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OfflineScandinavian
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Registered: 03/29/20
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Persistent, but selective(?) mold...
    #27113480 - 12/29/20 04:38 AM (3 years, 30 days ago)

So, it's been going steady for a while, but ever since someone trashed my grow room(you all know who) I've been having issues with moldπŸ€”

I have cleaned my room (maybe not good enough?) and even let a uvc light in the room for a few days before restarting.

Today I'm 95% sure I see the start of green mold in one of my monos again, weird thing is tho, that I used the same Coco and spawn for a bag too and that show no sign of contam πŸ€”

Regular tubs and bags are fruiting well with no contam Exept one small tub but only two monos have gotten through fruiting with no contams..

Here's what I suspect:

Monotubs aren't getting cleaned right, I clean them in a bleach solution in the shower(too weak?) then let them stand in a closet with uvc light (only 30w)for a day, then clean off with alcohol.
I have noticed tho, that there's some form of spores (i think) coming off while cleaning with alcohol πŸ€”


The room leading into my grow room may be contaminated and spores might go airborne, even tho there's no visible mold anywhere in that room.

There's one airwent and an old window (which may have some leaks around the frame, possible outside contamination? πŸ€” We've had below zero outside tho, si wouldn't expect spores to be flying around tho?


The bag to bag transfers are fast and relatively closed, while the monos are mixed, so it might be that minute or two that makes the difference? πŸ€”


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I used to know quite a bit about mycology. I also used to know my old username and email, but then I lost my mind πŸ˜” I found my mind again, but it was lacking in info when I got it back, so here I am, a noob againπŸ˜…πŸ˜’


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OfflinePBJ710
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Re: Persistent, but selective(?) mold... [Re: Scandinavian]
    #27113672 - 12/29/20 07:54 AM (3 years, 30 days ago)

Whats the inoculation source for the bags?  You can ditch the UVC light...it's not very good as disinfecting and is possibly dangerous to your eyes and skin.  Just use soapy water and then rinse it well.  If you are seeing discolorations when wiping it, you didn't clean the tub initially.


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OfflineScandinavian
I'd rather ask than fail.
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Registered: 03/29/20
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Last seen: 3 years, 28 days
Re: Persistent, but selective(?) mold... [Re: PBJ710]
    #27113702 - 12/29/20 08:13 AM (3 years, 30 days ago)

Quote:

PBJ710 said:
Whats the inoculation source for the bags?. 

If you are seeing discolorations when wiping it, you didn't clean the tub initially.





Spore/clone -> agar -> agar/water culture -> grain -> coir.

I also check the culture on another petri after last innoc. It's all clean to this point.

I'm thinking it might be the tubs, but if so, holy fuck do those spores stick well? πŸ™ˆ

Weird it's not more contaminated if the residual stuff after bleach wash(although weak solution)
Is living mold spores? πŸ€”


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I used to know quite a bit about mycology. I also used to know my old username and email, but then I lost my mind πŸ˜” I found my mind again, but it was lacking in info when I got it back, so here I am, a noob againπŸ˜…πŸ˜’


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OfflinePBJ710
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Re: Persistent, but selective(?) mold... [Re: Scandinavian]
    #27114127 - 12/29/20 12:17 PM (3 years, 30 days ago)

When the tubs trich out, does it consistently appear on the edges/bottom or just randomly on the surface/in the middle of the substrate? 

I've left some contaminated tubs WAY longer than I should have and the next round in that tub was fine after a normal scrubbing of the tub and mist/wipe with alcohol.

Another thought, are you sure your grain is sterile?  Maybe leave a bag of grain out for a week without innoculating it, then shake it and wait another week to verify it doesn't green out on it's own?  You could isolate the tub/substrate factor by letting a bag fruit invitro as well.


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OfflineProfessor X
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Registered: 04/18/19
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Re: Persistent, but selective(?) mold... [Re: PBJ710]
    #27114143 - 12/29/20 12:22 PM (3 years, 30 days ago)

I doubt it is your tub if you bleached it. In my experience 100% of the time mold is fue to dirty spawn, usually starts with bacteria or yeast which the cubes refuse to eat and lets the mold take hold.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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OfflineScandinavian
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Registered: 03/29/20
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Last seen: 3 years, 28 days
Re: Persistent, but selective(?) mold... [Re: Professor X]
    #27116974 - 12/30/20 07:44 PM (3 years, 28 days ago)

Quote:

Professor X said:
I doubt it is your tub if you bleached it. In my experience 100% of the time mold is fue to dirty spawn, usually starts with bacteria or yeast which the cubes refuse to eat and lets the mold take hold.




I'm thinking maybe they're "in the air" too.

I sterilize coir for 90min in PC, and use the same to spawn in a bag from a jar. They come out clean...

Possible i have a mold problem in the house? πŸ€”


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I used to know quite a bit about mycology. I also used to know my old username and email, but then I lost my mind πŸ˜” I found my mind again, but it was lacking in info when I got it back, so here I am, a noob againπŸ˜…πŸ˜’


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OfflinePBJ710
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Re: Persistent, but selective(?) mold... [Re: Scandinavian]
    #27117097 - 12/30/20 08:58 PM (3 years, 28 days ago)

How about some pictures of an affected tub?  Whats your grain prep/PC procedure?

Normally it's pretty difficult for household based contams to take off fast enough to get established from spore when the desired mycelium already has that much of a head start; and has the nutrition is already colonized.  The coco/verm is essentially nutritionless and won't support any growth on it's own, so it pretty much has to be coming from the grains in it.  Most likely that means your culture isn't actually clean or the grain itself isn't getting appropriately sterilized/prepped.


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Invisiblemicelio
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Re: Persistent, but selective(?) mold... [Re: Scandinavian]
    #27117153 - 12/30/20 09:44 PM (3 years, 28 days ago)

I think the problem could be, the mold Is coming from your agar dishes.

Mold has a way of being dormant on your cultures. you'll never see mold until they decide to go into their vegetation cycle..


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Invisiblemushhead
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Registered: 08/22/14
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Re: Persistent, but selective(?) mold... [Re: micelio]
    #27117235 - 12/30/20 10:40 PM (3 years, 28 days ago)

Hello germaphobe here, just my 2 cents on mold.
If you have a flow hood run it, or get an air purifier. Grab those spores out of the air first off.
You will perhaps find use in Microban, but you will need to be extremely precise in how you clean your grow area.
I would scrub every possible surface I could with hot soapy water.
Treat with microban and allow the air purifier to pickup any spores around you.
When entering your grow space attempt to be as clean as possible.
I shower before I begin work on anything agar. Yes every time I do not joke. If I don't I'm stuck thinking wtf is on my hands and arms and I just can't.
I have started keeping separate clothes to wear into my grow space that are washed separately from everything else.
Examine your technique next, recipe used, time you sterilized the agar vessel.
If your agar recipe is on spot and your plates are clear as glass but you're still getting hidden mold? Up your PC cycle by 10-15min.
I PC a 500ml distilled water, 10g agar, and 1g LME for 45min.
I can almost guarantee if done right you'll see right through em and you wont get baddies.
Now check your cultures. Mold is devious and can hide, but it is also aggressive which makes it easy to pick out.
Mold grows too fast. It's kind of unnatural how fucking fast it grows.
I have had mold overtake an entire tub before the mushroom could even get off the grain. That was a bad day. Then I joined shroomery.org
If you have a culture overtaken by mold a hot pour has gotten me out of some sticky situations.
But I would just start right the fuck over from spore, make 100% sure I'm transferring mushroom and not mold.
Follow my cleaning procedures, make sure I don't track anything in, and don't get me started on dust.
To end this rant I too last month fought off a nasty gray-white mold that snuck into everything. I got rid of it because I'm a diagnosed germaphobe who is just as crazy as this all sounds.
I'm not saying you have to do any of this btw, I just took the chance to be angry at mold.


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OfflineTigerStrike
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Registered: 12/22/20
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Re: Persistent, but selective(?) mold... [Re: micelio]
    #27117381 - 12/31/20 12:56 AM (3 years, 28 days ago)

Quote:

micelio said:
I think the problem could be, the mold Is coming from your agar dishes.

Mold has a way of being dormant on your cultures. you'll never see mold until they decide to go into their vegetation cycle..





Are you kidding,
you mean making a transfer every 2-4 days 3 or 4 times? and then mold can still be there?


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Invisiblemicelio
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Re: Persistent, but selective(?) mold... [Re: TigerStrike]
    #27117935 - 12/31/20 10:14 AM (3 years, 28 days ago)

Yes....

Read mushhead post above..

There's a reason he uses 1 gram lme to 500ml. of water and 10 grams agar.
It get your culture to move faster than the mold searching for a food source..

It took me along time to realize that all my growing problems originated from my agar dish...

Yes....! Mold can hide and be a task to get ahead of It by doing transfers..


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Invisiblemicelio
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Re: Persistent, but selective(?) mold... [Re: micelio]
    #27118256 - 12/31/20 12:28 PM (3 years, 28 days ago)

This Is great information that I suggest should be look at.


An Aloha Medicinals Scholarship month Log
Started By Seeker2be, Nov 18 2014

If you haven't seen this yet..

This is four pages of dynamite Info on how Dr.John Holliday runs his
operation at Aloha Medicinals..

https://mycotopia.net/topic/93548-an-aloha-medicinals-scholarship-month-log/



I have found this paragraph to be interesting..

    How is air quality measure?

We typically measure air quality by two methods:The first is to use a Laser Particle Counter with is done at least once a week in all the cleanrooms at Aloha Medicinals.This Laser Particle Counter is a machine that draws in air and runs it past a special laser beam counter device that can accurately count all the particles of different sizes and tells us how many particles are in each cubic foot of air.It is common to see around 1,000,000 particles per cubic foot in normal outside air.If 50% of these particles are living, that means that there are 500,000 living organisms in each cubic foot or air.Our inoculation room alone contains about 1344 cubic feet of air, or 1,244,000,000 ( ONE BILLION THREE HUNDRED AND FOURTY FOUR MILLION)particles if the air were unfiltered, or if one of the HEPA filters were faulty.Assuming that 50% of the particles are living, this means there would be 672,000,000 (SIX HUNDRED SEVENTY TWO MILLION) organism that could and would get into ur bas as we tried to inoculate them , if we did not use HEPA filter the air going into the room.


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Invisiblemushhead
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Registered: 08/22/14
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Re: Persistent, but selective(?) mold... [Re: TigerStrike] * 1
    #27118318 - 12/31/20 12:58 PM (3 years, 28 days ago)

Quote:

TigerStrike said:
Quote:

micelio said:
I think the problem could be, the mold Is coming from your agar dishes.

Mold has a way of being dormant on your cultures. you'll never see mold until they decide to go into their vegetation cycle..





Are you kidding,
you mean making a transfer every 2-4 days 3 or 4 times? and then mold can still be there?



Yes and they can even look just like the mushroom mycelia, then you move it to grain, and BAM trich, that gray-white mold that just takes over everything, green meanie, etc.
You need to make sure that on your first transfer from a germ/clone plate is 100% mycelium.
Proper use of an inoculation loop will assist you in this endeavor.


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