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OfflineMushroom Hero
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Registered: 12/26/20
Posts: 1
Last seen: 2 years, 11 months
No pour ajar slurry tek idea (looking for feedback)
    #27108901 - 12/26/20 12:43 PM (3 years, 1 month ago)

This tek is just an idea right now. I'm looking for opinions and suggestions on improving the theoretically awesome method.

1. Put a hole in 16oz mason jar lid
2. Put micropore on hole
3. Put small amount agar mixture in jar and stirrer magnet
4. Sterilize in PC
5. Flame needle on syringe and noc it up
6. Wait and watch for contams
7. Inject jar with water, measured to make it comparable to LC mixes
8. Heat agar and stir with magnet

This is just the first part. The idea is to start from agar to check for contam and then turn it into a slurry. Could pull material from it using a syringe and test on another 16oz agar jar, and if it looks good then slurry that jar too. Turn 1cc from spore syringe into unlimited inoculant.

We could then, in theory, inject grain jars with entire syringes of slurry because it is unlimited in supply. This could significantly increase colonization rate. The high speed could make it more difficult to spot contams, but the no-lid-opening type method here could hopefully help to prevent contams.

Ive heard that agar to lc works very quickly, so that combined with full syringe grain injections could potentially be a very fast method that also uses large quantities all the while using no-pour to make it very easy.

I think this tek has a lot of potential for easy cheap quick unlimited innoculant right out the gate from first theoretical syringe, but I am a newbie and would like some input on how to improve the theory.

I chose a hypothetical 16oz jar because it would allow one to simultaneously have more agar surface, ie wider jar, without making the agar puddle deeper, ie keep it as flat and 2d as possible. Also allows for much more slurry per jar, while only using a little bit more agar mixture per jar.

Any ideas on improving the slurry ingredients? Ie adding honey or other ingredients with the water and measuring to make it as hospitable to growth as possible.

Any other input is appreciated. Thanks!


Edited by Mushroom Hero (12/26/20 12:52 PM)


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OfflineWizardSJ
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Registered: 08/20/20
Posts: 29
Last seen: 5 months, 18 days
Re: No pour ajar slurry tek idea (looking for feedback) [Re: Mushroom Hero]
    #27109210 - 12/26/20 03:24 PM (3 years, 1 month ago)

Heating the agar to melting temp may kill the agar. Every step up until heating is a no pour agar method i use with great success. I would reccomend going agar to lc, dont see the need for melting and mixing. You could make hundreds of LC jars from one agar dish.


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