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AK1000
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Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin?
#27112501 - 12/28/20 02:51 PM (3 years, 1 month ago) |
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I realized recently my pasty plates were not filled up very much (maybe about 2/3rd of what pasty does it at), and I have about 20 plates varying from 2-3 weeks of colonization/consolidation time with no pins in sight. I started from a MS syringe and transferred out clean growth. I'm realizing maybe I fucked up and it won't have enough nutrients left to allow it to fruit. What do you guys think?
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cronicr



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27112504 - 12/28/20 02:54 PM (3 years, 1 month ago) |
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they take awhile to pin just grow some while ya wait
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: cronicr]
#27112593 - 12/28/20 03:41 PM (3 years, 1 month ago) |
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Quote:
cronicr said: they take awhile to pin just grow some while ya wait
Well, I'm waiting for a pin, so that I know I am growing a fruiting strain.
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cronicr



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27112597 - 12/28/20 03:44 PM (3 years, 1 month ago) |
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its been 3 weeks , you could have had a full tub ready to pin soon
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mushboy
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27112620 - 12/28/20 03:52 PM (3 years, 1 month ago) |
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Quote:
AK1000 said: What do you guys think?
My agar pins AFTER the plate has colonized. Usually around week 3 or 4.
Brf agar pins for me everytime. Like, everytime all the time.
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: cronicr]
#27112790 - 12/28/20 05:18 PM (3 years, 1 month ago) |
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Quote:
mushboy said:
Quote:
AK1000 said: What do you guys think?
My agar pins AFTER the plate has colonized. Usually around week 3 or 4.
Brf agar pins for me everytime. Like, everytime all the time.
Yea, they're colonized already.
Quote:
cronicr said: its been 3 weeks , you could have had a full tub ready to pin soon
I didn't want to just try to use mycelium that wasn't proven to fruit. Hmm, Maybe I should make some bottles and fruit them there just in case agar isn't producing.
Edited by AK1000 (12/28/20 05:23 PM)
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adhoc


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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: cronicr]
#27112807 - 12/28/20 05:33 PM (3 years, 1 month ago) |
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How common is it to get myc that won't fruit? I can't recall a thread or post about it from anyone in the 8ish months I've been here.
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99.99
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: adhoc]
#27112839 - 12/28/20 06:15 PM (3 years, 1 month ago) |
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I Isolated pins on agar thinking It would improve my grows,But I was wrong turns out if the pain takes four weeks to grow on agar It takes that long in a mono tub
I think your best bet is just to get a clean multi spore culture That way there’s enough diversity that it’s always going to do something
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Lenz
Misunderestimated


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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: adhoc]
#27112843 - 12/28/20 06:17 PM (3 years, 1 month ago) |
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Quote:
bodhisatta said: If you clone a pin of course you have a guarantee to have a fruiting culture.
I've never seen a non fruiting culture anyway.
Quote:
bodhisatta said:
You're not going to find out much about your isolate if it's going to perform shitty no matter what. Are you just making sure they fruit because chances are they will. It's pretty damn rare to run into a non fruiting strain.
Quote:
bodhisatta said: You're overthinking it and over concerned. Non fruiting strains are rare.
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99.99
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: adhoc]
#27112844 - 12/28/20 06:18 PM (3 years, 1 month ago) |
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Quote:
adhoc said: How common is it to get myc that won't fruit? I can't recall a thread or post about it from anyone in the 8ish months I've been here.
I just had to revert to old Agar plates because nothing id did would fruite anymore... waited almost 4 weeks
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mushboy
modboy



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: 99.99] 1
#27112943 - 12/28/20 07:14 PM (3 years, 1 month ago) |
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Quote:
adhoc said: How common is it to get myc that won't fruit?
Crap results can happen even with proper conditions. wont fruit means somethings up.
ive had a clone tub sit and never fruit and contamination was probably the culprit.
Edited by mushboy (12/28/20 07:16 PM)
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cronicr



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: mushboy]
#27112969 - 12/28/20 07:27 PM (3 years, 30 days ago) |
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You will know more about whats o your olate by giving it proper conditions
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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99.99
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: cronicr]
#27113196 - 12/28/20 10:17 PM (3 years, 30 days ago) |
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One thing you should do is let some of your agar plates fruit so they produce spores, that way your Always guaranteed clean spores
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: 99.99]
#27113827 - 12/29/20 09:34 AM (3 years, 30 days ago) |
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Quote:
99.99 said: One thing you should do is let some of your agar plates fruit so they produce spores, that way your Always guaranteed clean spores

Okay, looking at these plates, given how thin the agar is, it's clear that mine will have enough nutrients as they are much thicker than that. Far thinner than pasty's plates, but thicker than that, so they should have enough nutrients. Thanks for sharing the photo!
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bodhisatta 
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: adhoc]
#27113848 - 12/29/20 09:48 AM (3 years, 30 days ago) |
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Quote:
adhoc said: How common is it to get myc that won't fruit? I can't recall a thread or post about it from anyone in the 8ish months I've been here.
Can't say I've heard if it ever in 6+ years. Some people assume that they have non fruiting strains when they have poor conditions or contamination
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99.99
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: bodhisatta]
#27114017 - 12/29/20 11:19 AM (3 years, 30 days ago) |
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Quote:
bodhisatta said:
Quote:
adhoc said: How common is it to get myc that won't fruit? I can't recall a thread or post about it from anyone in the 8ish months I've been here.
Can't say I've heard if it ever in 6+ years. Some people assume that they have non fruiting strains when they have poor conditions or contamination
Ive been at this of and on for 20+ years... when i started fucking with clones it happend to me 😭
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Professor X
School for the Gifted



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: 99.99]
#27114032 - 12/29/20 11:27 AM (3 years, 30 days ago) |
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Yeah. Sometimes clone isolates won't fruit. Usually a monokaryotic strain which takes a ton of work. Contamination can be extremely sneaky. My strain pins on the plate around 7 to 10 days after full colonization, I work strictly with a ks strain isolate but have a few AA+ I have been testing lately.
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the man
still masked



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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: Professor X]
#27114102 - 12/29/20 12:08 PM (3 years, 30 days ago) |
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ya kinda jumping ahead a couple steps and doing things backwards.
no one checks a ms on agar to see if its a good fruiting var.. multispore there are a 100+ if not 1000 phenos in the dish. 1 will fruit forsure or would die off in nature. remember u have to clone the agar pin to get the same pheno. otherwise its just random. the best way is to fruit out the multispore choose a fruit or 2 to clone and go from there.
the only issue that possibly could come up is if u sectored some agar and made transfers and isolated just myc its possible that it might not fruit, but often because choosing wisely it likely will at least fruit some or colonize very quickly at very least.
see what u guys did with this agar work! folks think they need to isolate just to grow! i kid
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: the man]
#27114188 - 12/29/20 12:45 PM (3 years, 30 days ago) |
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Quote:
the man said: ya kinda jumping ahead a couple steps and doing things backwards.
no one checks a ms on agar to see if its a good fruiting var.. multispore there are a 100+ if not 1000 phenos in the dish. 1 will fruit forsure or would die off in nature. remember u have to clone the agar pin to get the same pheno. otherwise its just random. the best way is to fruit out the multispore choose a fruit or 2 to clone and go from there.
the only issue that possibly could come up is if u sectored some agar and made transfers and isolated just myc its possible that it might not fruit, but often because choosing wisely it likely will at least fruit some or colonize very quickly at very least.
see what u guys did with this agar work! folks think they need to isolate just to grow! i kid
So what I did was, 4 weeks ago, I put a drop or 2 of MS on 10 agar plates. Waited it to germinate and start colonizing. Then I scalpel'd a piece of that mycelium onto 10 new plates. And those have been colonizing for 3 weeks now. All of the agar plates are now fully colonized and I'm waiting for there to be a pin so I can transfer that to a new plate, and wait for that to colonize the agar and then make a LI with it to splish splash.
The reason I am doing it this way is because I do not want to go through the process of making grain spawn with mycelium that hasn't proven to fruit, because I had that issue in the past and I'm playing it safe to help guarantee that I'll get fruits, or so I think I am. Am I wrong with this process?
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smalltalk_canceled
Babnik


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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27114204 - 12/29/20 12:55 PM (3 years, 30 days ago) |
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Is there nothing to be learned from agar pinning?

Other than: "can fruit" and "possible contam / problem"
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (12/29/20 01:00 PM)
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: smalltalk_canceled]
#27114228 - 12/29/20 01:09 PM (3 years, 30 days ago) |
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Quote:
smalltalk_canceled said: Is there nothing to be learned from agar pinning?

Other than: "can fruit" and "possible contam / problem"
What do you mean by this question?
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smalltalk_canceled
Babnik


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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27114253 - 12/29/20 01:22 PM (3 years, 30 days ago) |
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I'm wondering aloud what info the grower may glean from agar fruiting
-------------------- Willpower is the one true virtue
  
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: smalltalk_canceled]
#27114270 - 12/29/20 01:36 PM (3 years, 30 days ago) |
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Quote:
smalltalk_canceled said: I'm wondering aloud what info the grower may glean from agar fruiting
Well, personally I'm looking to confirm that it is indeed going to produce a pin, so that I could put that pin on agar and grow that and then use it to make a tub. After that, I will have hundreds of fruits hopefully to pick the best out of to clone.
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the man
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27114345 - 12/29/20 02:23 PM (3 years, 30 days ago) |
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there was something else wrong besides genetics.. dont worry about that it wont fruit. faster just to knock up PF cakes and clone a fruit... chances are u had bacterial spawn or contam of some sort in your first attempt esp if did a MS. anyhoo do as u wish but as others have said kinda waste of time.
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AK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: the man]
#27146074 - 01/13/21 02:45 PM (3 years, 15 days ago) |
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Just to give a quick update on this and wrap up this thread: I got 3 pins this week. Took exactly 5 weeks to the day from the original plates that received a drop from the spore syringe. I just cloned them on some new plates. Life is good and patience truly pays. Thanks y'all.
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Violet


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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
#27146546 - 01/13/21 08:00 PM (3 years, 14 days ago) |
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If there's anything of possible significance to be learned from pins on agar, it's comparative consumption and pinning speed - but in the scientific sense you're really only seeing that genetic pin faster *in those conditions* which is not especially helpful when it's happening on agar (except on the rare occasion that you're doing a special agar-based grow tek like certain "poms" teks)
I'm a big fan of cloning extremely young cultures to narrow down traits before I test them in a grow-out, but since agar is not an excellent media for this, I use shallow dishes of grass seed I call "grain petris." You learn more from invitro pins this way than from agar, and the method has many other uses too!
I've heard about the possibility of a non-fruiting strain, but I've never seen it. If you use multi-spore, you'll have fruiting strains in there and wouldn't be able to tell. If you clone something, the odds are extremely high that all mycelium that grow from that tissue will be fruiting strains, and I only say "extremely high" because I personally couldn't confirm that an unmated monokaryote could contribute to a mushroom growth in the first place.
I figure to find a non-fruiting strain you'd have to isolate countless separate cultures from agar growth started with a diluted spore source and test them all out in ideal and uncontaminated conditions. A terrible waste of effort IMO that wouldn't really tell us anything. We might as well stop speculating about this possibility altogether.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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