opening the lid instead of using a SHIP will work just fine.


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OfflineProfessor X
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Re: Mason jar lid question [Re: TheDoobsker]
    #27114034 - 12/29/20 11:30 AM (3 years, 30 days ago)

If you are having lid problems try plastic. Easier to open and reusable. Just leave them loose when you PC.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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OfflineShakedown Street
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Re: Mason jar lid question [Re: Professor X]
    #27114200 - 12/29/20 12:51 PM (3 years, 30 days ago)

I understand the white plastic screw-on lids are NOT airtight. 

I have LC, mycelium. growing on grains, and myc. growing on agar.
I might use all three to inoculate these jars.  Mr. Mushie
mentioned GE as opposed to FAE.    Does that mean mycelium
growing in Mason jars just need a place to vent their air (GE),
so the air inside the jar doesn't exceed atmospheric pressure,
and  can escape the jar but the mycelia don't need FAE in their
jars as they would while fruiting?

I have some metal lids with holes, some with holes, some intact.
I also have enough white screw-on plastic lids for all my quart jars.
Maybe I will just use the screw-on plastic lids.    Are you
sure they can stand up to 15 PSI for 90 minutes in the PC ?


Edited by Shakedown Street (12/29/20 12:58 PM)


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InvisibleTheDoobskerS
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Re: Mason jar lid question [Re: Shakedown Street]
    #27115047 - 12/29/20 09:48 PM (3 years, 29 days ago)

Your jars need GE, your fruiting environment needs FAE.
Yes, if there was an airtight seal in grain jars, they would pressurize. But I think the concern is more that they wouldn't continue growth at a certain point. Same reason why you can't sit in s small airtight container for very long without losing oxygen.

The plastic lids will hold up fine in the PC. Many growers use them with or without modifications to them. If you want, just put a plastic lid on the grain jars just barely finger tight or slightly loosened; it'll provide both plenty of GE and enough of a barrier for contams.


Do some more searching though, there are countless discussions on grain jar lids and even a few summary threads


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OfflineProfessor X
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Registered: 04/18/19
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Re: Mason jar lid question [Re: TheDoobsker]
    #27115475 - 12/30/20 05:48 AM (3 years, 29 days ago)

I've run the white plastic mainstays 2.50 for 8 lids for 2 hours at 15 psi several times with no issues.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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OfflineShakedown Street
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Re: Mason jar lid question [Re: Professor X]
    #27115929 - 12/30/20 10:24 AM (3 years, 29 days ago)

Okay, Professor X, thanks for your reply.  I will try at least one in the PC.    TheDoobsker, I gather from what you said that mycelia need to breathe oxygen as we do, in order to grow.    I didn't know that.  Thank you for enlightening me.


Edited by Shakedown Street (12/30/20 10:25 AM)


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