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OfflineAK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: smalltalk_canceled]
    #27114228 - 12/29/20 01:09 PM (3 years, 30 days ago)

Quote:

smalltalk_canceled said:
Is there nothing to be learned from agar pinning?



Other than: "can fruit" and "possible contam / problem"




What do you mean by this question?


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Offlinesmalltalk_canceled
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
    #27114253 - 12/29/20 01:22 PM (3 years, 30 days ago)

I'm wondering aloud what info the grower may glean from agar fruiting


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OfflineAK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: smalltalk_canceled]
    #27114270 - 12/29/20 01:36 PM (3 years, 30 days ago)

Quote:

smalltalk_canceled said:
I'm wondering aloud what info the grower may glean from agar fruiting




Well, personally I'm looking to confirm that it is indeed going to produce a pin, so that I could put that pin on agar and grow that and then use it to make a tub. After that, I will have hundreds of fruits hopefully to pick the best out of to clone.


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Offlinethe man
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
    #27114345 - 12/29/20 02:23 PM (3 years, 30 days ago)

there was something else wrong besides genetics.. dont worry about that it wont fruit. faster just to knock up PF cakes and clone a fruit...  chances are u had bacterial spawn or contam of some sort in your first attempt esp if did a MS. anyhoo do as u wish but as others have said kinda waste of time.


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OfflineAK1000
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: the man]
    #27146074 - 01/13/21 02:45 PM (3 years, 15 days ago)

Just to give a quick update on this and wrap up this thread: I got 3 pins this week. Took exactly 5 weeks to the day from the original plates that received a drop from the spore syringe. I just cloned them on some new plates. Life is good and patience truly pays. Thanks y'all.


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InvisibleViolet
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Re: Waiting for a pin to form on agar... and the mycelium has almost eaten all the agar... will it pin? [Re: AK1000]
    #27146546 - 01/13/21 08:00 PM (3 years, 15 days ago)

If there's anything of possible significance to be learned from pins on agar, it's comparative consumption and pinning speed - but in the scientific sense you're really only seeing that genetic pin faster *in those conditions* which is not especially helpful when it's happening on agar (except on the rare occasion that you're doing a special agar-based grow tek like certain "poms" teks)

I'm a big fan of cloning extremely young cultures to narrow down traits before I test them in a grow-out, but since agar is not an excellent media for this, I use shallow dishes of grass seed I call "grain petris." You learn more from invitro pins this way than from agar, and the method has many other uses too!


I've heard about the possibility of a non-fruiting strain, but I've never seen it. If you use multi-spore, you'll have fruiting strains in there and wouldn't be able to tell. If you clone something, the odds are extremely high that all mycelium that grow from that tissue will be fruiting strains, and I only say "extremely high" because I personally couldn't confirm that an unmated monokaryote could contribute to a mushroom growth in the first place.

I figure to find a non-fruiting strain you'd have to isolate countless separate cultures from agar growth started with a diluted spore source and test them all out in ideal and uncontaminated conditions. A terrible waste of effort IMO that wouldn't really tell us anything. We might as well stop speculating about this possibility altogether.


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