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1more
Ghost



Registered: 07/26/15
Posts: 25
Last seen: 2 years, 10 months
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Why am I suddenly getting tubs that looked ok going into fruiting turning green?
#27109937 - 12/27/20 01:13 AM (3 years, 1 month ago) |
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Hi all, well after two decades of trolling and learning stuff here I am finally stuck enough to ask some burning questions.... like the title says I just went from about a 90% success ratio to practically every monotub failing and looking like this:
  
Quick overview: I am doing monotubs and my grow room is the same room I am renting in a house. I am not setup for agar work yet and never have been so my process looks like ms syringe from vendor ->liquid culture (using a light malt premix from one of the vendors here) -> inoculate grain spawn (WBS if bags and RGS in jars) -> substrate in monotubs will be a coir/verm/hpoo pasteurized mix mostly but I switched to a coir/verm recipe when I got the contamination problems and I changed up the pasteurization teks troubleshooting this. Whatever i use still contams (Wagner steam tub build, PC steaming through tube, pot on stovetop tek, bucket tek....so I'm thinking that part isn't fucked or I just got the shittiest luck with the odds off all that failing)
My monotubs are stacked to the ceiling usually in the room where I sleep and never had a problem doing this. I was blaming the summer heat when I could barely keep the room at 80% but it's winter now in the desert climate so all I gotta do is crack the window if the rest of the house is too warm.
 The biggest thing I changed was from using a hepa filtered schmoovey thingy and working inside a plastic bag inside an unattached laundry room with no central air to now working in a 4x13 shed outside with a 18x24 flowhood I just built so..... maybe I need help with my clean shed room design... the flowhood has a filter from stammets site, is built to spec, and passes the flame test for laminar air so I don't suspect that. here is the lab setup I would maybe suspect my sterile technique first since it is my first flowhood ever and the workflow is different.... I'm still bleaching and chemically fucking my lungs when I knock shit up which the shed clean room area and flowhood was supposed to eliminate....
damn i'm writing a god damn book ain't I..... ok well
1.) what is that green on my subs? trich? is my room so full of mold spores now from the last 8-12 tubs going bad I gotta burn the carpet and everything in it?
1.b) How do you deal with contaminated tubs with fruits? It's too tempting to open the lid and harvest the dozen or so fruits that have grown even with the trich creeping in on it from the other side of the mono (which i have done even knowing better) instead of taking it outside and trashing the whole batch. Fruit still grows but I went from a 1 lb harvest to 1/4 oz harvests last 3 months.....
2.) How can I troubleshoot this with so many moving parts to the puzzle and no funds to buy all the agar equipment I would need to add that step to my processes? I need to get back to being able to do this: and this: and this
-------------------- Fine. One more one more time but then that's it!
 
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
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Re: Why am I suddenly getting tubs that looked ok going into fruiting turning green? [Re: 1more]
#27109943 - 12/27/20 01:21 AM (3 years, 1 month ago) |
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cuz ur spawn is dirty. id review your sterile technique and 200% use agar
Edited by jcm4620 (12/27/20 01:23 AM)
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
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Re: Why am I suddenly getting tubs that looked ok going into fruiting turning green? [Re: jcm4620]
#27109948 - 12/27/20 01:29 AM (3 years, 1 month ago) |
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u can have all the flowhoods u want and even a perfect actual lab and as long as ur still shooting dirty ass spores into a lc or anything for that matter ur gnna have problems. all spores are dirty to some extent. that is unless they were fruited invitro in a completely sterile environment. whatever is in the air where they were fruited will be on the spores. agar is the way man it dont take nothing to do especially if u got all that shit. hell i pour plates and do all my work in a still air box in a house that is full of mold so im fully confident when i say its that and that alone that is causing ur problem
the reason why u never had issues before is u simply got lucky. even a broken clock is right twice a day. personally i dont like to rely on luck. agar is the most valuable tool in this hobby. and i know u know that as the first thing u said was how ur not set up for agar. but really there is no such thing as being set up for agar. and especially if u got a flow hood ur prolly more set up than most ppl here.
Edited by jcm4620 (12/27/20 01:37 AM)
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Lemgrub



Registered: 04/02/18
Posts: 601
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Re: Why am I suddenly getting tubs that looked ok going into fruiting turning green? [Re: jcm4620]
#27109971 - 12/27/20 01:59 AM (3 years, 1 month ago) |
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Agar is easy af and cheap af. Just buy a digital scale that goes to a tenth of a gram, weigh your ingredients to ensure the proven percentages of agar density and nutrition, and buy some autoclavable (pp5,glass, etc) small containers. Ziploc makes some for $5/8 where I live. Seems daunting but you're taking extra steps elsewhere that can be cut out.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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1more
Ghost



Registered: 07/26/15
Posts: 25
Last seen: 2 years, 10 months
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Re: Why am I suddenly getting tubs that looked ok going into fruiting turning green? [Re: Lemgrub]
#27109987 - 12/27/20 02:34 AM (3 years, 1 month ago) |
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Thanks for all the responses. I know agar has been on my todo list. I bought a mini fridge just for that but then found out I wanted the model WITHOUT a freezer and so it got turned into something less mycology.
That's the third time I heard dirty spawn..... totally sucks that it does not show up in the bag or in the jar....since my latest spawn run looks like this :
I suspected my spore syringes from a vendor and went through all my lc's to produce these 10 "so far so good" BRF jars and when none of them turned green like I expected..... .... then that means maybe the contams are entering not at the LC inoculation but at the grain inoculation? That might make sense.... I did G2G for this last run but all the contaminated tubs I was basically just opening the bag of PC'd grains and pointing the needle down into it from the top..... so I'm getting new spore syringes soon and starting from there but probably I just need to pay more attention to flowhood 101 (which i've never taken) and probably agar may save me from all this time and expense and frustration. dang. You know it's different to read something and know u should b doing something different and all the reasons.... but until u fuck the pooch yourself.....
-------------------- Fine. One more one more time but then that's it!
 
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
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Re: Why am I suddenly getting tubs that looked ok going into fruiting turning green? [Re: 1more]
#27109993 - 12/27/20 03:00 AM (3 years, 1 month ago) |
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well always use a spore print when possible. syringes suck and almost always contain a shitload of bacteria. plus they just are not neat. they make a mess. sterile swabs and a print are the way to go. the swabs come in sterile packs of 2 and a box of 200 is only 10$ or so. then just swab a print then swab ur plate in a zig zag pattern like so
then transfer the areas you like to new plates
then once you find the one(s) u want and know they r clean take it to a new plate and allow to grow out to the desired amount. i personally only go about the size of a qtr or 50cent pc at the biggest
then il put that straight to my grain or in ur case LC. its as simple as that and there is no reason you shouldnt be able to do the same thing. and i promise if u do that ur grows will improve extremely.
heres a shot i use to show why u wanna use agar and how nasty spore syringes are
also brf dont make a good source for spawning to bulk. it can work ya but more often than not it will give lackluster results. also those jars look bad to me anyways
oh and keep in mind this is all pan myc so obviously ir gnna have and want different growth with cubes
Edited by jcm4620 (12/27/20 03:05 AM)
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