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OfflineDocTeF
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Registered: 06/29/20
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Slightly orange tint around the cut of my transfers, HELP?
    #27110449 - 12/27/20 10:06 AM (3 years, 1 month ago)

So I recently got into agar and have been doing a lot of it, bought 500 sterilin petri dishes off ebay for £55. Probably poured about 200 petri dishes so far. At first I had a lot of contaminations before even transferring so I kept trying and now I have a pretty high success rate of my petri dishes not contaminating out.

I had a couple petri dishes of B+ and Mckennaii, they both had these little orange tints in the centre of the agar where I transferred it. The orange hasnt spead or moved but it looks like its sitting on the surface. I took pictures of everything with my phone but the pictures dont really do it justice. I made a few more transfers from both dishes and I have had amazing growth. but low and behold the more the days went on the orange started coming back, again in the centre. Im starting to think its either metabolites or my agar is drying out but I would like some opinions on it if possible.

My agar recipe is:
10g LME
10g AGAR AGAR
2 Tablespoons Activated Carbon
1g Nutritional Yeast

Im also wondering if its because my agar is maybe way too nutritious?

Any opinions or answers? shall I keep transferring to try remove it or throw them all out? or should I transfer to grains and see if they contam?

Merry Christmas and thank you all in advanced.

Pre warning some of the pictures look yellow because of the light the close up pictures are better.

DTeF



Edited by DocTeF (12/27/20 12:17 PM)


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Offlinebw86
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Registered: 11/12/06
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Re: Slightly orange tint in the centre of my transfers, HELP? [Re: DocTeF]
    #27110556 - 12/27/20 11:10 AM (3 years, 1 month ago)

So your original black agar wedge changes color after a few days?


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OfflineDocTeF
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Re: Slightly orange tint in the centre of my transfers, HELP? [Re: bw86]
    #27110567 - 12/27/20 11:16 AM (3 years, 1 month ago)

sorry I did not specify. original agar was just LMEA I transferred onto the carbon agar


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OfflineDocTeF
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: DocTeF]
    #27111054 - 12/27/20 05:29 PM (3 years, 1 month ago)

can anyone help?


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Offlinebw86
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: DocTeF]
    #27111072 - 12/27/20 05:44 PM (3 years, 1 month ago)

I don't see anything.
You can keep transferring or transfer to grains and see if they contam.


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OfflineCocaineBuffet
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: bw86]
    #27111239 - 12/27/20 07:30 PM (3 years, 1 month ago)

I can't tell if this orange tint is from the pictures/lighting/plate. They look like they all have good rhizo growth. Just stick to a single agar recipe so you have less factors at play.


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OfflineDocTeF
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: bw86]
    #27113452 - 12/29/20 03:57 AM (3 years, 30 days ago)

thanks guys, I will keep transferring out and will inoculate a few grain jars that I've had laying around for a couple days! Will keep you updated. What are your guys opinions on black agar is it worth the effort of crushing the activated carbon?


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Offlinebw86
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: DocTeF]
    #27113610 - 12/29/20 07:01 AM (3 years, 30 days ago)

no.


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OfflineDocTeF
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Re: Slightly orange tint around the cut of my transfers, HELP? [Re: bw86]
    #27114221 - 12/29/20 01:06 PM (3 years, 30 days ago)

Cheers buddy! Will revert back to the standard lmea. appreciate your help dude.


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