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404
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Registered: 08/20/10
Posts: 14,539
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Wispy mycelium or contamination?
#27111813 - 12/28/20 08:14 AM (3 years, 1 month ago) |
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Greetings. Been quite a while since I’ve been in here.
Alright... so i got a flowhood built a few months ago, and took clones of some MSI grows of KSSS and PE. Every single transfer i have done so far has been completely clean. Below, you can see some of my latest plates. I also took some of the KSSS and made a 60ml liquid culture syringe and injected several bags which you can also see below, and taped over the holes. The liquid culture only seemed to have viable mycelium and absolutely no bacteria contamination as it would have been cloudy if it was.
The pictures of the bags are some that i’m just not sure of. I’ve only just started with liquid culture, and when i did it with oysters (also below) the mycelium started off wispy, and seems to be the case with the KSSS mycelium. Or... is it? I would like to know what you guys think. Does cube mycelium suspended in LC start off wispy sometimes?
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
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Re: Wispy mycelium or contamination? [Re: 404]
#27111821 - 12/28/20 08:19 AM (3 years, 1 month ago) |
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What is the actual question?
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404
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Registered: 08/20/10
Posts: 14,539
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Re: Wispy mycelium or contamination? [Re: bw86]
#27111847 - 12/28/20 08:39 AM (3 years, 1 month ago) |
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I can compile a list for you to make it easier.
Bottom two pics - ksss LC from clone tissue to agar to LC
>wispy cube mycelium, or contamination? >can LC mycelium look like this, especially if they are from a clone? My oyster mycelium started off wispy and was from an lc syringe, wondering if it’s a potential characteristic of the method i am using.
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404
error


Registered: 08/20/10
Posts: 14,539
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Re: Wispy mycelium or contamination? [Re: 404]
#27123338 - 01/03/21 07:35 AM (3 years, 25 days ago) |
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Going to answer my own question. All bags in question have failed, and even the ones that did pretty well with grain transfers are showing signs of issues. I guess i have to make a new thread
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jcm4620
Stranger


Registered: 05/26/19
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Re: Wispy mycelium or contamination? [Re: 404]
#27123355 - 01/03/21 07:51 AM (3 years, 25 days ago) |
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why are u putting so much grain in the bags?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Wispy mycelium or contamination? [Re: jcm4620] 2
#27123419 - 01/03/21 08:44 AM (3 years, 25 days ago) |
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Hey 404 long time no see. If you'd like send me a and I can send you some spores.
Good to see you around again and I hope you're doing well besides a couple contamed spawn bags.
Looks to be about average amount of grain to me?
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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jcm4620
Stranger


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Re: Wispy mycelium or contamination? [Re: filthyknees]
#27123627 - 01/03/21 10:50 AM (3 years, 25 days ago) |
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oh well i guess im just used to a qt worth at a time. those bags looked like they had 3-5 lbs in it and the more grain u have like that the more risk u run for improper sterilization/contamination and longer colonization time periods is all i meant
Edited by jcm4620 (01/03/21 10:50 AM)
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404
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Registered: 08/20/10
Posts: 14,539
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Re: Wispy mycelium or contamination? *DELETED* [Re: filthyknees]
#27125383 - 01/04/21 06:37 AM (3 years, 24 days ago) |
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Post deleted by 404
Reason for deletion: Dead thread
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404
error


Registered: 08/20/10
Posts: 14,539
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Re: Wispy mycelium or contamination? [Re: 404]
#27125574 - 01/04/21 08:35 AM (3 years, 24 days ago) |
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This is my flowhood by the way
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